GRUPO: Biotecnología de Virus de Plantas

Clave: L = libro completo, CL = capítulo de libro, A = artículo, R = “review”
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143) Talens-Perales D., M. Nicolau-Sanus, J. Polaina and J.A. Daròs (2022)
Expression of an extremophilic xylanase in Nicotiana benthamiana and its use for the production of prebiotic xylooligosaccharides
Scientific Reports, 12 (1): 15743, DOI: 10.1038/s41598-022-19774-5 Clave: A

142) Cisneros A.E., P. Lisón, L. Campos, J.M. López-Tubau, T. Altabella, A. Ferrer, J.A. Daròs and A. Carbonell (2022).
Downregulation of tomato STEROL GLYCOSYLTRANSFERASE 1 perturbs plant development and facilitates viroid infection
Journal of Experimental Botany, erac361, DOI: 10.1093/jxb/erac361 Clave: A

141) Selma S., S. Gianoglio, M. Uranga, M. Vázquez-Vilar, A. Espinosa-Ruiz, A. Drapal, P.D. Fraser, J.A. Daròs and D. Orzaez (2022)
Potato virus X-delivered CRISPR activation programs lead to strong endogenous gene induction and transient metabolic reprogramming in Nicotiana benthamiana
Plant Journal, 111 (6): 1550-1564, DOI: 10.1111/tpj.15906 Clave: A

140) Marqués M.C., J. Sánchez-Vicente, R. Ruiz, R. Montagud-Martínez, R. Márquez-Costa, G. Gómez, A. Carbonell, J.A. Daròs and G. Rodrigo (2022)
Diagnostics of infections produced by the plant viruses TMV, TEV, and PVX with CRISPR-Cas12 and CRISPR-Cas13
ACS Synthetic Biology, 11 (7): 2384-2393, DOI: 10.1021/acssynbio.2c00090 Clave: A

139) Aragonés V., F. Aliaga, F. Pasin and J.A. Daròs (2022)
Simplifying plant gene silencing and genome editing logistics by a one-Agrobacterium system for simultaneous delivery of multipartite virus vectors
Biotechnology Journal, 17 (7): e2100504, DOI: 10.1002/biot.202100504 Clave: A

138) Pasin F., J.A. Daròs and I.E. Tzanetakis (2022)
Proteome expansion in the Potyviridae evolutionary radiation
FEMS Microbiology Reviews, 46 (4): fuac011, DOI: 10.1093/femsre/fuac011 Clave: R

137) Uranga M. and J.A. Daròs (2022)
Tools and targets: the dual role of plant viruses in CRISPR-Cas genome editing
Plant Genome, e20220, DOI: 10.1002/tpg2.20220 Clave: R

136) Houhou F., M. Martí, T. Cordero, V. Aragonés, C. Sáez, J. Cebolla-Cornejo, A. Pérez de Castro A, M. Rodríguez-Concepción, B. Picó and J.A. Daròs (2022)
Carotenoid fortification of zucchini fruits using a viral RNA vector
Biotechnology Journal, 17 (5): e2100328, DOI: 10.1002/biot.202100328 Clave: A

135) Pallas V., C. Hernández, J.F. Marcos, J.A. Daròs, S. Ambrós, B. Navarro, J.A. Navarro, M. de la Peña, S. Gago-Zachert, M.E. Gas, A. Carbonell, C. Lopez, A.E. Martínez de Alba, F. Di Serio and P. Moreno (2022)
In Memoriam of Ricardo Flores: The Career, Achievements, and Legacy of an inspirational plant virologist
Virus Research, 312: 198718, DOI: 10.1016/j.virusres.2022.198718 Clave: R

134) Rodriguez-Concepcion M. and J.A. Daròs (2022)
Transient expression systems to rewire plant carotenoid metabolism
Current Opinion in Plant Biology, 66: 102190, DOI: 10.1016/j.pbi.2022.102190 Clave: R

133) Martí M., F. Merwaiss, A. Butkovic and J.A. Daròs (2022)
Production of potyvirus-derived nanoparticles decorated with a nanobody in biofactory plants
Frontiers in Bioengineering and Biotechnology, 10: 877363, DOI: 10.3389/fbioe.2022.877363
Clave: A

132) Calvache C., M. Vazquez-Vilar, S. Selma, M. Uranga, A. Fernández-del-Carmen, J.A. Daròs and D. Orzáez (2022)
Strong and tunable anti-CRISPR/Cas activities in plants
Plant Biotechnology Journal, 20 (2): 399-408, DOI: 10.1111/pbi.13723 Clave: A

131) López-Dolz L, M. Spada, J.A. Daròs and A. Carbonell (2022)
Fine-tuning plant gene expression with synthetic trans-acting small interfering RNAs
Methods in Molecular Biology (Plant Gene Silencing), 2408: 227-242, DOI: 10.1007/978-1-0716-1875-2_15 Clave: R

130) Ortolá B. and J.A. Daròs (2022)
Production of recombinant RNA in Escherichia coli using eggplant latent viroid as a scaffold
Methods in Molecular Biology (Viroids: Methods and Protocols), 2316: 315-327, DOI: 10.1007/978-1-0716-1464-8_25 Clave: R

129) Daròs J.A. (2022)
Two-dimensional polyacrylamide-gel electrophoresis analysis of viroid RNAs
Methods in Molecular Biology (Viroids: Methods and Protocols), 2316: 79-88, DOI: 10.1007/978-1-0716-1464-8_8 Clave: R

128) Ortolá B., T. Cordero, X. Hu and J.A. Daròs (2021)
Intron-assisted, viroid-based production of insecticidal circular double-stranded RNA in Escherichia coli
RNA Biology, 18 (11): 1846-1857, DOI: 10.1080/15476286.2021.1872962 Clave: A

127) Uranga M., M. Vazquez-Vilar, D. Orzáez and J.A. Daròs (2021)
CRISPR-Cas12a genome editing at the whole-plant level using two compatible RNA virus vectors
CRISPR Journal, 4 (5): 761-769, DOI: 10.1089/crispr.2021.0049 Clave: A

126) Ambrós S., N. Gómez-Muñoz, S. Giménez-Santamarina, J. Sánchez-Vicente, J. Navarro-López, F. Martínez, J.A. Daròs and G. Rodrigo (2021)
Molecular signatures of silencing suppression degeneracy from a complex RNA virus
PLoS Computational Biology, 17 (6): e1009166, DOI: 10.1111/pbi.13723 Clave: A

125) Montagud-Martínez R., M. Heras-Hernández, L. Goiriz, J.A. Daròs and G. Rodrigo (2021)
CRISPR-mediated strand displacement logic circuits with toehold-free DNA
ACS Synthetic Biology, 10 (5): 950-956, DOI: 10.1021/acssynbio.0c00649 Clave: A

124) Uranga M., V. Aragonés, S. Selma, M. Vázquez-Vilar, D. Orzáez and J.A. Daròs (2021)
Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector
Plant Journal, 106 (2): 555-565, DOI: 10.1111/tpj.15164 Clave: A

123) Daròs J.A. (2021)
Identification and functional characterization of viroid circular RNAs
Methods in Molecular Biology (Plant Circular RNAs: Methods and Protocols), 2362:101-107, DOI: 10.1007/978-1-0716-1645-1_6 Clave: R

122) Daròs J.A. (2021)
Production of circular recombinant RNA in Escherichia coli using viroid scaffolds
Methods in Molecular Biology (RNA Scaffolds: Methods and Protocols), 2323: 99-107, DOI: 10.1007/978-1-0716-1499-0_8 Clave: R

121) Llorente B., S. Torres-Montilla, L. Morelli, I. Florez-Sarasa, J.T. Matus, M. Ezquerro, L. D’Andrea, F. Houhou, E. Majer, B. Picó, J. Cebolla, A. Troncoso, A.R. Fernie, J.A. Daròs and M. Rodriguez-Concepcion (2020).
Synthetic conversion of leaf chloroplasts into carotenoid-rich plastids reveals mechanistic basis of natural chromoplast development.
Proceedings of the National Academy of Sciences of the USA 117 (35): 21796-21803, DOI: 10.1073/pnas.2004405117 Clave: A

120) Martí M., G. Diretto, V. Aragonés, S. Frusciante, O. Ahrazem, L. Gómez-Gómez and J.A. Daròs (2020).
Efficient production of saffron crocins and picrocrocin in Nicotiana benthamiana using a virus-driven system.
Metabolic Engineering 61: 238-250, DOI: 10.1016/j.ymben.2020.06.009 Clave: A

119) Flores R., J.A. Daròs, C. Hernández, B. Navarro, F. Di Serio (2020).
Viroids.
Encyclopedia of Life Science, 1: 192-203, DOI: 10.1002/9780470015902.a0029141 Clave: R

118) López-Dolz L., M. Spada, J.A. Daròs and A. Carbonell (2020).
Fine-tune control of targeted RNAi efficacy by plant artificial small RNAs.
Nucleic Acids Research 48 (11): 6234-6250, DOI: 10.1093/nar/gkaa343 Clave: A

117) El-Abeid S.E., Y. Ahmed, J.A. Daròs and M.A. Mohamed (2020).
Reduced graphene oxide nanosheet-decorated copper oxide nanoparticles: a potent antifungal nanocomposite against Fusarium root rot and wilt diseases of tomato and pepper plants.
Nanomaterials 10 (5): 1001, DOI: 10.3390/nano10051001 Clave: A

116) Nuraini L., Y. Ando, K. Kawai, F. Tatsuzawa, K. Tanaka, M. Ochiai, K. Suzuki, V. Aragonés, J.A. Daròs and T. Nakatsuka (2020).
Anthocyanin regulatory and structural genes associated with violet flower color of Matthiola incana.
Planta 251 (3): 61, DOI:10.1007/s00425-020-03351-z Clave: A

115) Daròs J.A. (2020).
Use of potyvirus vectors to produce carotenoids in plants.
Methods in Molecular Biology (Plant and Food Carotenoids) 2083: 303-312, DOI:10.1007/978-1-4939-9952-1_23 Clave: R

114) Cervera-Seco L., M.C. Marques, A. Sanz-Carbonell, J. Marquez-Molins, A. Carbonell, J.A. Daròs and G. Gomez (2019).
Identification and characterization of a stress-responsive TAS3-derived tasiRNAs in melon.
Plant and Cell Physiology, 60 (11): 2382-2393, DOI: 10.1093/pcp/pcz131 Clave: A

113) Carbonell A., P. Lisón and J.A. Daròs (2019).
Multi-targeting of viral RNAs with synthetic trans-acting small interfering RNAs enhances plant antiviral resistance.
Plant Journal, 100 (4): 720-737, DOI: 10.1111/tpj.14466 Clave: A

112) Pérez-de-Castro A., C. Esteras, A. Alfaro-Fernández, J.A. Daròs, A.J. Monforte, B. Picó and M.L. Gómez-Guillamón (2019).
Fine mapping of wmv1551, a resistance gene to Watermelon mosaic virus in melon.
Molecular Breeding, 39 (7): 93, DOI: 10.1007/s11032-019-0998-z Clave: A

111) Shi X., T. Cordero, S. Garrigues, J.F. Marcos, J.A. Daròs and M. Coca (2019).
Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus.
Plant Biotechnology Journal, 17 (6): 1069-1080, DOI: 10.1111/pbi.13038 Clave: A

110) Pasin F., W. Menzel and J.A. Daròs (2019).
Harnessed viruses in the age of metagenomics and synthetic biology: an update on infectious clone assembly and biotechnologies of plant viruses.
Plant Biotechnology Journal, 17 (6): 1010-1026, DOI: doi: 10.1111/pbi.13084 Clave: R

109) Di Serio F., E.M. Torchetti, J.A. Daròs and B. Navarro (2019).
Reassessment of viroid RNA cytosine methylation status at the single nucleotide level.
Viruses, 11 (4): E357, DOI: 10.3390/v11040357 Clave: A

108) Gobatto D., L.A. de Oliveira, D.A. de Siqueira Franco, N. Velásquez, J.A. Daròs and M. Eiras (2019).
Surveys in the chrysanthemum production areas of Brazil and Colombia reveal that weeds are potential reservoirs of chrysanthemum stunt viroid.
Viruses, 11 (4): E355, DOI: 10.3390/v11040355 Clave: A

107) Aragonés V., A. Pérez-de-Castro, T. Cordero, J. Cebolla-Cornejo, C. López, B. Picó and J.A. Daròs (2019).
A Watermelon mosaic virus clone tagged with the yellow visual maker phytoene synthase facilitates scoring infectivity in melon breeding programs.
European Journal of Plant Pathology, 153: 317-323, DOI: 10.1007/s10658-018-01621-x Clave: A

106) Daròs J.A. (2019).
¿Pueden transformarse los virus de plantas en herramientas útiles para la biotecnología?
Revista Alimentaria, 501: 90-92 Clave: R

105) Carbonell A., C. López and J.A. Daròs (2019).
Fast-forward identification of highly effective artificial small RNAs against different tomato spotted wilt virus isolates.
Molecular Plant-Microbe Interactions, 32 (2): 142-156, DOI: 10.1094/MPMI-05-18-0117-TA Clave: A

104) Carbonell A. and J.A. Daròs (2019).
Design, synthesis, and functional analysis of highly specific artificial small RNAs with antiviral activity in plants.
Methods in Molecular Biology, 2028: 231-246, DOI: 10.1007/978-1-4939-9635-3_13 Clave: R

103) Cordero T., V. Aragonés and J.A. Daròs (2018).
Large-scale production of recombinant RNAs on a circular scaffold using a viroid-derived system in Escherichia coli.
Journal of Visualized Expriments, 141: e58472, DOI: 10.3791/58472 Clave: R

102) Cordero T., A. Rosado, E. Majer, A. Jaramillo, G. Rodrigo and J.A. Daròs (2018).
Boolean computation in plants using post-translational genetic control and a visual output signal.
ACS Synthetic Biology, 7 (10): 2322-2330, DOI: 10.1021/acssynbio.8b00214 Clave: A

101) Cordero T., B. Ortolá and J.A. Daròs (2018).
Mutational analysis of Eggplant latent viroid RNA circularization by the eggplant tRNA ligase in Escherichia coli.
Frontiers in Microbiology 9: 635, DOI: 10.3389/fmicb.2018.00635 Clave: A

100) Daròs J.A., V. Aragonés and T. Cordero (2018).
A viroid-derived system to produce large amounts of recombinant RNA in Escherichia coli.
Scientific Reports 8 (1): 1904, DOI: 10.1038/s41598-018-20314-3 Clave: A

99) Rodrigo G., S. Prakash, S. Shen, E. Majer, J.A. Daròs and A. Jaramillo (2017).
Model-based design of RNA hybridization networks implemented in living cells.
Nucleic Acids Research 45 (16): 9797-9808, DOI: 10.1093/nar/gkx698 Clave: A

98) Daròs J.A. (2017).
Viral suppressors: combating RNA silencing.
Nature Plants 3: 17098, DOI: 10.1038/nplants.2017.98 Clave: R

97) Carbonell A. and J.A. Daròs (2017).
Artificial microRNAs and synthetic trans-acting small interfering RNAs interfere with viroid infection.
Molecular Plant Pathology 18 (5): 746-753, DOI: 10.1111/mpp.12529 Clave: A

96) Abdel-Hafez S.I.I., N.A. Nafady, I.R. Abdel-Rahim, A.M. Shaltout, J.A. Daròs and M.A. Mohamed (2017).
Biosynthesis of silver nanoparticles using the compound curvularin isolated from the endophytic fungus Epicoccum nigrum: characterization and antifungal activity.
Journal of Pharmaceutical and Applied Chemistry 3 (2): 135-146, DOI: 10.18576/jpac/030207

95) López-González S., V. Aragonés, J.A. Daròs, F. Sánchez and F. Ponz (2017).
An infectious cDNA clone of a radish-infecting Turnip mosaic virus strain.
European Journal of Plant Pathology 148: 207-211, DOI: 10.1007/s10658-016-1057-9 Clave: A
94) Cordero T., M.A. Mohamed, J.J. López-Moya and J.A. Daròs (2017).
A recombinant Potato virus Y infectious clone tagged with the Rosea1 visual marker (PVY-Ros1) facilitates the analysis of viral infectivity and allows the production of large amounts of anthocyanins in plants.
Frontiers in Microbiology 8: 611, DOI: 10.3389/fmicb.2017.00611 Clave: A

93) Rodrigo G., J.A. Daròs and S.F. Elena (2017).
Virus-host interactome: putting the accent on how it changes.
Journal of Proteomics 156: 1-4, DOI: 10.1016/j.jprot.2016.12.007 Clave: R

92) Majer E., B. Llorente, M. Rodríguez-Concepción and J.A. Daròs (2017).
Rewiring carotenoid biosynthesis in plants using a viral vector.
Scientific Reports 7: 41645, DOI: 10.1038/srep41645 Clave: A

91) Cordero T., Cerdán L., Carbonell A., Katsarou K., Kalantidis K. and J.A. Daròs (2017).
Dicer-like 4 is involved in restricting the systemic movement of Zucchini yellow mosaic virus in Nicotiana benthamiana.
Molecular Plant-Microbe Interactions 30 (1): 63-71, DOI: 10.1094/MPMI-11-16-0239-R
Clave: A
90) Daròs J.A. (2017).
Eggplant latent viroid.
In Viroids and satellites (editors A. Hadidi, R. Flores, J.W. Randles and P. Palukaitis), Oxford: Academic Press, pp. 339-344. ISBN: 978-0-12-801498-1, Elsevier Inc. Clave: CL

89) Abdel-Hafez S.I.I, N.A. Nafady, I.R. Abdel-RAhim, A.M. Shaltout, J.A. Daròs and M.A. Mohamed (2016).
Assessment of protein silver nanoparticles toxicity against pathogenic Alternaria solani.
3 Biotech 6 (2): 199, DOI: 10.1007/s13205-016-0515-6 Clave: A

88) Daròs J.A. (2016).
Eggplant latent viroid: a friendly experimental system in the family Avsunviroidae.
Molecular Plant Pathology 17 (8):1170-1177, DOI: 10.1111/mpp.12358 Clave: R

87) Willemsen A., M.P. Zwart, N. Tromas, E, Majer, J.A. Daròs and S.F. Elena (2016).
Multiple barriers to the evolution of alternative gene orders in a positive-strand RNA virus.
Genetics 202 (4): 1503-1521, DOI: 10.1534/genetics.115.185017 Clave: A

86) Martínez F., G. Rodrigo, V. Aragonés, M. Ruiz, I. Lodewijk, U. Fernández, S.F. Elena and J.A. Daròs (2016).
Interaction network of tobacco etch potyvirus NIa protein with the host plant during infection.
BMC Genomics 17: 87, DOI: 10.1186/s12864-016-2394-y Clave: A

85) Carbonell A., J.C. Carrington and J.A. Daròs (2016).
Fast-forward generation of effective artificial small RNAs for enhanced antiviral defense in plants.
RNA & Disease 3 (1): e1130. DOI: 10.14800/rd.1130 Clave: R

84) Daròs, J.A. (2016).
Viroids: small noncoding infectious RNAs with the remakable ability of autonomous replication.
En Current Research Topics in Plant Virology (editores Aiming Wang and Xueping Zhou).
Springer International Publishing Switzerland, pp. 295-322. ISBN: 978-3-319-32917-8, ISBN: 978-3-319-32919-2 (eBook), DOI: 10.1007/978-3-319-32919-2_13. Clave: CL

83) Rodrigo G., E. Majer, S. Prakash, J.A. Daròs, A. Jaramillo and J.F. Poyatos (2015).
Exploring the dynamics and mutational landscape of riboregulation with a minimal synthetic circuit in living cells.
Biophysical Journal 109 (5): 1070-1076, DOI: 10.1016/j.bpj.2015.07.021 Clave: A

82) Majer E., J.A. Navarro and J.A. Daròs (2015).
A potyvirus vector efficiently targets recombinant proteins to chloroplasts, mitochondria and nuclei in plant cells when expressed at the amino terminus of the polyprotein.
Biotechnology Journal 10 (11): 1792-1802, DOI: 10.1002/biot.201500042 Clave: A

81) Debreczeni D.E., C. López, J. Aramburu, J.A. Daròs, S. Soler, L. Galipienso, B.W. Falk and L. Rubio (2015).
Complete sequence of three different biotypes of tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain.
Archives of Virology 160 (8): 2117-2123, DOI: 10.1007/s00705-015-2453-8 Clave: A

80) Shen S., G. Rodrigo, S. Prakash, E. Majer, T.E. Landrain, B. Kirov, J.A. Daròs and A. Jaramillo (2015).
Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression.
Nucleics Acids Research 43 (10): 5158-5170, DOI: 10.1093/nar/gkv287 Clave: A

79) Martínez F. and J.A. Daròs (2014).
Tobacco etch virus protein P1 traffics to the nucleolus and associates with the host 60S ribosomal subunits during infection.
Journal of Virology 88 (18): 10725-10737, DOI: 10.1128/JVI.00928-14 Clave: A

78) Majer E., Z. Salvador, M.P. Zwart, A. Willemsen, S.F. Elena and J.A. Daròs (2014).
Relocation of the NIb gene in the tobacco etch potyvirus genome.
Journal of Virology 88 (8): 4586-4590, DOI: 10.1128/JVI.03336-13 Clave: A

77) Gobatto D., A.L.R. Chaves, R. Harakava, J.M. Marque, J.A. Daròs and M. Eiras (2014).
Chrysanthemum stunt viroid in Brazil: survey, identification, biological and molecular characterization and detection methods.
Journal of Plant Pathology 96 (1): 111-119. Clave: A

76) Zwart M.P., A. Willemsen A., J.A. Daròs, S.F. Elena (2014).
Experimental evolution of pseudogenization and gene loss in a plant RNA virus.
Molecular Biology Evolución 31 (1): 121-134, DOI: 10.1093/molbev/mst175 Clave: A

75) Majer E., J.A. Daròs and M.P. Zwart (2013).
Stability and fitness impact of the visually discernible Rosea1 marker in the Tobacco etch virus genome.
Viruses 5 (9): 2153-2168, DOI: 10.3390/v5092153 Clave: A

74) Rodrigo G., T.E. Landrain, E. Majer, J.A. Daròs and A. Jaramillo (2013).
Full design automation of multi-state RNA devices to program gene expression using energy-based optimization.
PLoS Computational Biology 9 (8): e1003172, DOI: 10.1371/journal.pcbi.1003172 Clave: A

73) Martínez F., S.F. Elena and J.A. Daròs (2013).
Fate of artificial microRNA-mediated resistance to plant viruses in mixed infections. Phytopathology 103 (8): 870-876, DOI: 10.1094/PHYTO-09-12-0233-R Clave: A

72) Lafforgue G., F. Martínez, Q.W. Niu, N.H. Chua, J.A. Daròs and S.F. Elena (2013).
Improving the effectiveness of amiRNA-mediated resistance against Turnip mosaic virus by combining two amiRNAs or by targeting highly conserved viral genomic regions.
Journal of Virology 87 (14): 8254-8256, DOI: 10.1128/JVI.00914-13 Clave: A

71) Contreras-Paredes C.A., L. Silva-Rosales, J.A. Daròs, N.D. Alejandri-Ramírez, T.D. Dinkova (2013).
The absence of eukaryotic initiation factor eIF(iso)4E affects the systemic spread of a Tobacco etch virus isolate in Arabidopsis thaliana.
Molecular Plant-Microbe Interactions 26 (4): 461-470, DOI: 10.1094/MPMI-09-12-0225-R
Clave: A
70) Martínez F., G. Lafforgue G, M.J. Morelli, F. González-Candelas, N.H. Chua, J.A. Daròs and S.F. Elena (2012).
Ultra-deep sequencing analysis of population dynamics of virus escape mutants in RNAi-mediated resistant plants.
Molecular Biology Evolution 29 (11): 3297-3307, DOI: 10.1093/molbev/mss135 Clave: A

69) Zwart M., J.A. Daròs and S.F. Elena (2012).
Effects of potyvirus effective population size in inoculated leaves on viral accumulation and the onset of symptoms.
Journal of Virology 86 (18): 9737-9747, DOI: 10.1128/JVI.00909-12 Clave: A

68) Nohales M.A., R. Flores and J.A. Daròs (2012).
Viroid RNA redirects host DNA ligase 1 to act as an RNA ligase.
Proceedings of the National Academy of Sciences of the USA 109 (34): 13805-13810, DOI: 10.1073/pnas.1206187109 Clave: A

67) Nohales M.A., D. Molina-Serrano, R. Flores and J.A. Daròs (2012).
Involvement of the chloroplastic isoform of tRNA ligase in the replication of viroids belonging to the family Avsunviroidae.
Journal of Virology 86 (15): 8269-8276, DOI: 10.1128/JVI.00629-12 Clave: A

66) Sardanyés, J., F. Martínez, J.A. Daròs and S.F. Elena (2012).
Dynamics of alternative modes of RNA replication for positive-sense RNA viruses.
Journal of the Royal Society Interface 9 (69): 768-776, DOI: 10.1098/rsif.2011.0471. Clave: A

65) Molina-Serrano D., J. Marqués J, M.A. Nohales, R. Flores and J.A. Daròs (2012).
A chloroplastic RNA ligase activity analogous to the bacterial and archaeal 2’-5’ RNA ligase.
RNA Biology 9 (3): 326-333, DOI: 10.4161/rna.19218 Clave: A

64) Marqués J., N. Duran-Vila, A. Namsi, J.M. Bové and J.A. Daròs (2012).
Bacteria associated with the rhizosphere of manganese-deficient date palms affected by brittle leaf disease.
Journal of Plant Pathology 94 (1): 157-169. Clave: A

63) Bedoya L.C., F. Martínez, D. Orzáez and J.A. Daròs (2012).
Visual tracking of plant virus infection and movement using a reporter MYB transcription factor that activates anthocyanin biosynthesis.
Plant Physiology 158 (3): 1130-1138, DOI: 10.1104/pp.111.192922 Clave: A

62) De la Iglesia F., F. Martínez, J. Hillung, J.M. Cuevas, P.J. Gerrish, J.A. Daròs and S.F. Elena (2012).
Luria-Delbrück estimation of turnip mosaic virus mutation rate in vivo.
Journal of Virology 86 (6): 3386-3388, DOI: 10.1128/JVI.06909-11 Clave: A

61) Lafforgue, G., F. Martínez, J. Sardanyés, F. de la Iglesia, Q.W. Niu, S.S. Lin, R.V. Solé, N.H. Chua, J.A. Daròs and S.F. Elena (2011).
Tempo and mode of plant RNA virus escape from RNA interference-mediated resistance.
Journal of Virology 85 (19): 9686-9695, DOI: 10.1128/JVI.05326-11 Clave: A

60) Zwart, M., J.A. Daròs and S.F. Elena (2011).
One is enough: in vivo effective population size is dose-dependent for a plant RNA virus.
PLoS Pathogens 7 (7): e1002122, DOI: 10.1371/journal.ppat.1002122 Clave: A

59) Martínez, F., J. Sardanyés, S.F. Elena and J.A. Daròs (2011).
Dynamics of a plant RNA virus intracellular accumulation: stamping machine versus geometric replication.
Genetics 188 (3): 637-646, DOI: 10.1534/genetics.111.129114 Clave: A

58) Marqués, J., N. Duran-Vila and J.A. Daròs (2011).
The Mn-binding proteins of the photosystem II oxygen-evolving complex are decreased in date palms affected by brittle leaf disease.
Plant Physiology and Biochemistry 49 (4): 388-394, DOI: 10.1016/j.plaphy.2011.02.008
Clave: A
57) Eiras, M., M.A. Nohales, E.W. Kitajima, R. Flores and J.A. Daròs (2011).
Ribosomal protein L5 and transcription factor IIIA from Arabidopsis thaliana bind in vitro specifically Potato spindle tuber viroid RNA.
Archives of Virology 156 (3): 529-533, DOI: 10.1007/s00705-010-0867-x Clave: A

56) Flores, R., J.A. Daròs, C. Hernández, F. Di Serio (2011).
Viroids.
Encyclopedia of Life Sciences, DOI: 10.1002/9780470015902.a0000434.pub3. Clave: CL

55) Bedoya, L.C., F. Martínez, L. Rubio and J.A. Daròs (2010).
Simultaneous equimolar expression of multiple proteins in plants from a dissarmed potyvirus vector.
Journal of Biotechnology, 150 (2): 268-275, DOI: 10.1016/j.jbiotec.2010.08.006 Clave: A

54) Eiras, M., S.R. Silva, E.S. Stuchi, R. Flores and J.A. Daròs (2010).
Viroid species associated with the bark-cracking phenotype of ‘Tahiti’ acid lime in the State of São Paulo, Brazill.
Tropical Plant Pathology 35 (5): 303-309. Clave: A

53) Bedoya, L.C. and J.A. Daròs (2010).
Stability of Tobacco etch virus infectious clones in plasmid vectors.
Virus Research 149 (2): 234-240. DOI: 10.1016/j.virusres.2010.02.004 Clave: A

52) Torres-Barceló, C., J.A. Daròs and S.F. Elena (2010).
Compensatory molecular evolution of HC-Pro, an RNA-silencing suppressor from a plant RNA virus.
Molecular Biology and Evolution 27 (3): 543-551, DOI: 10.1093/molbev/msp272
Clave: A
51) Torres-Barceló, C., J.A. Daròs and S.F. Elena (2010).
HC-Pro hypo- and hypersuppressor mutants: differences in viral siRNA accumulation in vivo and siRNA binding activity in vitro.
Archives of Virology 155 (2): 251-254, DOI: 10.1007/s00705-009-0563-x Clave: A

50) Martínez, F., J. Marqués, M.L. Salvador and J.A. Daròs (2009).
Mutational analysis of Eggplant latent viroid RNA processing in Chlamydomonas reinhardtii chloroplast.
Journal of General Virology 90 (12): 3057-3065, DOI: 10.1099/vir.0.013425-0 Clave: A

49) Elena, S.F., G. Gómez and J.A. Daròs (2009).
Evolutionary constraints to viroid evolution.
Viruses, 1 (2): 241-254, DOI: 10.3390/v1020241 Clave: R

48) Flores, R., M.E. Gas, D. Molina-Serrano, M.A. Nohales, A. Carbonell, S. Gago, M. de la Peña and J.A. Daròs (2009).
Viroid replication: Rolling-circles, enzymes and ribozymes.
Viruses, 1 (2): 317-334, DOI: 10.3390/v1020317 Clave: R

47) Serra, P., M. Eiras, S.M. Bani-Hashemian, N. Murcia, E.W. Kitajima, J.A. Daròs, R. Flores and N. Duran-Vila (2008).
Citrus viroid V: occurrence, host range, diagnosis, and identification of new variants.
Phytopathology, 98 (11): 1199-1204, DOI: 10.1094/PHYTO-98-11-1199 Clave: A

46) Torres-Barceló, C., S. Martín, J.A. Daròs and S.F. Elena (2008).
From hypo- to hypersuppression: effect of amino acid substitutions on the RNA-silencing suppressor activity of the Tobacco etch potyvirus HC-Pro.
Genetics, 180 (2): 1039-1049, DOI: 10.1534/genetics.108.091363 Clave: A

45) Gas, M.E., D. Molina-Serrano, C. Hernández, R. Flores and J.A. Daròs (2008).
Monomeric linear RNA of Citrus exocortis viroid resulting from processing in vivo has 5′-phosphomonoester and 3′-hydroxyl termini: implications for the RNase and RNA ligase involved in replication.
Journal of Virology, 82 (20): 10321-10325, DOI: 10.1128/JVI.01229-08 Clave: A

44) Duran-Vila, N., S.F. Elena, J.A. Daròs and R. Flores (2008).
Structure and evolution of viroids.
In Origin and evolution of viruses (2nd edition), E. Domingo, C.R. Parrish and J. Holland (eds.) pp. 43-64. Elsevier. ISBN: 978-0-12-374153. DOI: 10.1016/B978-0-12-374153-0.00002-3 Clave: CL

43) Marqués, J., Z.G.N. Fadda, N. Duran-Vila, R. Flores, J.M. Bové and J.A. Daròs (2008).
A set of novel RNAs transcribed from the chloroplast genome accumulates in date palm leaflets affected by brittle leaf disease.
Phytopathology, 98 (3): 337-344, DOI: 10.1094/PHYTO-98-3-0337 Clave: A

42) Serra, P., C.J. Barbosa, J.A. Daròs, R. Flores and N. Duran-Vila (2008).
Citrus viroid V: molecular characterization and synergistic interactions with other members of the genus Apscaviroid.
Virology, 370 (1): 102-112, DOI: 10.1016/j.virol.2007.07.033 Clave: A

41) Flores, R., M.E. Gas, D. Molina, C. Hernández and J.A. Daròs (2008).
Analysis of viroid replication.
Methods in Molecular Biology, 451: 167-183. Clave: CL

40) Gas, M.E., C. Hernández, R. Flores and J.A. Daròs (2007).
Processing of nuclear viroids in vivo: an interplay between RNA conformations.
PLoS Pathogens, 3 (11): 1813-1826, DOI: 10.1371/journal.ppat.0030182 Clave: A

39) Martin, R., C. Arenas, J.A. Daròs, A. Covarrubias, J.L. Reyes and N.H. Chua (2007).
Characterization of small RNAs derived from Citrus exocortis viroid (CEVd) in infected tomato plants.
Virology, 367 (1): 135-146, DOI: 10.1016/j.virol.2007.05.011 Clave: A

38) Sanjuán, R. and J.A. Daròs (2007).
One-step site-directed mutagenesis of viroid dimeric cDNA.
Journal of Virological Methods, 145 (1): 71-75, DOI: 10.1016/j.jviromet.2007.05.027 Clave: A

37) Eiras, M., E.W. Kitajima, R. Flores and J.A. Daròs (2007).
Existence in vivo of the loop E motif in potato spindle tuber viroid RNA.
Archives of Virology, 152 (7): 1389-1393, DOI: 10.1007/s00705-007-0952-y Clave: A

36) Molina-Serrano, D., L. Suay, M.L. Salvador, R. Flores and J.A. Daròs (2007).
Processing of RNAs of the family Avsunviroidae in Chlamydomonas reinhardtii chloroplasts.
Journal of Virology, 81 (8): 4363-4366, DOI: 10.1128/JVI.02556-06 Clave: A

35) Carrasco, P., J.A. Daròs, P. Agudelo-Romero and S.F. Elena (2007).
A real-time RT-PCR assay for quantifying the fitness of tobacco etch virus in competition experiments.
Journal of Virological Methods, 139 (2): 181-188, DOI: 10.1016/j.jviromet.2006.09.020
Clave: A
34) Namsi, A., J. Marqués, Z. Fadda, M.L. Takrouni, O. Ben Mahamoud, A. Zouba, J.A. Daròs, R. Flores, J.M. Bové and N. Duran-Vila (2006).
Diagnosis of “maladie des feuilles cassantes” or brittle leaf disease of date palms by detection of associated chloroplast encoded double stranded RNAs.
Molecular and Cellular Probes, 20 (6): 366-370, DOI: 10.1016/j.mcp.2006.05.001 Clave: A

33) Codoñer, F.M., J.A. Daròs, R.V. Solé and S.F. Elena (2006).
The fittest versus the flattest: experimental confirmation of the quasispecies effect with subviral pathogens.
PLoS Pathogens, 2 (12): 1187-1193, DOI: 10.1371/journal.ppat.0020136 Clave: A

32) Eiras, M., J.A. Daròs, R. Flores and E.W. Kitajima (2006).
Viróides e virusóides: relíquias do mundo de RNA.
Fitopatologia Brasileira, 31 (3): 229-246, DOI: 10.1590/S0100-41582006000300001 Clave: R

31) Hernández, C., F. Di Serio, S. Ambrós, J.A. Daròs and R. Flores (2006).
An element of the tertiary structure of peach latent mosaic viroid RNA revealed by UV irradiation.
Journal of Virology, 80 (18): 9336-9340, DOI: 10.1128/JVI.00630-06 Clave: A

30) Di Serio, F., J.A. Daròs, A. Ragozzino and R. Flores (2006).
Close structural relationship between two hammerhead viroid-like RNAs associated with cherry chlorotic rusty spot disease.
Archives of Virology, 151 (8): 1539-1549, DOI: 10.1007/s00705-006-0732-0 Clave: A

29) Daròs, J.A., S.F. Elena and R. Flores (2006).
Viroids: an Ariadne’s thread into the RNA labyrinth.
EMBO Reports, 7 (6): 593-598, DOI: 10.1038/sj.embor.7400706 Clave: R

28) Elena, S.F., P. Carrasco, J.A. Daròs and R. Sanjuán (2006).
Mechanisms of genetic robustness in RNA viruses.
EMBO Reports, 7 (2): 168-173, DOI: 10.1038/sj.embor.7400636 Clave: R

27) Flores, R., J.A. Daròs, C. Hernández and F. Di Serio (2005).
Viroids.
En Encyclopedia of Life Sciences, S. Robertson (ed.). Macmillan Reference Limited (Nature Publishing Group), London, U.K. www.els.net Clave: CL

26) Flores, R., C. Hernández, A.E. Martínez de Alba, J.A. Daròs and F. Di Serio (2005).
Viroids and viroid-host interactions.
Annual Reviews in Phytopathology, 43: 117-139, DOI: 10.1146/annurev.phyto.43.040204.140243 Clave: R
25) Daròs, J.A. and R. Flores (2004).
Arabidopsis thaliana has the enzymatic machinery for replicating representative viroid species of the family Pospiviroidae.
Proceedings of the National Academy of Sciences of the USA, 101 (17):6792-6797, DOI: 10.1073/pnas.0401090101 Clave: A

24) Fadda, Z., J.A. Daròs, R. Flores and N. Durán-Vila (2003).
Identification in eggplant of a variant of citrus exocortis viroid (CEVd) with a 96 nucleotide duplication in the right terminal region of the rod-like secondary structure.
Virus Research, 97 (2):145-149, DOI: 10.1016/j.virusres.2003.08.002 Clave: A

23) Triki, M.A., A. Zouba, O. Khoualdia, O. Ben Mahamoud, M.L. Takrouni, M. Garnier, J.M. Bové, M. Montarone, A. Poupet, R. Flores, J.A. Daròs, Z.G.N. Fadda, P. Moreno and N. Durán-Vila (2003).
“Maladie des feuilles cassantes” or brittle leaf disease of date palms in Tunisia: biotic or abiotic disease?
Journal of Plant Pathology, 85 (2): 71-79. Clave: A

22) Fadda, Z., J.A. Daròs, C. Fagoaga, R. Flores and N. Durán-Vila (2003).
Eggplant latent viroid, the candidate type species for a new genus within the family Avsunviroidae (hammerhead viroids).
Journal of Virology, 77 (11): 6528-6532, DOI: 10.1128/JVI.77.11.6528-6532.2003 Clave: A

21) Flores, R., J.A. Daròs and J.A. Navarro (2003).
Replication.
In Viroids, A. Hadidi, R. Flores, J.W. Randles and J.S. Semancik (eds.) pp. 55-60. CSIRO Publishing, Collingwood VIC3066, Australia. ISBN: 0 643 06789 2. Clave: CL

20) Daròs, J.A. and R. Flores (2002).
A chloroplast protein binds a viroid RNA in vivo and facilitates its hammerhead-mediated self-cleavage.
EMBO Journal, 21 (4): 749-759, DOI: 10.1093/emboj/21.4.749 Clave: A

19) Flores, R., C. Hernández, M. de la Peña, A. Vera and J.A. Daròs (2001).
Hammerhead ribozyme structure and function in plant RNA replication.
Methods in Enzymology, 341: 540-552. Clave: R

18) Flores, R., J.A. Daròs, C. Hernández and F. Di Serio (2001).
Viroids.
En Encyclopedia of Life Sciences, S. Robertson (ed.). Macmillan Reference Limited (Nature Publishing Group), London, U.K. www.els.net Clave: CL

17) Vera, A., J.A. Daròs, R. Flores and C. Hernández (2000).
The DNA of a plant retroviroid-like element is fused to different sites in the genome of a plant pararetrovirus and shows multiple forms with sequence deletions.
Journal of Virology, 74 (22): 10390-10400, DOI: 10.1128/JVI.74.22.10390-10400.2000
Clave: A
16) Flores, R., J.A. Daròs and C. Hernández (2000).
Avsunviroidae family: viroids containing hammerhead ribozymes.
Advances in Virus Research, 55: 271-323, DOI: 10.1016/S0065-3527(00)55006-4 Clave: R

15) Daròs, J.A., M. Schaad and J.C. Carrington (1999).
Functional analysis of the interaction between VPg-proteinase (NIa) and RNA polymerase (NIb) of tobacco etch potyvirus, using conditional and suppressor mutants.
Journal of Virology, 73 (10): 8732-8740. Clave: A

14) Navarro, J.A., J.A. Daròs and R. Flores (1999)
Complexes containing both polarity strands of avocado sunblotch viroid: identification in chloroplasts and characterization.
Virology, 253 (1): 77-85, DOI: 10.1006/viro.1998.9497 Clave: A

13) Navarro, B., J.A. Daròs and R. Flores (1998).
Reverse transcription polymerase chain reaction protocols for cloning small circular RNAs.
Journal of Virological Methods, 73 (1): 1-9, DOI: 10.1016/S0166-0934(98)00042-1 Clave: A

12) Haldeman-Cahill, R., J.A. Daròs and J.C. Carrington (1998).
Secondary structures in the capsid protein coding sequence and 3′ nontranslated region involved in amplification of the tobacco etch virus genome.
Journal of Virology, 72 (5): 4072-4079. Clave: A

11) Daròs, J.A. and J.C. Carrington (1997).
RNA binding activity of NIa proteinase of tobacco etch potyvirus.
Virology, 237 (2): 327-336, DOI: 10.1006/viro.1997.8802 Clave: A

10) Di Serio, F., J.A. Daròs, A. Ragozzino and R. Flores (1997).
A 451-nucleotide circular RNA from cherry with hammerhead ribozymes in its strands of both polarities.
Journal of Virology, 71 (9): 6603-6610. Clave: A

9) Daròs, J.A. and R. Flores (1996).
A group I plant intron accumulates as circular RNA forms with extensive 5’ deletions in vivo.
RNA, 2 (9): 928-936. Clave: A

8) Navarro, B., J.A. Daròs and R. Flores (1996).
A general strategy for cloning viroids and other small circular RNAs that uses minimal amounts of template and does not require prior knowledge of its sequence.
Journal of Virological Methods, 56 (1): 59-66, DOI: 10.1016/0166-0934(95)01912-X Clave: A

7) Daròs, J.A. y R. Flores (1995).
Viroides y otros pequeños RNAs circulares e infecciosos de animales y plantas.
En Microbiología y Genética Molecular, Josep Casadesús (ed.) Servicio de Publicaciones de la Universidad de Huelva. ISBN: 84-88751-24-9. Clave: CL

6) Daròs, J.A. and R. Flores (1995).
Characterization of multiple circular RNAs derived from a plant viroid-like RNA by sequence deletions and duplications.
RNA, 1 (7): 734-744. Clave: A

5) Daròs, J.A. and R. Flores (1995).
Identification of a retroviroid-like element from plants.
Proceedings of the National Academy of Sciences of the USA, 92 (15): 6856-6860, DOI: 10.1073/pnas.92.15.6856 Clave: A

4) Daròs, J.A., J.F. Marcos, C. Hernández and R. Flores (1994).
Replication of avocado sunblotch viroid: evidence for a symmetric pathway with two rolling circles and hammerhead ribozyme processing.
Proceedings of the National Academy of Sciences of the USA, 91 (26): 12813-12817, DOI: 10.1073/pnas.91.26.12813 Clave: A

3) Hernández, C., J.A. Daròs, S.F. Elena, A. Moya and R. Flores (1992).
The strands of both polarities of a small circular RNA from carnation self-cleave in vitro through alternative double- and single-hammerhead structures.
Nucleic Acids Research, 20 (23): 6323-6329, DOI: 10.1093/nar/20.23.6323 Clave: A

2) Braco, L., J.A. Daròs and M. de la Guardia (1992).
Enzymatic flow injection analysis in nonaqueous media.
Analytical Chemistry, 64 (2): 129-133, DOI: 10.1021/ac00026a006 Clave: A

1) Rández-Gil, F., J.A. Daròs, A. Salvador and M. de la Guardia (1991).
Direct derivative spectrophotometric determination of nitrazepam and clonazepam in biological fluids.
Journal of Pharmaceutical and Biomedical Analysis, 9 (7): 539-545, DOI: 10.1016/0731-7085(91)80175-9 Clave: A

6) Darós J.A., B. Ortolá and M.T. Cordero.
Method for the production of double-stranded RNA.
Número de solicitud: EP19382997.5, PCT/EP2020/082018
Fecha de solicitud: 13 November 2020.
Entidad titular: CSIC y Universitat Politècnica de València.
Licenciada: Flagship Labs 70, Inc. (Cambridge, MA, USA) desde 26-03-2021 (opción de licencia 11-11-2020).

5) Carbonell A. and J.A. Darós.
Method for modulating the gene silencing degree induced by trans-acting small interfering RNAs.
Número de solicitud: EP19382445.5.
Fecha de solicitud: 31 mayo 2019.
Entidad titular: CSIC y Universitat Politècnica de València.

4) Darós J.A., V. Aragonés and M.T. Cordero.
Recombinant RNA production.
Número de solicitud: EP14382177.5, PCT/EP2015/060912.
Fecha de solicitud: 18 mayo 2015.
Entidad titular: CSIC y Universitat Politècnica de València.
Licenciada: Plant Bioscience Limited (Norwich, Reino Unido) desde 30-07-2013 a 31-12-2018.

3) Darós J.A., L. Bedoya y F. Martínez.
Vector para la coexpresión de varias proteínas heterólogas en cantidades equimolares.
Número de solicitud: P201030342.
País de prioridad: España.
Fecha de solicitud: 9 marzo 2010.
Entidad titular: CSIC y Universitat Politècnica de València.
Referencia: ES 1641.687.
Extensión internacional: PCT/ES11/070150.

2) De la Guardia, M.A., A.E. Morales y J.A. Darós.
Reactor para el empleo de enzimas inmovilizadas en reacción de flujo continuo (FIA) en medios orgánicos no acuosos.
Número de solicitud: 9002666.
País de prioridad: España.
Fecha de anuncio de la concesión: 16-07-1993.
Entidad titular: Universidad de Valencia.
Número de publicación: ES 2 038 563.

1) De la Guardia, M.A., J.V. de Julián, J.A. Darós y S. Garrigues.
Separador circular de fases para diálisis y extracción líquido-líquido en línea.
Número de solicitud: 9002646.
País de prioridad: España.
Fecha de anuncio de la concesión: 16-04-1992.
Entidad titular: Universidad de Valencia.
Número de publicación: ES 2 026 358.

16) Ortolá Navarro, Beltrán (Doctorado en Biotecnología)
Universitat Politècnica de València, 17 de febrero de 2023. Calificación “Sobresaliente Cum laude”, mención doctorado internacional y opta a premio extraordinario
Heterologous expression of circular RNAs in Escherichia coli for analyzing the ligation process of chloroplastic viroids and producing double-stranded RNAs with insecticidal activity
Director: Dr. José Antonio Darós Arnau (IBMCP, CSIC-Universitat Politècnica de València).

15) Martí Botella, María del Carmen (Doctorado en Biotecnología)
Universitat Politècnica de València, 29 de julio de 2022. Calificación “Sobresaliente Cum laude”, mención doctorado internacional
Plant-based production of metabolites and nanoparticles using potyvirus vectors
Director: Dr. José Antonio Darós Arnau (IBMCP, CSIC-Universitat Politècnica de València).

14) Uranga Ruiz de Eguino, Mireia (Doctorado en Biotecnología)
Universitat Politècnica de València, 13 de mayo de 2022. Calificación “Sobresaliente Cum laude”, mención doctorado internacional y opta a premio extraordinario
Engineering viral vectors for CRISPR-Cas mediated genome editing in plants
Director: Dr. José Antonio Darós Arnau (IBMCP, CSIC-Universitat Politècnica de València).

13) Houhou, Fakhreddine (Doctorado en Biotecnología)
Universitat Politècnica de València, 29 de octubre de 2021. Calificación “Sobresaliente Cum laude”
Biotechnological application of potyvirus-based vectors in cucurbits plants
Director: Dr. José Antonio Darós Arnau (IBMCP, CSIC-Universitat Politècnica de València).

12) Cordero Cucart, María Teresa (Doctorado en Biotecnología)
Universitat Politècnica de València, 18 de junio de 2021. Calificación “Sobresaliente Cum laude”
Aplicaciones del factor de transcripción Rosea1 de la ruta de las antocianinas como marcdor visual en virología molecular y biotecnología de plantas.
Director: Dr. José Antonio Darós Arnau (IBMCP, CSIC-Universitat Politècnica de València).

11) Gobatto, Danielle (Doutora em Sanidade, Segurança Alimentar e Ambiental no Agronegócio).
Instituto Biológico, São Paulo, Brasil, 14 de agosto de 2018.
Chrysanthemum stunt viroid: diversidade genética, hospedeiras alternativas, interações viroide-hospedeiro e estudo do impacto na produção do crisãntemo.
Director: Prof. Dr. Marcelo Eiras (Instituto Biológico, São Paulo, Brasil); codirector: Dr. José Antonio Darós Arnau (IBMCP, CSIC-Universitat Politècnica de València).

10) Majer, Eszter (Doctora en Biotecnología)
Universitat Politècnica de València, 14 de julio de 2016. Calificación “Sobresaliente Cum laude”
Metabolic engineering of plants using a disarmed potyvirus vector.
Director: Dr. José Antonio Darós Arnau (IBMCP, CSIC-Universitat Politècnica de València).

9) Martínez García, Fernando
Universitat Politècnica de València, 24 de enero de 2014. Calificación “Sobresaliente Cum laude”
Análisis de la dinámica de replicación de un virus de RNA de plantas y del uso de microRNAs artificiales como estrategia antiviral.
Directores: Dr. José Antonio Darós Arnau y Prof. Santiago Francisco Elena Fito (IBMCP, CSIC-Universitat Politècnica de València).

8) Lafforgue, Guillaume Charles.
Universidad de Valencia, 5 de diciembre de 2013. Calificación “Sobresaliente Cum laude”
Resistance to virus infection mediated by artificial microRNAs: estimating the likelihood of escape mutants.
Directores: Dr. Santiago Francisco Elena Fito (IBMCP, CSIC-Universitat Politècnica de València) y Dr. José Antonio Darós Arnau (IBMCP, CSIC-Universitat Politècnica de València).

7) Bedoya Rojas, Leonor Cecilia.
Universitat Politècnica de València, 28 de noviembre de 2011. Calificación “Sobresaliente Cum laude”
Desarrollo de un vector para la expresión simultánea de múltiples proteínas en plantas basado en el potyvirus del grabado del tabaco.
Director: Dr. José Antonio Darós Arnau (IBMCP, CSIC-Universitat Politècnica de València).

6) Nohales Zafra, María Ángeles.
Universitat Politècnica de València, 26 de septiembre de 2011. Calificación “Sobresaliente Cum laude”
Caracterización de actividades RNA ligasa implicadas en la replicación de los viroides nucleares y cloroplásticos.
Directores: Dr. José Antonio Darós Arnau (IBMCP, CSIC-Universitat Politècnica de València) y Dr. Ricardo Flores Pedauyé (IBMCP, CSIC-Universitat Politècnica de València).

5) Marqués Signes, Jorge.
Universidad de Valencia, 10 de enero de 2011. Calificación “Sobresaliente Cum laude”
Anàlisi molecular de la malastía de les fulles trencadisses de la palmera de dàtils: RNA, proteïnes i bacteries de la rizosfera.
Directores: Dr. José Antonio Darós Arnau (IBMCP, CSIC-Universitat Politècnica de València) y Dra. Nuria Durán-Vila (Instituto Valenciano de Investigaciones Agrarias, Moncada).

4) Torres Barceló, Clara.
Universidad de Valencia, 22 de septiembre de 2009.
Evolució molecular de la proteïna HC-Pro del TEV, supressor del silenciament de l’RNA.
Directores: Dr. Santiago Francisco Elena Fito (IBMCP, CSIC-Universitat Politècnica de València) y Dr. José Antonio Darós Arnau (IBMCP, CSIC-Universitat Politècnica de València).

3) Molina Serrano, Diego.
Universidad de Valencia, 22 de diciembre de 2008. Calificación “Apto Cum laude”
RNA ligasas nucleares y cloroplásticas: posible papel en la replicación de los viroides.
Directores: Dr. José Antonio Darós Arnau (IBMCP, CSIC-Universitat Politècnica de València) y Dr. Ricardo Flores Pedauyé (IBMCP, CSIC-Universitat Politècnica de València).

2) Gas López, María Eugenia.
Universidad de Valencia, 4 de diciembre de 2007.
Replicación de los viroides nucleares: motivos estructurales y enzimas implicados en el procesamiento in vivo de sus intermediarios oligoméricos.
Directores: Dr. Ricardo Flores Pedauyé (IBMCP, CSIC- Universitat Politècnica de València), Cármen Hernández Fort (IBMCP, CSIC-Universitat Politècnica de València) y Dr. José Antonio Darós Arnau (IBMCP, CSIC-Universitat Politècnica de València).

1) Fadda, Ziad Ghaleb Nimer.
Universitat Politècnica de València, 15 de octubre de 2001. Calificación: “Sobresaliente Cum Laude”
Identificación y caracterización de pequeños RNAs en palmera datilera y berenjena.
Directores: Dra. Nuria Durán-Vila (Instituto Valenciano de Investigaciones Agrarias, Moncada), Dr. José Antonio Darós Arnau (IBMCP, CSIC-Universitat Politècnica de València) y Dr. Ricardo Flores Pedauyé (IBMCP, CSIC-Universitat Politècnica de València).