117) Ortolá B. and J.A. Daròs (2020). Production of recombinant RNA in Escherichia coli using eggplant latent viroid as a scaffold. Methods in Molecular Biology (Viroids Methods and Protocols), in press

116) Daròs J.A. (2020). Two-dimensional polyacrylamide-gel electrophoresis analysis of viroid RNAs. Methods in Molecular Biology (Viroids Methods and Protocols), in press

115) Daròs J.A. (2020). Use of potyvirus vectors to produce carotenoids in plants. Methods in Molecular Biology (Plant and Food Carotenoids), 2083: 303-312, DOI: 10.1007/978-1-4939-9952-1_23

114) Carbonell A., P. Lisón and J.A. Daròs (2019). Multi-targeting of viral RNAs with synthetic trans-acting small interfering RNAs enhances plant antiviral resistance. Plant Journal, DOI: 10.1111/tpj.14466

113) Cervera-Seco L., M.C. Marques, A. Sanz-Carbonell, J. Marquez-Molins, A. Carbonell, J.A. Daròs and G. Gomez (2019). Identification and characterization of a stress-responsive TAS3-derived tasiRNAs in melon. Plant & Cell Physiology, DOI: 10.1093/pcp/pcz131

112) Pérez-de-Castro A., C. Esteras, A. Alfaro-Fernández, J.A. Daròs, A.J. Monforte, B. Picó and M.L. Gómez-Guillamón (2019). Fine mapping of wmv1551, a resistance gene to Watermelon mosaic virus in melon. Molecular Breeding, 39 (7): 93, DOI: 10.1007/s11032-019-0998-z

111) Shi X., T. Cordero, S. Garrigues, J.F. Marcos, J.A. Daròs and M. Coca  (2019). Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Plant Biotechnology Journal, 17 (6): 1069-1080, DOI: 10.1111/pbi.13038

110) Pasin F., W. Menzel and J.A. Daròs (2019). Harnessed viruses in the age of metagenomics and synthetic biology: an update on infectious clone assembly and biotechnologies of plant viruses. Plant Biotechnology Journal, 17 (6): 1010-1026, DOI: doi: 10.1111/pbi.13084

109) Aragonés V., A. Pérez-de-Castro, T. Cordero, J. Cebolla-Cornejo, C. López, B. Picó and J.A. Daròs (2019). A Watermelon mosaic virus clone tagged with the yellow visual maker phytoene synthase facilitates scoring infectivity in melon breeding programs. European Journal of Plant Pathology, 153: 317-323, DOI: 10.1007/s10658-018-01621-x

108) Di Serio F., E.M. Torchetti, J.A. Daròs and B. Navarro (2019). Reassessment of viroid RNA cytosine methylation status at the single nucleotide level. Viruses, 11 (4): E357, DOI: 10.3390/v11040357

107) Gobatto D., L.A. de Oliveira, D.A. de Siqueira Franco, N. Velásquez, J.A. Daròs and M. Eiras (2019). Surveys in the chrysanthemum production areas of Brazil and Colombia reveal that weeds are potential reservoirs of chrysanthemum stunt viroid. Viruses, 11 (4): E355, DOI: 10.3390/v11040355

106) Daròs J.A. (2019). ¿Pueden transformarse los virus de plantas en herramientas útiles para la biotecnología? Revista Alimentaria, 501: 90-92

105) Carbonell A., C. López and  J.A. Daròs (2019). Fast-forward identification of highly effective artificial small RNAs against different tomato spotted wilt virus isolates. Molecular Plant-Microbe Interactions, DOI: 10.1094/MPMI-05-18-0117-TA

104) Carbonell A. and J.A. Daròs (2019). Design, synthesis, and functional analysis of highly specific artificial small RNAs with antiviral activity in plants. Methods in Molecular Biology, 2028: 231-246, DOI: 10.1007/978-1-4939-9635-3_13

103) Cordero T., V. Aragonés and  J.A. Daròs (2018). Large-scale production of recombinant RNAs on a circular scaffold using a viroid-derived system in Escherichia coli. Journal of Visualized Expriments, 141: e58472, DOI: 10.3791/58472

102) Cordero T., A. Rosado, E. Majer, A. Jaramillo, G. Rodrigo and  J.A. Daròs (2018). Boolean computation in plants using post-translational genetic control and a visual output signal. ACS Synthetic Biology, 7 (10): 2322-2330, DOI: 10.1021/acssynbio.8b00214

101) Cordero T., B. Ortolá and  J.A. Daròs (2018). Mutational analysis of Eggplant latent viroid RNA circularization by the eggplant tRNA ligase in Escherichia coli. Frontiers in Microbiology 9: 635, DOI: 10.3389/fmicb.2018.00635

100) Daròs J.A., V. Aragonés and T. Cordero (2018). A viroid-derived system to produce large amounts of recombinant RNA in Escherichia coli. Scientific Reports 8 (1): 1904, DOI: 10.1038/s41598-018-20314-3

99) Daròs J.A. (2017). Eggplant latent viroid. In Viroids and satellites (editors A. Hadidi, R. Flores, J.W. Randles and P. Palukaitis), Oxford: Academic Press, pp. 339-344. ISBN: 978-0-12-801498-1, Elsevier Inc.

98) Rodrigo G., S. Prakash, S. Shen, E. Majer, J.A. Daròs and A. Jaramillo (2017). Model-based design of RNA hybridization networks implemented in living cells. Nucleic Acids Research 45 (16): 9797-9808, DOI: 10.1093/nar/gkx698

97) Daròs J.A. (2017). Viral suppressors: combating RNA silencing. Nature Plants 3: 17098, DOI: 10.1038/nplants.2017.98

96) Abdel-Hafez S.I.I., N.A. Nafady, I.R. Abdel-Rahim, A.M. Shaltout, J.A. Daròs and M.A. Mohamed (2017). Biosynthesis of silver nanoparticles using the compound curvularin isolated from the endophytic fungus Epicoccum nigrum: characterization and antifungal activity. Journal of Pharmaceutical and Applied Chemistry 3 (2): 135-146, DOI: 10.18576/jpac/030207

95) Carbonell A. and J.A. Daròs (2017). Artificial microRNAs and synthetic trans-acting small interfering RNAs interfere with viroid infection. Molecular Plant Pathology 18 (5): 746-753, DOI: 10.1111/mpp.12529

94) López-González S., V. Aragonés, J.A. Daròs, F. Sánchez and F. Ponz (2017). An infectious cDNA clone of a radish-infecting Turnip mosaic virus strain. European Journal of Plant Pathology 148: 207-211, DOI: 10.1007/s10658-016-1057-9

93) Cordero T., M.A. Mohamed, J.J. López-Moya and J.A. Daròs (2017). A recombinant Potato virus Y infectious clone tagged with the Rosea1 visual marker (PVY-Ros1) facilitates the analysis of viral infectivity and allows the production of large amounts of anthocyanins in plants. Frontiers in Microbiology 8: 611, DOI: 10.3389/fmicb.2017.00611

92) Rodrigo G., J.A. Daròs and S.F. Elena (2017). Virus-host interactome: putting the accent on how it changes. Journal of Proteomics 156: 1-4, DOI: 10.1016/j.jprot.2016.12.007

91) Majer E., B. Llorente, M. Rodríguez-Concepción and J.A. Daròs (2017). Rewiring carotenoid biosynthesis in plants using a viral vector. Scientific Reports 7: 41645, DOI: 10.1038/srep41645

90) Cordero T., Cerdán L., Carbonell A., Katsarou K., Kalantidis K. and J.A. Daròs (2017). Dicer-like 4 is involved in restricting the systemic movement of Zucchini yellow mosaic virus in Nicotiana benthamiana. Molecular Plant-Microbe Interactions 30 (1): 63-71, DOI: 10.1094/MPMI-11-16-0239-R

89) Daròs J.A. (2016). Eggplant latent viroid: a friendly experimental system in the family Avsunviroidae. Molecular Plant Pathology 17 (8):1170-1177,  DOI: 10.1111/mpp.12358

88) Daròs, J.A. (2016). Viroids: small noncoding infectious RNAs with the remakable ability of autonomous replication. En Current Research Topics in Plant Virology (editores Aiming Wang and Xueping Zhou). Springer International Publishing Switzerland, pp. 295-322. ISBN: 978-3-319-32917-8, ISBN: 978-3-319-32919-2 (eBook), DOI: 10.1007/978-3-319-32919-2.

87) Abdel-Hafez S.I.I, N.A. Nafady, I.R. Abdel-RAhim, A.M. Shaltout, J.A. Daròs and M.A. Mohamed (2016). Assessment of protein silver nanoparticles toxicity against pathogenic Alternaria solani. 3 Biotech 6 (2): 199, DOI: 10.1007/s13205-016-0515-6

86) Willemsen A., M.P. Zwart, N. Tromas, E, Majer, J.A. Daròs and S.F. Elena (2016). Multiple barriers to the evolution of alternative gene orders in a positive-strand RNA virus. Genetics 202 (4): 1503-1521, DOI: 10.1534/genetics.115.185017

85) Martínez F., G. Rodrigo, V. Aragonés, M. Ruiz, I. Lodewijk, U. Fernández, S.F. Elena and J.A. Daròs (2016). Interaction network of tobacco etch potyvirus NIa protein with the host plant during infection. BMC Genomics 17: 87, DOI: 10.1186/s12864-016-2394-y

84) Carbonell A., J.C. Carrington and J.A. Daròs (2016). Fast-forward generation of effective artificial small RNAs for enhanced antiviral defense in plants. RNA & Disease 3 (1): e1130. DOI: 10.14800/rd.1130

83) Majer E., J.A. Navarro and J.A. Daròs (2015). A potyvirus vector efficiently targets recombinant proteins to chloroplasts, mitochondria and nuclei in plant cells when expressed at the amino terminus of the polyprotein. Biotechnology Journal 10 (11): 1792-1802, DOI: 10.1002/biot.201500042

82) Rodrigo G., E. Majer, S. Prakash, J.A. Daròs, A. Jaramillo and J.F. Poyatos (2015). Exploring the dynamics and mutational landscape of riboregulation with a minimal synthetic circuit in living cells. Biophysical Journal 109 (5): 1070-1076, DOI: 10.1016/j.bpj.2015.07.021

81) Debreczeni D.E., C. López,  J. Aramburu, J.A. Daròs, S. Soler, L. Galipienso, B.W. Falk and L. Rubio (2015). Complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain. Archives of Virology 160 (8): 2117-2123, DOI: 10.1007/s00705-015-2453-8

80) Shen S., G. Rodrigo, S. Prakash, E. Majer, T.E. Landrain, B. Kirov, J.A. Daròs and A. Jaramillo (2015). Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression. Nucleics Acids Research 43 (10): 5158-5170, DOI: 10.1093/nar/gkv287

79) Martínez F. and J.A. Daròs (2014). Tobacco etch virus protein P1 traffics to the nucleolus and associates with the host 60S ribosomal subunits during infection. Journal of Virology 88 (18): 10725-10737, DOI: 10.1128/JVI.00928-14

78) Majer E., Z. Salvador, M.P. Zwart, A. Willemsen, S.F. Elena and J.A. Daròs (2014). Relocation of the NIb gene in the tobacco etch potyvirus genome. Journal of Virology 88 (8): 4586-4590, DOI: 10.1128/JVI.03336-13

77) Gobatto D., A.L.R. Chaves, R. Harakava, J.M. Marque, J.A. Daròs and M. Eiras (2014). Chrysanthemum stunt viroid in Brazil: survey, identification, biological and molecular characterization and detection methods. Journal of Plant Pathology 96 (1): 111-119.

76) Zwart M.P., A. Willemsen A., J.A. Daròs, S.F. Elena (2014). Experimental evolution of pseudogenization and gene loss in a plant RNA virus. Molecular Biology Evolución 31 (1): 121-134, DOI: 10.1093/molbev/mst175

75) Majer E., J.A. Daròs and M.P. Zwart (2013). Stability and fitness impact of the visually discernible Rosea1 marker in the Tobacco etch virus genome. Viruses 5 (9): 2153-2168, DOI: 10.3390/v5092153

74) Rodrigo G., T.E. Landrain, E. Majer, J.A. Daròs and A. Jaramillo (2013). Full design automation of multi-state RNA devices to program gene expression using energy-based optimization. PLoS Computational Biology 9 (8): e1003172, DOI: 10.1371/journal.pcbi.1003172

73) Martínez F., S.F. Elena and J.A. Daròs (2013). Fate of artificial microRNA-mediated resistance to plant viruses in mixed infections. Phytopathology 103 (8): 870-876, DOI: 10.1094/PHYTO-09-12-0233-R

72) Lafforgue G., F. Martínez, Q.W. Niu, N.H. Chua, J.A. Daròs and S.F. Elena (2013). Improving the effectiveness of amiRNA-mediated resistance against Turnip mosaic virus by combining two amiRNAs or by targeting highly conserved viral genomic regions. Journal of Virology 87 (14): 8254-8256, DOI: 10.1128/JVI.00914-13

71) Contreras-Paredes C.A., L. Silva-Rosales, J.A. Daròs, N.D. Alejandri-Ramírez, T.D. Dinkova (2013). The absence of eukaryotic initiation factor eIF(iso)4E affects the systemic spread of a Tobacco etch virus isolate in Arabidopsis thaliana. Molecular Plant-Microbe Interactions 26 (4): 461-470, DOI: 10.1094/MPMI-09-12-0225-R

70) Martínez F., G. Lafforgue G, M.J. Morelli, F. González-Candelas, N.H. Chua, J.A. Daròs and S.F. Elena (2012). Ultra-deep sequencing analysis of population dynamics of virus escape mutants in RNAi-mediated resistant plants. Molecular Biology Evolution 29 (11): 3297-3307, DOI: 10.1093/molbev/mss135

69) Zwart M., J.A. Daròs and S.F. Elena (2012). Effects of potyvirus effective population size in inoculated leaves on viral accumulation and the onset of symptoms. Journal of Virology 86 (18): 9737-9747, DOI: 10.1128/JVI.00909-12

68) Nohales M.A., R. Flores and J.A. Daròs (2012). Viroid RNA redirects host DNA ligase 1 to act as an RNA ligase. Proceedings of the National Academy of Sciences of the USA 109 (34): 13805-13810, DOI: 10.1073/pnas.1206187109

67) Nohales M.A., D. Molina-Serrano, R. Flores and J.A. Daròs (2012). Involvement of the chloroplastic isoform of tRNA ligase in the replication of viroids belonging to the family Avsunviroidae. Journal of Virology 86 (15): 8269-8276, DOI: 10.1128/JVI.00629-12

66) Sardanyés, J., F. Martínez, J.A. Daròs and S.F. Elena (2012). Dynamics of alternative modes of RNA replication for positive-sense RNA viruses. Journal of the Royal Society Interface 9 (69): 768-776, DOI: 10.1098/rsif.2011.0471.

65) Molina-Serrano D., J. Marqués J, M.A. Nohales, R. Flores and J.A. Daròs (2012). A chloroplastic RNA ligase activity analogous to the bacterial and archaeal 2’-5’ RNA ligase. RNA Biology 9 (3): 326-333, DOI: 10.4161/rna.19218

64) Marqués J., N. Duran-Vila, A. Namsi, J.M. Bové and J.A. Daròs (2012). Bacteria associated with the rhizosphere of manganese-deficient date palms affected by brittle leaf disease. Journal of Plant Pathology 94 (1): 157-169.

63) Bedoya L.C., F. Martínez, D. Orzáez and J.A. Daròs (2012). Visual tracking of plant virus infection and movement using a reporter MYB transcription factor that activates anthocyanin biosynthesis. Plant Physiology 158 (3): 1130-1138, DOI: 10.1104/pp.111.192922

62) De la Iglesia F., F. Martínez, J. Hillung, J.M. Cuevas, P.J. Gerrish, J.A. Daròs and S.F. Elena (2012). Luria-Delbrück estimation of turnip mosaic virus mutation rate in vivo. Journal of Virology 86 (6): 3386-3388, DOI: 10.1128/JVI.06909-11

61) Lafforgue, G., F. Martínez, J. Sardanyés, F. de la Iglesia, Q.W. Niu, S.S. Lin, R.V. Solé, N.H. Chua, J.A. Daròs and S.F. Elena (2011). Tempo and mode of plant RNA virus escape from RNA interference-mediated resistance. Journal of Virology 85 (19): 9686-9695, DOI: 10.1128/JVI.05326-11

60) Zwart, M., J.A. Daròs and S.F. Elena (2011). One is enough: in vivo effective population size is dose-dependent for a plant RNA virus. PLoS Pathogens 7 (7): e1002122, DOI: 10.1371/journal.ppat.1002122

59) Martínez, F., J. Sardanyés, S.F. Elena and J.A. Daròs (2011). Dynamics of a plant RNA virus intracellular accumulation: stamping machine versus geometric replication. Genetics 188 (3): 637-646, DOI: 10.1534/genetics.111.129114

58) Marqués, J., N. Duran-Vila and J.A. Daròs (2011). The Mn-binding proteins of the photosystem II oxygen-evolving complex are decreased in date palms affected by brittle leaf disease. Plant Physiology and Biochemistry 49 (4): 388-394, DOI: 10.1016/j.plaphy.2011.02.008

57) Eiras, M., M.A. Nohales, E.W. Kitajima, R. Flores and J.A. Daròs (2011). Ribosomal protein L5 and transcription factor IIIA from Arabidopsis thaliana bind in vitro specifically Potato spindle tuber viroid RNA. Archives of Virology 156 (3): 529-533, DOI: 10.1007/s00705-010-0867-x

56) Flores, R., J.A. Daròs, C. Hernández, F. Di Serio (2011). Viroids. Encyclopedia of Life Sciences, DOI: 10.1002/9780470015902.a0000434.pub3.

55) Bedoya, L.C., F. Martínez, L. Rubio and J.A. Daròs (2010). Simultaneous equimolar expression of multiple proteins in plants from a dissarmed potyvirus vector. Journal of Biotechnology, 150 (2): 268-275, DOI: 10.1016/j.jbiotec.2010.08.006

54) Eiras, M., S.R. Silva, E.S. Stuchi, R. Flores and J.A. Daròs (2010). Viroid species associated with the bark-cracking phenotype of ‘Tahiti’ acid lime in the State of São Paulo, Brazill. Tropical Plant Pathology 35 (5): 303-309.

53) Bedoya, L.C. and J.A. Daròs (2010). Stability of Tobacco etch virus infectious clones in plasmid vectors. Virus Research 149 (2): 234-240. DOI: 10.1016/j.virusres.2010.02.004

52) Torres-Barceló, C., J.A. Daròs and S.F. Elena (2010). Compensatory molecular evolution of HC-Pro, an RNA-silencing suppressor from a plant RNA virus. Molecular Biology and Evolution 27 (3): 543-551, DOI: 10.1093/molbev/msp272

51) Torres-Barceló, C., J.A. Daròs and S.F. Elena (2010). HC-Pro hypo- and hypersuppressor mutants: differences in viral siRNA accumulation in vivo and siRNA binding activity in vitro. Archives of Virology 155 (2): 251-254, DOI: 10.1007/s00705-009-0563-x

50) Martínez, F., J. Marqués, M.L. Salvador and J.A. Daròs (2009). Mutational analysis of Eggplant latent viroid RNA processing in Chlamydomonas reinhardtii chloroplast. Journal of General Virology 90 (12): 3057-3065, DOI: 10.1099/vir.0.013425-0

49) Elena, S.F., G. Gómez and J.A. Daròs (2009). Evolutionary constraints to viroid evolution. Viruses, 1 (2): 241-254, DOI: 10.3390/v1020241

48) Flores, R., M.E. Gas, D. Molina-Serrano, M.A. Nohales, A. Carbonell, S. Gago, M. de la Peña and J.A. Daròs (2009). Viroid replication: Rolling-circles, enzymes and ribozymes. Viruses, 1 (2): 317-334, DOI: 10.3390/v1020317

47) Serra, P., M. Eiras, S.M. Bani-Hashemian, N. Murcia, E.W. Kitajima, J.A. Daròs, R. Flores and N. Duran-Vila (2008). Citrus viroid V: occurrence, host range, diagnosis, and identification of new variants. Phytopathology, 98 (11): 1199-1204, DOI: 10.1094/PHYTO-98-11-1199

46) Torres-Barceló, C., S. Martín, J.A. Daròs and S.F. Elena (2008). From hypo- to hypersuppression: effect of amino acid substitutions on the RNA-silencing suppressor activity of the Tobacco etch potyvirus HC-Pro. Genetics, 180 (2): 1039-1049, DOI: 10.1534/genetics.108.091363

45) Gas, M.E., D. Molina-Serrano, C. Hernández, R. Flores and J.A. Daròs (2008). Monomeric linear RNA of Citrus exocortis viroid resulting from processing in vivo has 5′-phosphomonoester and 3′-hydroxyl termini: implications for the RNase and RNA ligase involved in replication. Journal of Virology, 82 (20): 10321-10325, DOI: 10.1128/JVI.01229-08

44) Duran-Vila, N., S.F. Elena, J.A. Daròs and R. Flores (2008). Structure and evolution of viroids. In Origin and evolution of viruses (2nd edition), E. Domingo, C.R. Parrish and J. Holland (eds.) pp. 43-64. Elsevier. ISBN: 978-0-12-374153.

43) Marqués, J., Z.G.N. Fadda, N. Duran-Vila, R. Flores, J.M. Bové and J.A. Daròs (2008). A set of novel RNAs transcribed from the chloroplast genome accumulates in date palm leaflets affected by brittle leaf disease. Phytopathology, 98 (3): 337-344, DOI: 10.1094/PHYTO-98-3-0337

42) Serra, P., C.J. Barbosa, J.A. Daròs, R. Flores and N. Duran-Vila (2008). Citrus viroid V: molecular characterization and synergistic interactions with other members of the genus Apscaviroid. Virology, 370 (1): 102-112, DOI: 10.1016/j.virol.2007.07.033

41) Flores, R., M.E. Gas, D. Molina, C. Hernández and J.A. Daròs (2008). Analysis of viroid replication. Methods in Molecular Biology, 451: 167-183.

40) Gas, M.E., C. Hernández, R. Flores and J.A. Daròs (2007). Processing of nuclear viroids in vivo: an interplay between RNA conformations. PLoS Pathogens, 3 (11): 1813-1826, DOI: 10.1371/journal.ppat.0030182

39) Martin, R., C. Arenas, J.A. Daròs, A. Covarrubias, J.L. Reyes and N.H. Chua (2007). Characterization of small RNAs derived from Citrus exocortis viroid (CEVd) in infected tomato plants. Virology, 367 (1): 135-146,       DOI: 10.1016/j.virol.2007.05.011

38) Sanjuán, R. and J.A. Daròs (2007). One-step site-directed mutagenesis of viroid dimeric cDNA. Journal of Virological Methods, 145 (1): 71-75, DOI: 10.1016/j.jviromet.2007.05.027

37) Eiras, M., E.W. Kitajima, R. Flores and J.A. Daròs (2007). Existence in vivo of the loop E motif in potato spindle tuber viroid RNA. Archives of Virology, 152 (7): 1389-1393, DOI: 10.1007/s00705-007-0952-y

36) Molina-Serrano, D., L. Suay, M.L. Salvador, R. Flores and J.A. Daròs (2007). Processing of RNAs of the family Avsunviroidae in Chlamydomonas reinhardtii chloroplasts. Journal of Virology, 81 (8): 4363-4366, DOI: 10.1128/JVI.02556-06

35) Carrasco, P., J.A. Daròs, P. Agudelo-Romero and S.F. Elena (2007). A real-time RT-PCR assay for quantifying the fitness of tobacco etch virus in competition experiments. Journal of Virological Methods, 139 (2): 181-188, DOI: 10.1016/j.jviromet.2006.09.020

34) Namsi, A., J. Marqués, Z. Fadda, M.L. Takrouni, O. Ben Mahamoud, A. Zouba, J.A. Daròs, R. Flores, J.M. Bové and N. Duran-Vila (2006). Diagnosis of “maladie des feuilles cassantes” or brittle leaf disease of date palms by detection of associated chloroplast encoded double stranded RNAs. Molecular and Cellular Probes, 20 (6): 366-370, DOI: 10.1016/j.mcp.2006.05.001

33) Codoñer, F.M., J.A. Daròs, R.V. Solé and S.F. Elena (2006). The fittest versus the flattest: experimental confirmation of the quasispecies effect with subviral pathogens. PLoS Pathogens, 2 (12): 1187-1193, DOI: 10.1371/journal.ppat.0020136

32) Eiras, M., J.A. Daròs, R. Flores and E.W. Kitajima (2006). Viróides e virusóides: relíquias do mundo de RNA. Fitopatologia Brasileira, 31 (3): 229-246.

31) Hernández, C., F. Di Serio, S. Ambrós, J.A. Daròs and R. Flores (2006). An element of the tertiary structure of peach latent mosaic viroid RNA revealed by UV irradiation. Journal of Virology, 80 (18): 9336-9340, DOI: 10.1128/JVI.00630-06

30) Di Serio, F., J.A. Daròs, A. Ragozzino and R. Flores (2006). Close structural relationship between two hammerhead viroid-like RNAs associated with cherry chlorotic rusty spot disease. Archives of Virology, 151 (8): 1539-1549, DOI: 10.1007/s00705-006-0732-0

29) Daròs, J.A., S.F. Elena and R. Flores (2006). Viroids: an Ariadne’s thread into the RNA labyrinth. EMBO Reports, 7 (6): 593-598, DOI: 10.1038/sj.embor.7400706

28) Elena, S.F., P. Carrasco, J.A. Daròs and R. Sanjuán (2006). Mechanisms of genetic robustness in RNA viruses. EMBO Reports, 7 (2): 168-173, DOI: 10.1038/sj.embor.7400636

27) Flores, R., J.A. Daròs, C. Hernández and F. Di Serio (2005). Viroids. En Encyclopedia of Life Sciences, S. Robertson (ed.). Macmillan Reference Limited (Nature Publishing Group), London, U.K. www.els.net

26) Flores, R., C. Hernández, A.E. Martínez de Alba, J.A. Daròs and F. Di Serio (2005). Viroids and viroid-host interactions. Annual Reviews in Phytopathology, 43: 117-139,      DOI: 10.1146/annurev.phyto.43.040204.140243

25) Daròs, J.A. and R. Flores (2004). Arabidopsis thaliana has the enzymatic machinery for replicating representative viroid species of the family Pospiviroidae. Proceedings of the National Academy of Sciences of the USA, 101 (17):6792-6797, DOI: 10.1073/pnas.0401090101

24) Fadda, Z., J.A. Daròs, R. Flores and N. Durán-Vila (2003). Identification in eggplant of a variant of citrus exocortis viroid (CEVd) with a 96 nucleotide duplication in the right terminal region of the rod-like secondary structure. Virus Research, 97 (2):145-149, DOI: 10.1016/j.virusres.2003.08.002

23) Triki, M.A., A. Zouba, O. Khoualdia, O. Ben Mahamoud, M.L. Takrouni, M. Garnier, J.M. Bové, M. Montarone, A. Poupet, R. Flores, J.A. Daròs, Z.G.N. Fadda, P. Moreno and N. Durán-Vila (2003). “Maladie des feuilles cassantes” or brittle leaf disease of date palms in Tunisia: biotic or abiotic disease? Journal of Plant Pathology, 85 (2): 71-79.

22) Fadda, Z., J.A. Daròs, C. Fagoaga, R. Flores and N. Durán-Vila (2003). Eggplant latent viroid, the candidate type species for a new genus within the family Avsunviroidae (hammerhead viroids). Journal of Virology, 77 (11): 6528-6532, DOI: 10.1128/JVI.77.11.6528-6532.2003

21) Flores, R., J.A. Daròs and J.A. Navarro (2003). Replication. In Viroids, A. Hadidi, R. Flores, J.W. Randles and J.S. Semancik (eds.) pp. 55-60. CSIRO Publishing, Collingwood VIC3066, Australia. ISBN: 0 643 06789 2.

20) Daròs, J.A. and R. Flores (2002). A chloroplast protein binds a viroid RNA in vivo and facilitates its hammerhead-mediated self-cleavage. EMBO Journal, 21 (4): 749-759, DOI: 10.1093/emboj/21.4.749

19) Flores, R., C. Hernández, M. de la Peña, A. Vera and J.A. Daròs (2001). Hammerhead ribozyme structure and function in plant RNA replication. Methods in Enzymology, 341: 540-552.

18) Flores, R., J.A. Daròs, C. Hernández and F. Di Serio (2001). Viroids. En Encyclopedia of Life Sciences, S. Robertson (ed.). Macmillan Reference Limited (Nature Publishing Group), London, U.K. www.els.net

17) Vera, A., J.A. Daròs, R. Flores and C. Hernández (2000). The DNA of a plant retroviroid-like element is fused to different sites in the genome of a plant pararetrovirus and shows multiple forms with sequence deletions. Journal of Virology, 74 (22): 10390-10400, DOI: 10.1128/JVI.74.22.10390-10400.2000

16) Flores, R., J.A. Daròs and C. Hernández (2000). Avsunviroidae family: viroids containing hammerhead ribozymes. Advances in Virus Research, 55: 271-323,       DOI: 10.1016/S0065-3527(00)55006-4

15) Daròs, J.A., M. Schaad and J.C. Carrington (1999). Functional analysis of the interaction between VPg-proteinase (NIa) and RNA polymerase (NIb) of tobacco etch potyvirus, using conditional and suppressor mutants. Journal of Virology, 73 (10): 8732-8740.

14) Navarro, J.A., J.A. Daròs and R. Flores (1999). Complexes containing both polarity strands of avocado sunblotch viroid: identification in chloroplasts and characterization. Virology, 253 (1): 77-85, DOI: 10.1006/viro.1998.9497

13) Navarro, B., J.A. Daròs and R. Flores (1998). Reverse transcription polymerase chain reaction protocols for cloning small circular RNAs. Journal of Virological Methods, 73 (1): 1-9, DOI: 10.1016/S0166-0934(98)00042-1

12) Haldeman-Cahill, R., J.A. Daròs and J.C. Carrington (1998). Secondary structures in the capsid protein coding sequence and 3′ nontranslated region involved in amplification of the tobacco etch virus genome. Journal of Virology, 72 (5): 4072-4079.

11) Daròs, J.A. and J.C. Carrington (1997). RNA binding activity of NIa proteinase of tobacco etch potyvirus. Virology, 237 (2): 327-336, DOI: 10.1006/viro.1997.8802

10) Di Serio, F., J.A. Daròs, A. Ragozzino and R. Flores (1997). A 451-nucleotide circular RNA from cherry with hammerhead ribozymes in its strands of both polarities. Journal of Virology, 71 (9): 6603-6610.

9) Daròs, J.A. and R. Flores (1996). A group I plant intron accumulates as circular RNA forms with extensive 5’ deletions in vivo. RNA, 2 (9): 928-936.

8) Navarro, B., J.A. Daròs and R. Flores (1996). A general strategy for cloning viroids and other small circular RNAs that uses minimal amounts of template and does not require prior knowledge of its sequence. Journal of Virological Methods, 56 (1): 59-66, DOI: 10.1016/0166-0934(95)01912-X

7) Daròs, J.A. y R. Flores (1995). Viroides y otros pequeños RNAs circulares e infecciosos de animales y plantas. En Microbiología y Genética Molecular, Josep Casadesús (ed.) Servicio de Publicaciones de la Universidad de Huelva. ISBN: 84-88751-24-9.

6) Daròs, J.A. and R. Flores (1995). Characterization of multiple circular RNAs derived from a plant viroid-like RNA by sequence deletions and duplications. RNA, 1 (7): 734-744.

5) Daròs, J.A. and R. Flores (1995). Identification of a retroviroid-like element from plants. Proceedings of the National Academy of Sciences of the USA, 92 (15): 6856-6860, DOI: 10.1073/pnas.92.15.6856

4) Daròs, J.A., J.F. Marcos, C. Hernández and R. Flores (1994). Replication of avocado sunblotch viroid: evidence for a symmetric pathway with two rolling circles and hammerhead ribozyme processing. Proceedings of the National Academy of Sciences of the USA, 91 (26): 12813-12817, DOI: 10.1073/pnas.91.26.12813

3) Hernández, C., J.A. Daròs, S.F. Elena, A. Moya and R. Flores (1992). The strands of both polarities of a small circular RNA from carnation self-cleave in vitro through alternative double- and single-hammerhead structures. Nucleic Acids Research, 20 (23): 6323-6329, DOI: 10.1093/nar/20.23.6323

2) Braco, L., J.A. Daròs and M. de la Guardia (1992). Enzymatic flow injection analysis in nonaqueous media. Analytical Chemistry, 64 (2): 129-133, DOI: 10.1021/ac00026a006

1) Rández-Gil, F., J.A. Daròs, A. Salvador and M. de la Guardia (1991). Direct derivative spectrophotometric determination of nitrazepam and clonazepam in biological fluids. Journal of Pharmaceutical and Biomedical Analysis, 9 (7): 539-545, DOI: 10.1016/0731-7085(91)80175-9