El principal objetivo es desarrollar y aplicar distintas alternativas biotecnológicas para la mejora genética de especies hortícolas (melón, sandía, pepino y tomate) y plantas ornamentales. Las técnicas que manejamos para ello son:
- Transformación genética de hortícolas para conseguir aumentar la tolerancia a la salinidad y estrés hídrico, la resistencia a enfermedades, la modificación genética de caracteres del desarrollo y la mejora de la calidad
- Transformación genética de especies ornamentales para conseguir el retraso de la senescencia y la modificación genética de caracteres del desarrollo
- Genómica funcional mediante mutagénesis insercional, trapping, PTGS,…
- Fusión de protoplastos para la obtención de nuevos aloploides y de híbridos asimétricos
- Obtención de haploides y dobles-haploides de especies hortícolas
- Selección somaclonal de plantas ornamentales
- Micropropagación de especies carnívoras y ornamentales
Especies hortícolas: Tomate (Solanum lycopersicum), Melón (Cucumis melo L.), Sandía (Citrullus lanatus Thunb [Matsum & Nakai], Pepino (Cucumis sativus L.).
Especies silvestres relacionadas de los géneros: Solanum sp., Cucumis sp. y Citrullus sp.
Plantas ornamentales: Ficus benjamina, F. lyrata, F. elastica, Codiaeum variegatum, Kalanchoe blossfeldiana, Begonia sp., Phylodendron sp., Spatiphyllum sp., Syngonium sp., Columnea sp., Schlumbergera sp., Hatiora sp., Pelargonium x hortorum, Pelargonium peltatum y Pelargonium domesticum
Plantas carnívoras: Dionaea muscipula, Drosera aliciae, D. binata, D. capensis tipica, D. capensis alba, D. nidiformis, D. spatulata, D. regia, D. nidiformis, Drosophyllum lusitanicum, Byblis liniflora, Sarracenia x moorei, Darlingtonia californica,…
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Pérez-Martín, F; Yuste-Lisbona, F; Pineda, B; García-Sogo, B; del Olmo, I ; Alché, JD; Egea, I; Flores, F; Pineiro, M; Jarillo, J; Angosto, T; Capel, J; Moreno, V; Lozano, R. (2018)
Developmental role of the tomato mediator complex subunit MED18 in pollen ontogeny.
Plant Journal 96: 300 – 315
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Egea I, Pineda B, Ortíz-Atienza A, Plasencia F, Drevensek S, García-Sogo B, Yuste-Lisbona F, Barrero J, Atarés A, Flores F, Barneche F, Angosto T, Capel C, Salinas J, Vriezen W, Esch E, Bowler C, Bolarín MC, Moreno V, Lozano R (2018)
Tomato SlCBL10 ensures plant growth under salt stress by regulating Na+ and Ca2+ homeostasis
Plant Physiology, 176: 1676 – 1693
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Pérez-Martín F, Yuste-Lisbona F, Pineda B, Angarita-Díaz M, García-Sogo B, Antón T, Sánchez S, Giménez-Caminero E, Atarés A, Fernández-Lozano A, Ortíz-Atienza A, García-Alcázar M, Castañeda L, Fonseca R, Capel C, Goergen G, Sánchez J, Quispe J, Capel J, Angosto T, Moreno V, Lozano R (2017)
A collection of enhancer trap insertional mutants for functional genomics in tomato
Plant Biotechnology Journal 15, 1439–1452
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García-Alcázar M, Giménez E, Pineda B, Capel C, García-Sogo B, Sánchez S, Yuste-Lisbona FJ, Angosto T, Capel J, Moreno V, Lozano R (2017)
Albino T-DNA tomato mutant reveals a key function of 1-deoxy-D-xylulose-5-phosphate synthase (DXS1) in plant development and survival
Scientific Reports 7: 45333
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Jaime-Pérez N, Pineda B, Garcia-Sogo B, Atares A, Athman A, Byrt CS, Olias R, Asins MJ, Gilliham M, Moreno V, Belver A. (2017)
The Na+ transporter encoded by the HKT1;2 gene modulates Na+/K+ homeostasis in tomato shoots under salinity
Plant, Cell & Environment 40: 658-671
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Rojas-Gracia P, Roque E, Medina M, Rochina M, Hamza R, Angarita-Díaz MP; Moreno V, Pérez-Martín F; Lozano R, Cañas L, Beltrán JP, Gómez-Mena C (2017)
The parthenocarpic hydra mutant reveals a new function for a SPOROCYTELESS-like gene in the control of fruit set in tomato
New Phytologist 214 (3) 1198-1212
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Yuste-Lisbona FJ, Quinet M, Fernández-Lozano A, Pineda B, Moreno V, Angosto T, Lozano R (2016)
Characterization of vegetative inflorescence (mc-vin) mutant provides new insight into the role of MACROCALYX in regulating inflorescence development of tomato
Scientific Reports 6: 18796
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Yuste-Lisbona FJ, Quinet M, Fernández-Lozano A, Pineda B, Moreno V, Angosto T & Lozano R (2016)
Characterization of vegetative inflorescence (mc-vin) mutant provides new insight into the role of MACROCALYX in regulating inflorescence development of tomato
Scientific Reports 6: 18796
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Campos JF, Cara B, Pérez-Martín F, Pineda B, Egea I, Flores FB, Fernandez-Garcia N, Capel J, Moreno V, Angosto T, Lozano R, Bolarin MC (2016)
The tomato mutant ars1 (altered response to salt stress 1) identifies an R1-type MYB transcription factor involved in stomatal closure under salt acclimation
Plant Biotechnology Journal 14: 1345-1356
- Campos J, Cara B, Pérez-Martín F, Pineda B, Egea I, Flores F, Fernández Garcia N, Capel J, Moreno V, Angosto T, Lozano R, Bolarín MC (2016)
The tomato mutant ars1 (altered response to salt stress 1) identifies an R1-type MYB transcription factor involved in stomatal closure under salt acclimation
Plant Biotechnology Journal 14 (6): 1345-1356
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Gimenez E, Castañeda L, Pineda B, Pan IL, Moreno V, Angosto T, Lozano R (2016)
TOMATO AGAMOUS1 and ARLEQUIN/TOMATO AGAMOUS-LIKE1 MADS-box genes have redundant and divergent functions required for tomato reproductive development
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Gimenez E, Castañeda L, Pineda B, Pan, IL, Moreno V, Angosto T, Lozano R (2016)
TOMATO AGAMOUS1 and ARLEQUIN/TOMATO AGAMOUS-LIKE1 MADS-box genes have redundant and divergent functions required for tomato reproductive development.
Plant Molecular Biology. 91: 513–531
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Fernandez-Lozano A, Yuste-Lisbona FJ, Perez-Martin F, Pineda B, Moreno V, Lozano R, Angosto T (2015)
Mutation at the tomato EXCESSIVE NUMBER OF FLORAL ORGANS (ENO) locus impairs floral meristem development, thus promoting an increased number of floral organs and fruit size
Plant Science. 232: 41-48
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Fernandez-Lozano A, Yuste-Lisbona FJ, Perez-Martin F, Pineda B, Moreno V, Lozano R, Angosto T (2015)
Mutation at the tomato EXCESSIVE NUMBER of FLORAL ORGANS (ENO) locus impairs floral meristem development,thus promoting an increased number of floral organs and fruit size
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Garcia-Abellan J, Fernandez-Garcia N, Lopez-Berenguer C, Egea I, Flores F, Angosto T, Capel J, Lozano R, Pineda B, Moreno V, Olmos E, Bolarin, MC (2015)
The tomato res mutant which accumulates JA in roots in non-stressed conditions restores cell structure alterations under salinity
Physiologia Plantarum 155: 296–314
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Giménez E, Dominguez E, Pineda B, Heredia A, Moreno V, Lozano R, Angosto T (2015)
Transcriptional Activity of the MADS Box ARLEQUIN/TOMATO AGAMOUS-LIKE Gene Is Required for Cuticle Development of Tomato Fruit
Plant Physiology 168: 1036-1048
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Giménez E, Domínguez E, Pineda B, Heredia A, Moreno V, Lozano R, Angosto T (2015)
Transcriptional activity of the MADS Box ARLEQUIN/TOMATO AGAMOUS-LIKE1 gene is required for cuticle development of tomato fruit
Plant Physiology 168 (3): 1036-1048
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García-Abellan JO, Egea I, Pineda B, Sanchez-Bel P , Belver A, Garcia-Sogo B, Flores FB, Atares A, Moreno V, Bolarin MC (2014)
Heterologous expression of the yeast HAL5 gene in tomato enhances salt tolerance by reducing shoot Na+accumulation in the long term
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Molina MC, Lopez CG, Staltari S, Chorzempa SE, Moreno V (2013)
Cryptic homoeology analysis in species and hybrids of genus Zea
Biologia Plantarum 57: 449-456
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Juarez P, Presa S, Espi J, Pineda B, Anton MT, Moreno V, Buesa J, Granell A, Orzaez D (2012)
Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes
Plant Biotechnology Journal 10: 341-352
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Munoz-Mayor A, Pineda B, Garcia-Abellan JO, Anton T, Garcia-Sogo B, Sanchez-Bel P, Flores FB, Atares A, Angosto T, Pintor-Toro JA, Moreno V, Bolarin MC (2012)
Overexpression. of dehydrin tas14 gene improves the osmotic stress imposed by drought and salinity in tomato
Journal of Plant Physiology 169: 459-468
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Pineda B, Giménez E, García-Sogo B, Antón T, Atarés A, Capel J, Lozano R, Angosto T, Moreno V (2010)
Genetic and Physiological Characterization of the Arlequin Insertional Mutant Reveals a Key Regulator of Reproductive Development in Tomato
Plant Cell Physiology 51: 435-447
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Estornell LH, Orzaez D, Lopez-Pena L, Pineda B, Anton MT, Moreno V, Granell A (2009)
A multisite gateway-based toolkit for targeted gene expression and hairpin RNA silencing in tomato fruits
Plant Biotechnology Journal 7: 298-309
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Muñoz-Mayor,A, Pineda B, Garcia-Abellan J, Garcia-Sogo B, Moyano E, Atares A, Vicente-Agull F, Serrano R, Moreno V, Bolarin MC (2008)
The HAL1 function on Na+ homeostasis is maintained over time in salt-treated transgenic tomato plants, but the high reduction of Na+ in leaf is not associated with salt tolerance
Physiologia Plantarum 133: 288-297
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Cuartero J, Bolarín MC, Asíns MJ, Moreno V (2006)
Increasing salt tolerance in the tomato
Journal of Experimental Botany 57: 1045-1058
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Ellul P, Angosto T, García-Sogo B, García-Hurtado N, Zurita S, Martín-Trillo M, Martínez Zapater JM, Moreno V, Lozano R (2004)
Heterologous expression of Arabidopsis APETALA 1 gene in transgenic tomato influences plant architecture and flowering time
Molecular Breeding 13: 155-163
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Ellul P, Rios G, Atares A, Serrano R, Moreno V (2003)
The expressión of the Saccharomyces cerevisiae HAL1 gene increases salt tolerance in transgenic watermelon (Citrullus lanatus [Thunb] Matsun & Nakai)
Theoretical & Applied Genetics 107: 462-469
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Ellul P, García-Sogo B, Pineda B, Ríos G, Roig L, Moreno V (2003)
The ploidy level of transgenic plants in Agrobacterium-mediated transformation of tomato cotyledons (Lycopersicon esculentum Mill) is genotype and procedure dependent
Theoretical & Applied Genetics 106: 231-238
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Ellul P, Boscaiu M, Vicente O, Moreno V, Roselló JA (2002)
Intra- and interspecific variation in DNA content in Cistus (Cistacae)
Annals of Botany 90: 345-351
- Arrillaga I, Gisbert C, Sales E, Roig L, Moreno V (2001)
In vitro plant regeneration and gene transfer in the wild tomato Lycopersicon cheesmanii
Journal of Horticultural Science & Biotechnology 76: 413-418
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Gisbert C, Rus AM, Bolarin MC, Lopez-Coronado JM, Arrillaga I, Montesinos C, Caro M, Serrano R, Moreno V (2000)
The yeast HAL1 gene improves salt tolerance of transgenic tomato
Plant Physiology 123: 393-402
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Gisbert C, Arrillaga I, Roig LA, Moreno V (1999)
Acquisition of a collection of lycopersicon pennellii (Corr. D’arcy) transgenic plants with uidA and nptii marker genes
Journal of Horticultural Science & Biotechnology 74: 105-109
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Serrano R, Culianz-Macia FA, Moreno V (1999)
Genetic engineering of salt and drought tolerance with yeast regulatory genes
Scientia Horticulturae 78: 261-269
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Cano EA, Perez-Alfocea F, Moreno V, Caro M, Bolarin MC (1998)
Evaluation of salt tolerance in cultivated and wild tomato species through in vitro shoot apex culture
Plant Cell Tissue and Organ Culture 53: 19-26
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Arrillaga I, Gil-Mascarell R, Gisbert C, Sales E, Montesinos C, Serrano R, Moreno V (1998)
Expression of the yeast HAL2 gene in tomato increases the in vitro salt tolerance of transgenic progenies
Plant Science 136: 219-226
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Dabauza M, Gonzalez-Candelas L, Bordas M, Roig LA, Ramon D, Moreno V (1998)
Regeneration and characterisation of cucumis melo l. (+) cucumis anguria l. Var. Longipes (hook. Fil.) Meeuse somatic hybrids
Plant Cell Tissue and Organ Culture 52: 123-131
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Bordas M, Gonzalez-Candelas L, Dabauza M, Ramon D, Moreno V (1998)
Somatic hybridization between an albino cucumis melo l. Mutant and cucumis myriocarpus naud.
Plant Science 132: 179-190
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Bordas, M, Montesinos, C, Dabauza, M, Salvador A, Roig LA, Serrano R, Moreno V (1997)
Transfer of the yeast salt tolerance gene HAL1 to cucumis melo l cultivars and in vitro evaluation of salt tolerance
Transgenic Research 6: 41-50
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Dabauza M, Bordas M, Salvador A, Roig LA, Moreno V (1996)
Plant regeneration and agrobacterium-mediated transformation of cotyledon explants of citrullus colocynthis (l.) Schrad.
Plant Cell Reports 16: 888-892
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Cano EA, PerezAlfocea F, Moreno V, Bolarin MC (1996)
Responses to NaCl stress of cultivated and wild tomato species and their hybrids in callus cultures
Plant Cell Reports 15: 791-794
