Our research group is interested in the design of innovative agricultural goods using genomics, biotechnology and synthetic biology tools
Our main experimental system is the tomato fruit, and our aim is to increase its added value with new shapes, colors, aromas and healthy properties. For this, we browse the natural variability of tomato-related wild species in order to identify loci conferring fruit quality traits. Once those loci are identified, we use marker-assisted breeding to transfer them to modern tomato cultivars. Moving beyond the limits imposed by sexual hybridization and natural variability, we also make use of biotechnology tools as multigene engineering and genetic transformation to bring new quality traits into the tomato genome.
In a second line of research, we employ Synthetic Biology tools and principles for engineering plant biofactories of added-value products. In our lab, we have learnt how to build increasingly complex genetic instructions at the DNA level. By introducing these instructions into the genome of solanaceae species as tomato, tobacco and especially Nicotiana benthamiana (a tobacco relative), our goal is to re-direct the genetic program of the plant towards the synthesis of added-value products. Assisted by Agrobacterium tumefaciens and sometimes by viral vectors, we learn how to produce high levels of fine chemicals as antibodies, lectins, or snake anti-venoms in a short period of time.
For more information, visit our group webpage at http://pgb.ibmcp.csic.es
RESEARCH INTERESTS
Vazquez-Vilar V, Fernandez-del-Carmen A, Garcia-Carpintero V, Drapal M, Presa S, Ricci D, Diretto G, Rambla JL, Fernandez-Muñoz R, Ana Espinosa-Ruiz A, Fraser PD, Martin C, Granell A, Orzaez D. (2023) Dually biofortified cisgenic tomatoes with increased flavonoids and branched-chain amino acids content. bioRxiv 2022.10.31.514528
Moreno-Giménez E, Gandía M, Sáez Z, Manzanares P, Yenush L, Orzáez D, Marcos JF, Garrigues S. (2023) FungalBraid 2.0: expanding the synthetic biology toolbox for the biotechnological exploitation of filamentous fungi. Front Bioeng Biotechnol. 11:1222812.
Ranawaka B, An J, Lorenc MT, Jung H, Sulli M, Aprea G, Roden S, Llaca V, Hayashi S, Asadyar L, LeBlanc Z, Ahmed Z, Naim F, de Campos SB, Cooper T, de Felippes FF, Dong P, Zhong S, Garcia-Carpintero V, Orzaez D, Dudley KJ, Bombarely A, Bally J, Winefield C, Giuliano G, Waterhouse PM. (2023) A multi-omic Nicotiana benthamiana resource for fundamental research and biotechnology. Nat Plants. 2023 Aug 10.
De Paola C, Garcia-Carpintero V, Vazquez-Vilar M, Kaminski K, Fernandez-Del-Carmen A, Sierro N, Ivanov NV, Giuliano G, Waterhouse P, Orzaez D. (2023) Comparative analysis of the Squamosa Promoter Binding-Like (SPL) gene family in Nicotiana benthamiana and Nicotiana tabacum. Plant Sci. 335:111797.
Vazquez-Vilar M, Selma S, Orzaez D. (2023) The design of synthetic gene circuits in plants: new components, old challenges. J Exp Bot. 74(13):3791-3805.
Kallam K, Moreno-Giménez E, Mateos-Fernández R, Tansley C, Gianoglio S, Orzaez D, Patron N. (2023) Tunable control of insect pheromone biosynthesis in Nicotiana benthamiana. Plant Biotechnol J. 21(7):1440-1453.
Juteršek M, Petek M, Ramšak Ž, Moreno-Giménez E, Gianoglio S, Mateos-Fernández R, Orzáez D, Gruden K, Baebler Š. (2022) Transcriptional deregulation of stress-growth balance in Nicotiana benthamiana biofactories producing insect sex pheromones. Front Plant Sci. 13:941338.
Moreno-Giménez E, Selma S, Calvache C, Orzáez D. (2022) GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for Fine-Tuned Recombinant Gene Expression in Plants. ACS Synth Biol. 11(9):3037-3048. Erratum in: ACS Synth Biol.11(12):4226.
Gianoglio S, Comino C, Moglia A, Acquadro A, García-Carpintero V, Diretto G, Sevi F, Rambla JL, Dono G, Valentino D, Moreno-Giménez E, Fullana-Pericàs M, Conesa MA, Galmés J, Lanteri S, Mazzucato A, Orzáez D, Granell A. (2022) In-Depth Characterization of greenflesh Tomato Mutants Obtained by CRISPR/Cas9 Editing: A Case Study With Implications for Breeding and Regulation. Front Plant Sci. 13:936089.
Selma S, Gianoglio S, Uranga M, Vázquez-Vilar M, Espinosa-Ruiz A, Drapal M, Fraser PD, Daròs JA, Orzáez D. (2022) Potato virus X-delivered CRISPR activation programs lead to strong endogenous gene induction and transient metabolic reprogramming in Nicotiana benthamiana. Plant J. 111(6):1550-1564.
González B, Vazquez-Vilar M, Sánchez-Vicente J, Orzáez D. (2022) Optimization of Vectors and Targeting Strategies Including GoldenBraid and Genome Editing Tools: GoldenBraid Assembly of Multiplex CRISPR /Cas12a Guide RNAs for Gene Editing in Nicotiana benthamiana. Methods Mol Biol. 2480:193-214.
Selma S, Sanmartín N, Espinosa-Ruiz A, Gianoglio S, Lopez-Gresa MP, Vázquez-Vilar M, Flors V, Granell A, Orzaez D. (2022) Custom-made design of metabolite composition in N. benthamiana leaves using CRISPR activators. Plant Biotechnol J. 20(8):1578-1590.
Garcia-Perez E, Diego-Martin B, Quijano-Rubio A, Moreno-Giménez E, Selma S, Orzaez D, Vazquez-Vilar M. (2022)
A copper switch for inducing CRISPR/Cas9-based transcriptional activation tightly regulates gene expression in Nicotiana benthamiana.
BMC Biotechnol. 22(1):12.
Pizzio GA, Mayordomo C, Lozano-Juste J, Garcia-Carpintero V, Vazquez-Vilar M, Nebauer SG, Kaminski KP, Ivanov NV, Estevez JC, Rivera-Moreno M, Albert A, Orzaez D, Rodriguez PL. (2022)
PYL1- and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue.
Cells. 11(5):795.
Calvache C, Vazquez-Vilar M, Selma S, Uranga M, Fernández-Del-Carmen A, Daròs JA, Orzáez D. (2022)
Strong and tunable anti-CRISPR/Cas activities in plants.
Plant Biotechnol J. 20(2):399-408.
Vazquez-Vilar M, Juarez P, Bernabé-Orts JM, Orzaez D. (2022)
Design of Multiplexing CRISPR/Cas9 Constructs for Plant Genome Engineering Using the GoldenBraid DNA Assembly Standard.
Methods Mol Biol. 2379:27-44.
Mateos Fernández R, Petek M, Gerasymenko I, Juteršek M, Baebler Š, Kallam K, Moreno Giménez E, Gondolf J, Nordmann A, Gruden K, Orzaez D, Patron NJ. (2022)
Insect pest management in the age of synthetic biology.
Plant Biotechnol J. 20(1):25-36.
Uranga M, Vazquez-Vilar M, Orzáez D, Daròs JA. (2021)
CRISPR-Cas12a Genome Editing at the Whole-Plant Level Using Two Compatible RNA Virus Vectors.
CRISPR J. 4(5):761-769.
Molina-Hidalgo FJ, Vazquez-Vilar M, D’Andrea L, Demurtas OC, Fraser P, Giuliano G, Bock R, Orzáez D, Goossens A. (2021)
Engineering Metabolism in Nicotiana Species: A Promising Future.
Trends Biotechnol. 39(9):901-913.
Christou P, Dhingra A, Slamet-Loedin IH, Oliveira M, Chakraborty S, Buyel J, Stoger E, Schillberg S, Orzaez D, Quemada H. (2021)
Preface: Genome editing in plants.
Transgenic Res. 30(4):317-320.
Selma S, Orzáez D. (2021)
Perspectives for epigenetic editing in crops.
Transgenic Res. 30(4):381-400.
Vazquez-Vilar M, Garcia-Carpintero V, Selma S, Bernabé-Orts JM, Sanchez-Vicente J, Salazar-Sarasua B, Ressa A, de Paola C, Ajenjo M, Quintela JC, Fernández-Del-Carmen A, Granell A, Orzáez D. (2021)
The GB4.0 Platform, an All-In-One Tool for CRISPR/Cas-Based Multiplex Genome Engineering in Plants.
Front Plant Sci. 12:689937, doi.org/10.3389/fpls.2021.689937
Uranga M, Aragonés V, Selma S, Vázquez-Vilar M, Orzáez D, Daròs JA. (2021)
Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector.
Plant J. 106(2):555-565
Diego-Martin B, González B, Vazquez-Vilar M, Selma S, Mateos-Fernández R, Gianoglio S, Fernández-Del-Carmen A, Orzáez D. (2020)
Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities.
Front Plant Sci. 11:612781. doi: 10.3389/fpls.2020.612781.
Maioli A, Gianoglio S, Moglia A, Acquadro A, Valentino D, Milani AM, Prohens J, Orzaez D, Granell A, Lanteri S, Comino C. (2020)
Simultaneous CRISPR/Cas9 Editing of Three PPO Genes Reduces Fruit Flesh Browning in Solanum melongena L.
Front Plant Sci. 11:607161. doi: 10.3389/fpls.2020.607161.
Bernabé-Orts JM, Quijano-Rubio A, Vazquez-Vilar M, Mancheño-Bonillo J, Moles-Casas V, Selma S, Gianoglio S, Granell A, Orzaez D (2020)
A memory switch for plant synthetic biology based on the phage ϕC31 integration system.
Nucleic Acids Res. 48(6):3379-3394
Dono G, Rambla JL, Frusciante S, Granell A, Diretto G, Mazzucato A (2020)
Color Mutations Alter the Biochemical Composition in the San Marzano Tomato Fruit.
Metabolites 10(3):110
Rambla JL, Granell A (2020)
Determination of Plant Volatile Apocarotenoids
Methods Mol Biol. 2083:165-175
Gonzalo MJ, Li YC, Chen KY, Gil D, Montoro T, Nájera I, Baixauli C, Granell A, Monforte AJ (2020)
Genetic Control of Reproductive Traits in Tomatoes Under High Temperature
Front Plant Sci. 11:326
Conesa MÀ, Fullana-Pericàs M, Granell A, Galmés J (2020)
Mediterranean Long Shelf-Life Landraces: An Untapped Genetic Resource for Tomato Improvement
Front Plant Sci. 10:1651
Vazquez-Vilar M, Gandía M, García-Carpintero V, Marqués E2, Sarrion-Perdigones A, Yenush L, Polaina J, Manzanares P, Marcos JF, Orzaez D (2020)
Multigene Engineering by GoldenBraid Cloning: From Plants to Filamentous Fungi and Beyond
Curr Protoc Mol Biol 130(1):e116
Breia R, Conde A, Conde C, Fortes AM, Granell A, Gerós H (2020)
VvERD6l13 is a grapevine sucrose transporter highly up-regulated in response to infection by Botrytis cinerea and Erysiphe necator
Plant Physiol Biochem. 154:508-516
Breia R, Conde A, Pimentel D, Conde C, Fortes AM, Granell A, Gerós H (2020)
VvSWEET7 Is a Mono- and Disaccharide Transporter Up-Regulated in Response to Botrytis cinerea Infection in Grape Berries
Front Plant Sci. 10:1753
Bernabé-Orts JM, Casas-Rodrigo I, Minguet EG, Landolfi V, Garcia-Carpintero V, Gianoglio S, Vázquez-Vilar M, Granell A, Orzaez D (2019)
Assessment of Cas12a-mediated gene editing efficiency in plants
Plant Biotechnol J. 10:1971-1984
Aliaga-Franco N, Zhang C, Presa S, Srivastava AK, Granell A, Alabadí D, Sadanandom A, Blázquez MA, Minguet EG (2019)
Identification of Transgene-Free CRISPR-Edited Plants of Rice, Tomato, and Arabidopsis by Monitoring DsRED Fluorescence in Dry Seeds
Front Plant Sci 10:1150
Selma S, Bernabé-Orts JM, Vazquez-Vilar M, Diego-Martin B, Ajenjo M, Garcia-Carpintero V, Granell A, Orzaez D (2019)
Strong gene activation in plants with genome-wide specificity using a new orthogonal CRISPR/Cas9-based programmable transcriptional activator
Plant Biotechnol J. 9:1703-1705
González-Mas MC, Rambla JL, López-Gresa MP, Blázquez MA, Granell A. (2019)
Volatile Compounds in Citrus Essential Oils: A Comprehensive Review
Front Plant Sci 10:12
Vazquez-Vilar M, Orzaez D, Patron N. (2018)
DNA assembly standards: Setting the low-level programming code for plant biotechnology.
Plant Sci. 273:33-41
Hamza R, Pérez-Hedo M, Urbaneja A, Rambla JL, Granell A, Gaddour K, Beltrán JP, Cañas LA (2018)
Expression of two barley proteinase inhibitors in tomato promotes endogenous defensive response and enhances resistance to Tuta absoluta
BMC Plant Biol. 18(1):24
Esteras C, Rambla JL, Sánchez G, López-Gresa MP, González-Mas MC, Fernández-Trujillo JP, Bellés JM, Granell A, Picó MB (2018)
Fruit flesh volatile and carotenoid profile analysis within the Cucumis melo L. species reveals unexploited variability for future genetic breeding
J Sci Food Agric.98(10):3915-3925
Hernanz-Koers M, Gandía M, Garrigues S, Manzanares P, Yenush L, Orzaez D, Marcos JF (2018)
FungalBraid: A GoldenBraid-based modular cloning platform for the assembly and exchange of DNA elements tailored to fungal synthetic biology
Fungal Genet Biol;116:51-61
Rubio-Cabetas MJ, Pons C, Bielsa B, Amador ML, Marti C, Granell A (2018)
Preformed and induced mechanisms underlies the differential responses of Prunus rootstock to hypoxia
J Plant Physiol. 228:134-149
Tieman D, Zhu G, Resende MF Jr, Lin T, Nguyen C, Bies D, Rambla JL, Beltran KS, Taylor M, Zhang B, Ikeda H, Liu Z, Fisher J, Zemach I, Monforte A, Zamir D, Granell A, Kirst M, Huang S, Klee H (2017)
A chemical genetic roadmap to improved tomato flavor
Science 355(6323):391-394
López-Gresa MP, Lisón P, Campos L, Rodrigo I, Rambla JL, Granell A, Conejero V, Bellés JM (2017)
A Non-targeted Metabolomics Approach Unravels the VOCs Associated with the Tomato Immune Response against Pseudomonas syringae
Front Plant Sci. doi: 10.3389/fpls.2017.01188
Julve Parreño JM, Huet E, Fernández-Del-Carmen A, Segura A, Venturi M, Gandía A, Pan WS, Albaladejo I, Forment J, Pla D, Wigdorovitz A, Calvete JJ, Gutiérrez C, Gutiérrez JM, Granell A, Orzáez D (2017)
A Synthetic Biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins
Plant Biotechnol J. doi: 10.1111/pbi.12823.
Ahrazem O, Diretto G, Argandoña J, Rubio-Moraga Á, Julve JM, Orzáez D, Granell A, Gómez-Gómez L. (2017)
Evolutionarily distinct carotenoid cleavage dioxygenases are responsible for crocetin production in Buddleja davidii
J Exp Bot. 68(16):4663-4677
Vazquez-Vilar M, Quijano-Rubio A, Fernandez-Del-Carmen A, Sarrion-Perdigones A, Ochoa-Fernandez R, Ziarsolo P, Blanca J, Granell A, Orzaez D (2017)
GB3.0: a platform for plant bio-design that connects functional DNA elements with associated biological data
Nucleic Acids Res. 45(4): 2196–2209
Urrutia M, Rambla JL, Alexiou KG, Granell A, Monfort A (2017)
Genetic analysis of the wild strawberry (Fragaria vesca) volatile composition
Plant Physiol Biochem. 121:99-11
Rambla JL, Medina A, Fernández-Del-Carmen A, Barrantes W, Grandillo S, Cammareri M, López-Casado G, Rodrigo G, Alonso A, García-Martínez S, Primo J, Ruiz JJ, Fernández-Muñoz R, Monforte AJ, Granell A (2017)
Identification, introgression, and validation of fruit volatile QTLs from a red-fruited wild tomato species
J Exp Bot. 68(3):429-442
Corrales AR, Carrillo L, Lasierra P, Nebauer SG, Dominguez-Figueroa J, Renau-Morata B, Pollmann S, Granell A, Molina RV, Vicente-Carbajosa J, Medina J (2017)
Multifaceted Role of Cycling Dof Factor 3 (CDF3) in the regulation of flowering time and abiotic stress responses in Arabidopsis
Plant Cell Environ. 40:748–764
Pasin F, Bedoya LC, Bernabé-Orts JM, Gallo A, Simón-Mateo C, Orzaez D, García JA (2017)
Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins
ACS Synth Biol. 6(10):1962-1968
Fernandez-Moreno JP, Levy-Samoha D, Malitsky S, Monforte AJ, Orzaez D, Aharoni A, Granell A (2017)
Uncovering tomato quantitative trait loci and candidate genes for fruit cuticular lipid composition using the Solanum pennellii introgression line population
J Exp Bot. 68(11):2703-2716
Gascuel Q, Diretto G, Monforte AJ, Fortes AM, Granell A (2017)
Use of Natural Diversity and Biotechnology to Increase the Quality and Nutritional Content of Tomato and Grape
Front Plant Sci. 8:652
Besada C, Sanchez G, Gil R, Granell A, Salvador A (2017)
Volatile metabolite profiling reveals the changes in the volatile compounds of new spontaneously generated loquat cultivars
Food Res Int. 11:234-243
Poyatos-Pertíñez S, Quinet M, Ortíz-Atienza A, Yuste-Lisbona FJ, Pons C, Giménez E, Angosto T, Granell A, Capel J, Lozano R. (2016)
A Factor Linking Floral Organ Identity and Growth Revealed by Characterization of the Tomato Mutant unfinished flower development (ufd).
Front Plant Sci. 7:1648
Pons C, Martí C, Forment J, Crisosto CH, Dandekar AM, Granell A (2016)
A genetic genomics-expression approach reveals components of the molecular mechanisms beyond the cell wall that underlie peach fruit woolliness due to cold storage
Plant Mol Biol. 92(4-5):483-503
Escobar-Bravo R, Alba JM, Pons C, Granell A, Kant MR, Moriones E, Fernández-Muñoz R (2016)
A Jasmonate-Inducible Defense Trait Transferred from Wild into Cultivated Tomato Establishes Increased Whitefly Resistance and Reduced Viral Disease Incidence
Front Plant Sci. 7:1732
Vazquez-Vilar M, Miquel Bernabe-Orts J, Fernandez-del-Carmen A, Ziarsolo P, Blanca J, Granel, A, Orzaez D (2016)
A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard
Plant Methods 12: 10
Fernandez-Moreno JP, Malitsky S, Lashbrooke J, Biswa AK, Racovita RC, Mellerowicz EJ, Jetter R, Orzaez, D, Aharoni A, Granel A (2016)
An efficient method for medium throughput screening of cuticular wax composition in different plant species
Metabolomics 12: 73
Breitel DA, Chappell-Maor L, Meir S, Panizel I, Pons C, Hao Y, Yifhar T, Yasuor, Zouine M, Bouzayen M, Granell A; Rogachev I, Aharon, A (2016)
AUXIN RESPONSE FACTOR 2 Intersects Hormonal Signals in the Regulation of Tomato Fruit Ripening
Plos Genetics 12
Juarez P, Virdi V, Depicker A, Orzaez D. (2016)
Biomanufacturing of protective antibodies and other therapeutics in edible plant tissues for oral applications.
Plant Biotechnol J. 14(9):1791-9
Fernandez-Moreno JP, Tzfadia O, Forment J, Presa S, Rogachev I, Meir S, Orzaez D, Aharoni A, Granell A (2016)
Characterization of a New Pink-Fruited Tomato Mutant Results in the Identification of a Null Allele of the SlMYB12 Transcription Factor.
Plant physiology 171: 1821-1836
Racovita RC, Hen-Avivi S, Fernandez-Moreno JP, Granell A, Aharoni A, Jetter R. (2016)
Composition of cuticular waxes coating flag leaf blades and peduncles of Triticum aestivum cv. Bethlehem.
Phytochemistry 130:182-92
Polturak Guy, Breitel D, Grossman N, Sarrion-Perdigones A, Weithorn E, Pliner M, Orzaez D, Granell A, Rogachev I, Aharon A (2016)
Elucidation of the first committed step in betalain biosynthesis enables the heterologous engineering of betalain pigments in plants
New Phytologist 210: 269-283
Polturak G, Breitel D, Sarrion-Perdigones A, Grossman N, Weithorn E, Pliner M, Orzaez D, Granell A, Rogachev I, Aharoni A (2016)
Elucidation of the first step in betalain biosynthesis allows the heterologous production of betalain pigments in plants
Planta Med. 81(S 01):S1-S381
Barrantes W, López-Casado G, García-Martínez S, Alonso A, Rubio F, Ruiz JJ, Fernández-Muñoz R, Granell A, Monforte AJ. (2016)
Exploring New Alleles Involved in Tomato Fruit Quality in an Introgression Line Library of Solanum pimpinellifolium
Front Plant Sci. 7:1172
Rambla JL, Trapero-Mozos A, Diretto G, Rubio-Moraga A, Granell A, Gómez-Gómez L, Ahrazem O. (2016)
Gene-Metabolite Networks of Volatile Metabolism in Airen and Tempranillo Grape Cultivars Revealed a Distinct Mechanism of Aroma Bouquet Production
Front Plant Sci. 7:1619
Barrero-Gil J, Huertas R, Rambla JL, Granell A, Salinas J (2016)
Tomato plants increase their tolerance to low temperature in a chilling acclimation process entailing comprehensive transcriptional and metabolic adjustments
Plant Cell Environ. 39(10):2303-18
Dicker M, Tschofen M, Maresch D, König J, Juarez P, Orzaez D, Altmann F, Steinkellner H, Strasser R (2016)
Transient Glyco-Engineering to Produce Recombinant IgA1 with Defined N- and O-Glycans in Plants
Frontiers in Plant Science 7:18
Pons C, Dagar A, Marti C, Singh V, Crisosto CH, Friedman H, Lurie S, Granell A (2015)
Pre-symptomatic transcriptome changes during cold storage of chilling sensitive and resistant peach cultivars to elucidate chilling injury mechanisms
BMC Genomics 16: 245
Patron NJ, Orzaez D, Marillonnet S, Warzecha H, Matthewman C, Youles M, Raitskin O, Leveau A, Farré G, Rogers C, Smith A, Hibberd J, Webb AA, Locke J, Schornack S, Ajioka J, Baulcombe DC, Zipfel C, Kamoun S, Jones JD, Kuhn H, Robatzek S, Van Esse HP,Sanders D, Oldroyd G, Martin C, Field R, O’Connor S, Fox S, Wulff B, Miller B, Breakspear A, Radhakrishnan G, Delaux PM, Loqué D, Granell A, Tissier A, Shih P, Brutnell TP, Quick WP, Rischer H, Fraser PD, Aharoni A, Raines C, South PF, Ané JM, Hamberger BR, Langdale J, Stougaard J, Bouwmeester H, Udvardi M, Murray JA, Ntoukakis V, Schäfer P, Denby K, Edwards KJ, Osbourn A, Haseloff J (2015)
Standards for plant synthetic biology: a common syntax for exchange of DNA parts
New Phytologist 208: 13-19
Lashbrooke J, Adato Al,Lotan O,Alkan N, Tsimbalist T, Rechav K, Fernandez-Moreno JP, Widemann E, Grausem B, Pinot F, Granell A, Costa F, Aharoni A (2015)
The Tomato MIXTA-Like Transcription Factor Coordinates Fruit Epidermis Conical Cell Development and Cuticular Lipid Biosynthesis and Assembly
Plant Physiology 169: 2553-2571
Rambla JL, Alfaro C, Medina A, Zarzo M, Primo J, Granell A (2015)
Tomato fruit volatile profiles are highly dependent on sample processing and capturing methods
Metabolomics 11: 1708-1720
Capel C, Fernandez del Carmen A, Alba JM, Lima-Silva V, Hernández-Gras F, Salinas M, Boronat A, Angosto T, Botella MA, Fernández-Muñoz R, Granell A, Capel J, Lozano R (2015)
Wide-genome QTL mapping of fruit quality traits in a tomato RIL population derived from the wild-relative species Solanum pimpinellifolium L.
Theoretical and Applied Genetics 128: 2019-2035
Pons C, Marti C, Forment J, Crisosto CH, Dandekar Abhaya M, Granell A (2014)
A Bulk Segregant Gene Expression Analysis of a Peach Population Reveals Components of the Underlying Mechanism of the Fruit Cold Response
PLOS ONE 9
Corrales AR, Nebauer SG, Carrillo L, Fernandez-Nohales P, Marques J, Renau-Morata B, Granell A, Pollmann S, Vicente-Carbajosa J, Molina RV, Medina J (2014)
Characterization of tomato Cycling Dof Factors reveals conserved and new functions in the control of flowering time and abiotic stress responses
JOURNAL OF EXPERIMENTAL BOTANY 65: 995-1012
Sarrion-Perdigones A, Palaci J, Granell A, Orzaez D (2014)
Design and construction of multigenic constructs for plant biotechnology using the GoldenBraid cloning strategy.
Methods in molecular biology 1116: 133-151
Juarez P, Fernandez-Del-Carmen A, Rambla JL, Presa S, Mico A, Granell A, Orzaez D (2014)
Evaluation of Unintended Effects in the Composition of Tomatoes Expressing a Human Immunoglobulin A against Rotavirus
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 62: 8158-8168
Seymour GB, Granell A (2014)
Fruit development and ripening
Journal of Experimental Botany 65:4489–4490
Rambla JL, Gonzalez-Mas MC, Pons C, Bernet GP, Asins MJ, Granell A (2014)
Fruit volatile profiles of two citrus hybrids are dramatically different from those of their parents.
Journal of agricultural and food chemistry 62: 11312-11322
Cocaliadis MF, Fernandez-Munoz R, Pons C, Orzaez D, Granell A (2014)
Increasing tomato fruit quality by enhancing fruit chloroplast function. A double-edged sword?
Journal of experimental botany 65: 4589-4598
Rubio-Moraga A, Rambla JL, Fernandez-de-Carmen A,Trapero-Mozos A, Ahrazem O, Orzaez D, Granell A, Gomez-Gomez L (2014)
New target carotenoids for CCD4 enzymes are revealed with the characterization of a novel stress-induced carotenoid cleavage dioxygenase gene from Crocus sativus
PLANT MOLECULAR BIOLOGY 86: 555-569
Campos L, Granell P, Tárraga S, López-Gresa MP, Conejero V, Bellés JM, Rodrigo I, Lisón P (2014)
Salicylic acid and gentisic acid induce RNA silencing-related genes and plant resistance to RNA pathogens
Plant Physiology and Biochemistry 77: 35-43
Rambla JL, Tikunov YM, Monforte AJ, Bovy AG, Granell A (2014)
The expanded tomato fruit volatile landscape.
Journal of experimental botany 65: 4613-4623
Manuel Julve J, Gandia A, Fernandez-del-Carmen A, Sarrion-Perdigones A, Castelijns B, Granell A, Orzaez D (2013)
A coat-independent superinfection exclusion rapidly imposed in Nicotiana benthamiana cells by tobacco mosaic virus is not prevented by depletion of the movement protein
Plant Molecular Biology 81:553-564
Besada C, Salvador A, Sdiri S, Gil R, Granell A (2013)
A combination of physiological and chemometrics analyses reveals the main associations between quality and ripening traits and volatiles in two loquat cultivars
Metabolomics 9: 324-336
Herranz MC, Niehl A, Rosales M, Fiore N, Zamorano A, Granell A, Pallas V (2013)
A remarkable synergistic effect at the transcriptomic level in peach fruits doubly infected by Prunus necrotic ringspot virus and Peach latent mosaic viroid
Virology Journal 10: 1-15
Hueso Estornell L, Pons C, Martinez A, Enrique O’Connor J, Orzaez D, Granell A (2013)
A VIN1 GUS::GFP fusion reveals activated sucrose metabolism programming occurring in interspersed cells during tomato fruit ripening
Journal Of Plant Physiology 170: 1113-1121
Sanchez G, Venegas-Caleron M, Salas JJ, Monforte A, Badenes ML, Granell A (2013)
An integrative “omics” approach identifies new candidate genes to impact aroma volatiles in peach fruit
Bmc Genomics 14
Zhang Y, Butelli E, De Stefano R, Schoonbeek HJ, Magusin A, Pagliarani C, Wellner N, Hill L, Orzaez D, Granell A, Jones JDG, Martin C (2013)
Anthocyanins Double the Shelf Life of Tomatoes by Delaying Overripening and Reducing Susceptibility to Gray Mold
Current Biology 23: 1094-1100
Juarez P, Huet-Trujillo E, Sarrion-Perdigones A, Elvira Falconi E, Granell A, Orzaez D (2013)
Combinatorial Analysis of Secretory Immunoglobulin A (sIgA) Expression in Plants
International Journal Of Molecular Sciences 14: 6205-6222
Dagar A, Pons Puig C, Marti Ibanez C, Ziliotto F, Bonghi C, Crisosto CH, Friedman H, Lurie S, Granell A (2013)
Comparative transcript profiling of a peach and its nectarine mutant at harvest reveals differences in gene expression related to storability
Tree Genetics & Genomes 9: 223-235
Rubio Moraga A, Ahrazem O, Rambla JL, Granell A, Gomez Gomez L (2013)
Crocins with High Levels of Sugar Conjugation Contribute to the Yellow Colours of Early-Spring Flowering Crocus Tepals
Plos One 8
Aragueez I, Hoffmann T, Osorio S, Rambla JL, Medina-Escobar N, Granell A, Botella MA, Schwab W, Valpuesta V (2013)
Eugenol Production in Achenes and Receptacles of Strawberry Fruits Is Catalyzed by Synthases Exhibiting Distinct Kinetics
Plant Physiology 163: 946-958
Sarrion-Perdigones A, Vazquez-
Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D (2013)
GoldenBraid 2.0: A Comprehensive DNA Assembly Framework for Plant Synthetic Biology
Plant Physiology 162: 1618-1631
Tikunov YM, Molthoff J, de Vos RC H Beekwilder J, van Houwelingen A, van der Hooft JJ, J Nijenhuis-de Vries M, Labrie CW, Verkerke W, van de Geest H, Zamora MV, Presa S, Rambla JL, Granell A, Hall RD, Bovy AG (2013)
NON-SMOKY GLYCOSYLTRANSFERASE1 Prevents the Release of Smoky Aroma from Tomato Fruit
Plant Cell 25: 3067-3078
Fernandez-del-Carmen A, Juarez P, Presa S, Granell A, Orzaez D (2013)
Recombinant jacalin-like plant lectins are produced at high levels in Nicotiana benthamiana and retain agglutination activity and sugar specificity
Journal Of Biotechnology 163:391-400
Besada C, Lopez MP, Salvador A, Granell A (2013)
Tannins are implied in flesh browning of persimmon
Acta Horticulturae 996: 411-416
Shi Jian X, Adato Al, Alkan N, He Y, Lashbrooke J, Matas AJ, Meir S, Malitsky S, Isaacson T, Prusky D, Leshkowitz D, Schreiber L, Granell AR, Widemann E, Grausem B, Pinot F, Rose Jocelyn KC, Rogachev I, Rothan C, Aharoni A (2013)
The tomato SlSHINE3 transcription factor regulates fruit cuticle formation and epidermal patterning
New Phytologist 197: 468-480
Fernandez-Moreno JP, Orzaez D, Granell A (2013)
VIGS: a tool to study fruit development in Solanum lycopersicum.
Methods In Molecular Biology (Clifton N.J.) 975:183-196
Besada C, Sanchez G, Salvador A, Granell A (2013)
Volatile compounds associated to the loss of astringency in persimmon fruit revealed by untargeted GC-MS analysis
Metabolomics 9: 157-172
Mounet F, Moing A, Kowalczyk M, Rohrmann J, Petit J, Garcia V, Maucourt M, Yano K, Deborde C, Aoki K, Berges H, Granell A, Fernie AR, Bellini C, Rothan C, Lemaire-Chamley M (2012)
Down-regulation of a single auxin efflux transport protein in tomato induces precocious fruit development
Journal of Experimental Botany 63: 4901-4917
Zorrilla-Fontanesi Y, Rambla JL, Cabeza A, Medina JJ, Sanchez-Sevilla JF, Valpuesta V, Botella MA, Granell A, Amaya I (2012)
Genetic Analysis of Strawberry Fruit Aroma and Identification of O-Methyltransferase FaOMT as the Locus Controlling Natural Variation in Mesifurane Content
Plant Physiology 159: 851-870
Lima-Silva V, Rosado A, Amorim-Silva V, Munoz-Merida A, Pons C, Bombarely A, Trelles O, Fernandez-Munoz R, Granell A, Valpuesta V, Botella MA (2012)
Genetic and genome-wide transcriptomic analyses identify co-regulation of oxidative response and hormone transcript abundance with vitamin C content in tomato fruit
BMC GENOMICS 13: 187
Juarez P, Presa S, Espi J, Pineda B, Anton MT, Moreno V, Buesa J, Granell A, Orzaez D (2012)
Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes
Plant Biotechnology Journal 10: 341-352
Powell ALT, Nguyen CV, Hill T, Cheng KL, Figueroa-Balderas R, Aktas H, Ashrafi H, Pons C, Fernandez-Munoz R, Vicente A, Lopez-Baltazar J, Barry CS, Liu YS, Chetelat R, Granell A, Van Deynze A, Giovannoni JJ, Bennett AB (2012)
Uniform ripening Encodes a Golden 2-like Transcription Factor Regulating Tomato Fruit Chloroplast Development
Science 336: 1711-1715
Rambla JL, Vera-Sirera F, Blázquez MA, Carbonell J, Granell A (2010)
Quantitation of biogenic tetraamines in Arabidopsis thaliana
Analytical Biochemistry 397: 208-211
Estornell LH, Orzaez D, Lopez-Pena L, Pineda B, Anton MT, Moreno V, Granell A (2009)
A multisite gateway-based toolkit for targeted gene expression and hairpin RNA silencing in tomato fruits
Plant Biotechnology Journal 7: 298-309
Mueller LA, Lankhorst RK,…, Botella MA, Perez-Alonso M, Fernandez-Pedrosa V, Osorio S, Mico A, Granell A,…, Zamir D, Stiekema W (2009)
A snapshot of the emerging tomato genome sequence
The Plant Genome 2: 1-15
Orzaez D, Medina A, Torre S, Fernandez-Moreno JP, Rambla JL, Fernandez-Del-Carmen A, Butelli E, Martin C, Granell A (2009)
A visual reporter system for VIGS in tomato fruit based on anthocyanin accumulation
Plant Physiology 150: 1122-1134
Ballester A-R, Molthoff J, de Vos R, Hekkert BtL, Orzaez D, Fernandez-Moreno J-P, Tripodi P, Grandillo S, Martin C, Heldens , Ykema M, Granell A, Bovy A (2009)
Biochemical and molecular analysis of pink tomatoes: deregulated expression of the gene encoding transcription factor SlMYB12 leads to pink tomato fruit colour
Plant Physiology 152: 71-84
Orzáez D, Granell A, Blázquez MA (2009)
Manufacturing antibodies in the plant cell
Biotechnology Journal 4: 1712-1724
Zanor MI, Rambla-Nebot JL, Chaib J, Agnes S, Granell A, Fernie AR, Causse M (2009)
Metabolic characterisation of loci affecting sensory attributes in tomato allows an assessment of the influence of the levels of primary metabolites and volatile organic contents
Journal of Experimental Botany 60: 2139-2145
Rubio-Moraga A, Rambla JL, Ahrazem O, Granell A, Gómez-Gómez L (2009)
Metabolite and target transcript analyses during stigma development in Crocus sativus
Phytochemistry 70: 1009-1016
Birney E, Hudson TJ, Green ED, Gunter C, Eddy S, Rogers J, Harris JR, Ehrlich SD, Apweiler R, Austin CP, Berglund L, Bobrow M, Bountra C, Brookes AJ, Cambon-Thomsen A, Carter NP, Chisholm RL, Contreras JL, Cooke RM, Crosby WL, Dewar K, Durbin R, Dyke SO, Ecker JR, El Emam K, Feuk L, Gabriel SB, Gallacher J, Gelbart WM, Granell A, Guarner F, Hubbard T, Jackson SA, Jennings JL, Joly Y, Jones SM, Kaye J, Kennedy KL, Knoppers BM, Kyrpides NC, Lowrance WW, Luo J, MacKay JJ, Martín-Rivera L, McCombie WR, McPherson JD, Miller L, Miller W, Moerman D, Mooser V, Morton CC, Ostell JM, Ouellette BF, Parkhill J, Raina PS, Rawlings C, Scherer SE, Scherer SW, Schofield PN, Sensen CW, Stodden VC, Sussman MR, Tanaka T, Thornton J, Tsunoda T, Valle D, Vuorio EI, Walker NM, Wallace S, Weinstock G, Whitman WB, Worley KC, Wu C, Wu J, Yu J (2009)
Prepublication data sharing. Toronto International Data Release Workshop
Nature 461: 168-70
Orzaez D, Granell A (2009)
Reverse genetics and transient gene expression in fleshy fruits: Overcoming plant stable transformation
Plant Signaling and Behaviour 4: 864-7
Rubio A, Rambla JL, Santaella M, Gómez MD, Orzáez D, Granell A, Gómez-Gómez L. (2008)
Cytosolicand plastoglobule-targeted carotenoid dioxigenases from Crocus sativus are both involved in beta-ionone release
J Biol Chem 283 (36): 24816-25
Ogundiwin EA, Marti C, Forment J, Pons C, Granell A, Gradziel TM, Peace CP, Crisosto CH (2008)
Development of ChillPeach genomic tools and identification of cold-responsive genes in peach fruit
Plant Molecular Biology 68: 379-97
Marti C, Orzáez D, Ellul P, Moreno V, Carbonell J, Granell A. (2007)
Silencing of DELLA induces facultative parthenocarpy in tomato fruits
Plant Journal 52 (5): 865-876
Sanchez-Ballesta MT, Zacarias L, Granell A, Lafuente MT (2007)
β-1,3-glucanase gene expression as a molecular marker for postharvest physiological disorders in Citrus fruit and its hormonal regulation
Postharvest Biology and Biotechnology 48: 146-149
OrzaezD, Mirabel S, Wilemein W, Granell A (2006)
Agroinjection of tomato fruits: a tool for rapid functional analysis of transgenes directly in fruit
Plant Physiology 140: 3-11
Sanchez Ballesta MT, Granell A, Zacarias L, Lafuente T (2006)
Characterization of a beta-1,3-glucanase from citrus fruit as related to chilling-induced injury and ethylene production
Postharvest Biology and Biotechnology 40: 133-140
Ko K, Taylor D, Paulo Argenton P, Innes J, Pedram B, Seiberg S, Granell A, Ko Z (2005)
Evidence that the Plastid Translocon Tic40 Components Possess Modulating Capabilities
Journal of Biological Chemistry 280: 215-224
Mueller, Tanksley SD, Giovannoni JJ,…, Granell A, Botella MA, Giuliano G, Fruciante L , Causse M, Zamir D (2005)
The Tomato Sequencing Project, the first cornerstone of the International Solanaceae Project (SOL)
Comparative and Functional Genomics 5: 153-158
Labate MTV, Ko K, Ko ZW, Costa Pinto LSR, Real MJUD, Ribeiro Romano M, Roxo Barja P, Granell A, Friso G, Van Wijk KJ, Brugnoli E, Labate CA (2004)
Constitutive Expression of Pea Lhcb1–2 in Tobacco Affects Plant Development, Morphology and Photosynthetic Capacity
Plant Molecular Biology 55: 701-714
Sanchez-Ballesta MT, Rodrigo MJ, Lafuente MT, Granell A, Zacarias L (2004)
Dehydrin from Citrus, which confers in vitro dehydration and freezing protection activity is constitutive and highly expressed in the flavedo of fruit but responsive to cold and water stress in leaves
Journal of Agricultural and Food Chemistry 52: 1950-57
Sanchez-Ballesta MT, Zacarias L, Granell A, Lafuente MT (2000)
Accumulation of PAL transcript and PAL activity as affected by heat-conditioning and low-temperature storage and its relation to chilling sensitivity in mandarin fruits
Journal of Agricultural and Food Chemistry 48: 2726-2731
Sanchez-Ballesta MT, Lafuente MT, Zacarias L, Granell A (2000)
Involvement of phenylalanine ammonia-lyase in the response of Fortune mandarin fruits to cold temperature
Physiologia Plantarum 108: 382-389
Orzaez D, Blay R, Granell A (1999)
Programme of senescence in petals and carpels of pisum sativum l. Flowers and its control by ethylene
Planta 208: 220-226
Orzaez D, Granell A (1997)
DNA fragmentation is regulated by ethylene during carpel senescence in Pisum sativum
Plant Journal 11: 137-144
Orzaez D, Granell A (1997)
The plant homologue of the defender against apoptotic death gene is down-regulated during senescence of flower petals
FEBS Letters 404: 275-278
Alonso JM, Chamarro J, Granell A (1995)
A non-photosynthetic ferredoxin gene is induced by ethylene in Citrus organs
Plant Molecular Biology 29: 1211-1221
Alonso JM, Granell A (1995)
A putative vacuolar processing protease is regulated by ethylene and also during fruit ripening in citrus-fruit
Plant Physiology 109: 541-547
Alonso JM, Chamarro J, Granell A (1995)
Evidence for the involvement of ethylene in the expression of specific rnas during maturation of the orange, a nonclimacteric fruit
Plant Molecular Biology 29: 385-390
TANHACO2: Production of thermostable carbonic anhydrases in tobacco biofactory crops for CO2 capture (TED2021-131171B-I00). Project Start: December 2022. Project end: November 2024. Duration: 48 months
PHEROPLUS: Sustainable bio-production of insect pheromones and their precursors (PLEC2021-008020). Project Start: December 2021. Project end: November 2024. Duration: 36 months
Entidad financiadora: MCIN/AEI/10.13039/501100011033 and European Union “NextGenerationEU”/PRTR.
IP: Dr. Diego Orzáez (Project Coordinator)
Phytoapps2.0: Exploiting the Modular Architecture of CRISPR/Cas to design programmablegene circuits and other new breeding tools in plants (PID2019-108203RB-I00) (2020-2022)
SUSPHIRE: Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture.Project Start: June 2018. Project end: May 2021. Duration: 36 months
Project Website: http://susphire.info
Entidad financiadora: ERA CoBioTech partners: BMBF, MIZS, AEI, CDTI, UKRI-BBSRC, co-funded by the European Union.
IP: Dr. Diego Orzáez (Project Coordinator)
NEWCOTIANA: Developing Multipurpose Nicotiana Crops for Molecular Farming using New Plant Breeding Techniques (GA No. 760331) (2018-2022)
IP: IP CSIC: Dr Diego Orzaez; Project Coordinator:Julian Ma
Phytoapps: Design of synthetic orthogonal regulatory gene circuits in plants using CRISPR-Cas9-like programmable DNA-binding proteins. (2017 – 2019)
IP: Dr. Diego Orzaez, Prof Antonio Granell
TomGEM: A holistic multi-actor approach towards the design of new tomato varieties and management practices to improve yield and quality in the face of climate change. (2016-2019)
IP: Prof Antonio Granell; Project Coordinator: Prof. Mondher Bouzayen
TRADITOM (634561). Traditional tomato varieties and cultural practicies: a case for agricultural diversification with impact on food security and health of European population. (2015 – 2018)
IP: Prof Antonio Granell (Project Coordinator)
Green switches: Design of synthetic gene circuits for recombinant protein production and nutritional enhancement in Solanaceous plants. (2014 – 2016)
IP: Dr. Diego Orzaez, Prof Antonio Granell
Production of recombinant polyclonal antibodies in Nicotiana benthamiana plants in an experimental greenhouse. (2014)
IP: Dr. Diego Orzaez
Production of Therapeutic Proteins in Plant Biofactories – PROTEBIOV. (2011 – 2014)
IP CSIC: Dr Diego Orzaez. Project Coordinator: IDEN BIOTECHNOLOGY S.L.
Engineering Tomatoes for Health: Building Blocks for Intragenesis and Molecular Farming in Solanaceae. (2011- 2013)
IP: Dr. Diego Orzaez
Nuevos productos cosméticos y sanitarios mediante producción recombinante. (2015 – 2017)
TANHACO2: Production of thermostable carbonic anhydrases in tobacco biofactory crops for CO2 capture (TED2021-131171B-I00). Project Start: December 2022. Project end: November 2024. Duration: 48 months
PHEROPLUS: Sustainable bio-production of insect pheromones and their precursors (PLEC2021-008020). Project Start: December 2021. Project end: November 2024. Duration: 36 months
Entidad financiadora: MCIN/AEI/10.13039/501100011033 and European Union “NextGenerationEU”/PRTR.
IP: Dr. Diego Orzáez (Project Coordinator)
Phytoapps2.0: Exploiting the Modular Architecture of CRISPR/Cas to design programmablegene circuits and other new breeding tools in plants (PID2019-108203RB-I00) (2020-2022)
SUSPHIRE: Sustainable Bioproduction of Pheromones for Insect Pest Control in Agriculture.Project Start: June 2018. Project end: May 2021. Duration: 36 months
Project Website: http://susphire.info
Entidad financiadora: ERA CoBioTech partners: BMBF, MIZS, AEI, CDTI, UKRI-BBSRC, co-funded by the European Union.
IP: Dr. Diego Orzáez (Project Coordinator)
NEWCOTIANA: Developing Multipurpose Nicotiana Crops for Molecular Farming using New Plant Breeding Techniques (GA No. 760331) (2018-2022)
IP: IP CSIC: Dr Diego Orzaez; Project Coordinator:Julian Ma
Phytoapps: Design of synthetic orthogonal regulatory gene circuits in plants using CRISPR-Cas9-like programmable DNA-binding proteins. (2017 – 2019)
IP: Dr. Diego Orzaez, Prof Antonio Granell
TomGEM: A holistic multi-actor approach towards the design of new tomato varieties and management practices to improve yield and quality in the face of climate change. (2016-2019)
IP: Prof Antonio Granell; Project Coordinator: Prof. Mondher Bouzayen
TRADITOM (634561). Traditional tomato varieties and cultural practicies: a case for agricultural diversification with impact on food security and health of European population. (2015 – 2018)
IP: Prof Antonio Granell (Project Coordinator)
Green switches: Design of synthetic gene circuits for recombinant protein production and nutritional enhancement in Solanaceous plants. (2014 – 2016)
IP: Dr. Diego Orzaez, Prof Antonio Granell
Production of recombinant polyclonal antibodies in Nicotiana benthamiana plants in an experimental greenhouse. (2014)
IP: Dr. Diego Orzaez
Production of Therapeutic Proteins in Plant Biofactories – PROTEBIOV. (2011 – 2014)
IP CSIC: Dr Diego Orzaez. Project Coordinator: IDEN BIOTECHNOLOGY S.L.
Engineering Tomatoes for Health: Building Blocks for Intragenesis and Molecular Farming in Solanaceae. (2011- 2013)
IP: Dr. Diego Orzaez
Nuevos productos cosméticos y sanitarios mediante producción recombinante. (2015 – 2017)
