Plant Molecular Virology – Instituto de Biología Celular y Molecular de Plantas (IBMCP)

Research

MAIN RESEARCH LINES

Characterization of essential genes and functions in the infection cycle of viruses belonging to the Ilar and Carmovirus groups, which significantly affect fruit trees and crops of agricultural interest.
Studies on the intra- and intercellular movement of viruses and viroids in their susceptible hosts.
Trafficking of proteins and RNAs through the phloem.
RNA silencing in the pathogenesis process of viruses and viroids.
Development and improvement of new viral diagnostic methods based on the genomic component of viruses.

COLLABORATION WITH INDUSTRY

The advancement in understanding the expression mechanisms of viruses that affect fruit trees, ornamental plants, and horticultural species has enabled their application to solving practical issues caused by certain problematic viral diseases for the corresponding socioeconomic sector. Several research and technology transfer agreements have been established with companies and/or public institutions: Barberet y Blanc S.A., Primaflor S.A., FECOAM, Agromillora Catalana S.A., Plant Health Laboratory-Tenerife, Plant Health Laboratory-Barcelona, Center for Agri-Food Research and Technology of Aragón-Zaragoza, and the National Center for Seeds and Nursery Plants-Zaragoza.

Staff

Staff Researchers

Vicente Pallás Benet

Investigador en Plantilla

Profesor de Investigación CSIC

Contract Staff and Fellows

Irene Máñez Castelló

Investigadora Pre-doctoral

Víctor González Silva

Investigador Pre-doctoral

Publications

2025

Tzanetakis, I.E. et al., (2025). Streamlining Global Germplasm Exchange: Integrating Scientific Rigor and Common Sense to Exclude Phantom Agents from Regulation. Plant Disease https://doi.org/10.1094/PDIS-04-24-0745-FE
Sáiz-Bonilla, M., Li, Y., Montes-Serey, C., Walley, J.W., Dinesh-Kumar, S-P., Pallás, V. & Navarro, J.A. (2025). The proxiome of a plant viral protein with dual targeting to mitochondria and chloroplasts revealed MAPK cascade and splicing components as proviral factors. Plant J. 122, e70161. doi: 10.1111/tpj.70161.
Thompson, J.R., Canto, t., Carr, J.P., Pallás, V. and Šafářová, D. ICTV Report Consortium (2025). ICTV Virus Taxonomy Profile: Bromoviridae. Journal of General Virology 106:002069. DOI 10.1099/jgv.0.002069.
Martínez, C., López, C., Pallás, V., Aparicio, F. and Galipienso, L. (2025). Role of the coat (CP), movement (MP) and 2b proteins of parietaria motte virus (PMoV) as pathogen determinants in Nicotiana benthamiana plants. Eur. J. Plant Pathol., DOI: 10.1007/s10658-025-03002-7
2024
Ontañón, C., Ojinaga, M., Larregla, S., Zabala, J. A., Reva, A., Losa, A., Heribia, R., Bertacca, S., Sanahuja, E., Alfaro-Fernández, A., Font-San Ambrosio, M. I., Corachán, L., Pallás, V., & Sánchez-Navarro, J. A. (2024). Molecular analysis of a Spanish isolate of chili pepper mild mottle virus and evaluation of seed transmission and resistance genes. Eur. J. Plant Pathol. 168(2), 401–418. https://doi.org/10.1007/s10658-023-02765-1.
Leastro, M.O., Pallas, V., Sanchez-Navarro, J.A. (2024). The capsid protein of citrus leprosis virus C shows a nuclear distribution and interacts with the nucleolar fibrillarin protein. Virus Res. 340, 199297.
Leastro, M.O., Kitajima, E.W., Pallas, V., Sanchez-Navarro, J.A. (2024). Rescue of a Cilevirus from infectious cDNA clones. Virus Res. 339, 199264.
Herranz M.C., Navarro J.A., Locascio A., Peña L., Manzanares P., Marcos J.F., Pallás V. (2024). Comparative metabolomic analysis of the phloem sap of nine citrus relatives with different degrees of susceptibility to Huanglongbing disease. Eur. J. Plant Pathol., 170(3), 463–478. DOI: 10.1007/s10658-024-02910-4.
Ojinaga M., Larregla S., Alfaro-Fernández A., Font-San Ambrosio M.I., Pallás V., Sánchez-Navarro J.Á. (2024). The length of the polyprobes and the position of the individual probes in them determine the sensitivity in the detection of viruses affecting pepper crops. Eur. J. Plant Pathol., 169 (2), 421 – 430. DOI: 10.1007/s10658-024-02837-w.
Villar-Álvarez, D.; Leastro, M.O.; Pallas, V.; Sánchez-Navarro, J.Á. (2024). Identification of Host Factors Interacting with Movement Proteins of the 30K Family in Nicotiana tabacum. Int. J. Mol. Sci. 25, 12251. https://doi.org/10.3390/ijms252212251
Villar-Álvarez, D.; Navarro, J.A., Pallas, V.; Sánchez-Navarro, J.Á. (2024). Engineering VIGS Vectors by Modifying Movement Proteins of the 30K Family. Biotechnology Journal, 2024; 19:e202400584. https://doi.org/10.1002/biot.202400584
2023
Sáiz-Bonilla, M., Martín Merchán, A., Pallás, V., Navarro, J.A. (2022). Molecular characterization, targeting and expression analysis of chloroplast and mitochondrion protein import components in Nicotiana benthamiana. Front. Plant Sci. 13, 1040688. DOI: 10.3389/fpls.2022.1040688.
Pallas, V., Serio, F.D., Suzuki, N. (2023). The simplest RNA replicons, viroids: A tribute to Ricardo Flores. Virus Res. 323, 198996. DOI 10.1016/j.virusres.2022.198958.
Marquez-Molins, J., Juarez-Gonzalez, V.T., Gomez, G., Pallas, V., Martinez, G. (2023).Occurrence of RNA post-transcriptional modifications in plant viruses and viroids and their correlation with structural and functional features. Virus Res. 323,198958. DOI 10.1016/j.virusres.2022.198958.
Márquez-Molins, J., Villalba-Bermell, P., Corell-Sierra, J., Pallás, V., Gomez, G. (2023). Integrative time-scale and multi-omics analysis of host responses to viroid infection. Plant Cell Envir. 46 (9): 2909-2927. DOI 10.1111/pce.14647.
Martınez-Perez, M., Aparicio, F., Arribas-Hernandez, L., Tankmar, M.D., Rennie, S., von Bulow, S., Lindorff-Larsen, K., Brodersen, P. and Pallas, V. (2023). Plant YTHDF proteins are direct effectors of antiviral immunity against an N6-methyladenosine-containing RNA virus. EMBO J. 42(18): e113378 DOI 10.15252/embj.2022113378.
De la Torre-Almaraz, R., Vargas, D., Salazar, S.M., Pallas, V., Sanchez-Navarro, J.A. (2023). Agave attenuata and Agave amica, new hosts of Tuberose mild mosaic virus in Mexico. Plant Dis. 107(10), 3324. https://doi.org/10.1094/PDIS-03-23-0549-PDN
Sáiz-Bonilla, M., Martín-Merchán, A., Pallas, V. and Navarro, J.A. (2023). A viral protein targets mitochondria and chloroplasts by subverting general import pathways and specific receptors. J Virol. 97(10: doi 10.1128/jvi.01124-23
2022
Notaguchi, M., Pallas, V., Qiu, J., Wang, X. (2022). Editorial: Systemic RNA Signalling in Plants. Front. Plant Sci. 13, 878728. https://doi.org/10.3389/fpls.2022.878728
Gómez, G., Marquez-Molins, J., Martinez, G., Pallas, V. (2022). Plant epigenome alterations: an emergent player in viroid-host interactions. Virus Res. 318,198844. DOI 10.1016/j.virusres.2022.198844.
Pallas, V., Hernandez, C., Marcos, J.F., Daros, J.A., Ambros, S., Navarro, B., Navarro, J.A., de la Peña, M., Gago-Zachert, S., Gas, M.E., Carbonell, A., Lopez, C., Martínez de Alba, A.E., Di Serio, F., Moreno, P. (2022). In memoriam of Ricardo Flores: The career, achievements, and legacy of an inspirational plant virologist. Virus Res. 312, 198718. https://doi.org/10.1016/j.virusres.2022.198718.
Alvarado-Marchena L, Martínez-Pérez M, Aparicio F, Pallas V and Maumus F (2022) Recent Acquisition of Functional m6A RNA Demethylase Domain in Orchid Ty3/Gypsy Elements. Front. Plant Sci. 13:939843. doi: 10.3389/fpls.2022.939843.
Marquez-Molins, J., Hernandez-Azurdia, A.G., Urrutia-Perez, M., Pallas, V., Gomez, G. (2022). A circular RNA vector for targeted plant gene silencing based on an asymptomatic viroid. Plant J. 112(1), 284-293. https://doi.org/10.1111/tpj.15929.
Alvarado-Marchena, L., Martínez-Pérez, M., Úbeda, J.R., Pallas, V., Aparicio, F. (2022). Impact of the Potential m6A Modification Sites at the 3′UTR of Alfalfa Mosaic Virus RNA3 in the Viral Infection. Viruses 14(8), 1718.
Kuhn, J.H., Adkins, S., Alkhovsky, S.V., (…), Pallas, V.,….Zhang, Y.-Z., Økland, A.L. hasta 181 autores (2022). 2022 taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Arch. Virol. 167 (12): 2857 – 2906. DOI 10.1007/s00705-022-05546-z
Villar-Álvarez, D., Pallás, V., Elena, S.F., Sánchez-Navarro, J.A. (2022). An Evolved 5’-Untranslated Region of Alfalfa Mosaic Virus Allows the RNA Transport of Movement-Defective Variants. J. Virol. 96:e00988-22. https://doi.org/10.1128/jvi.00988-22
Leastro, M., Pallás, V., Sánchez-Navarro, J.Á. (2022). Orchid fleck dichorhavirus movement protein shows RNA silencing suppressor activity. J. Gen. Virol. 103 (11). 11. DOI 10.1099/jgv.0.001805.
Leastro, M.O., Freitas-Astúa, J., Kitajima, E.W., Pallás, V., Sánchez-Navarro, J.A. (2021). Unravelling the involvement of cilevirus p32 protein in the viral transport. Scientific Reports 11 (1), 2943. DOI: 10.1038/s41598-021-82453-4.
Di Serio, F., Owens, R.A., Li, S-H., Matoušek, J., Pallás, V., Randles, J.W., Sano, T., Verhoeven, J.Th.J., Vidalakis, G., Flores, R. and ICTV Report Consortium. (2021). ICTV Virus Taxonomy Profile: Pospiviroidae. Journal of General Virology 102:001543. DOI 10.1099/jgv.0.001543.
Marquez-Molins, J., Gomez, G., Pallas, V. (2021). Hop stunt viroid: A polyphagous pathogenic RNA that has shed light on viroid–host interactions. Molecular Plant Pathology, 22 (2), 153-162. DOI: 10.1111/mpp.13022.
Leastro, M.O., Freitas-Astúa, J., Kitajima, E.W., Pallás, V., Sánchez-Navarro, J.Á. (2021). Membrane association and topology of citrus leprosis virus c2 movement and capsid proteins. Microorganisms, 9 (2), 1-9. DOI: 10.3390/microorganisms9020418.
Vázquez Prol, F., Márquez-Molins, J., Rodrigo, I., López-Gresa, M.P., Bellés, J.M., Gómez, G., Pallás, V. and Lisón, L. (2021). Symptom severity, infection progression and plant responses in solanum plants caused by three pospiviroids vary with the inoculation procedure. Int. J. Mol. Sci. 22, 6189. https://doi.org/10.3390/ijms22126189.
Alvarado-Marchena, L., Marquez-Molins, J., Martinez-Perez, M., Aparicio, F. and Pallas, V. (2021). Mapping of functional subdomains in the atALKBH9B m 6 A- demethylase required for its binding to the viral RNA and to the coat protein of alfalfa mosaic virus. Front. Plant Sci. 12:701683. doi: 10.3389/fpls.2021.701683.
Navarro, J.A., Saiz-Bonilla, M., Sanchez-Navarro, J.A. and Pallas, V. (2021). The mitochondrial and chloroplast dual targeting of a multifunctional plant viral protein modulates chloroplast-to-nucleus communication, RNA silencing suppressor activity, encapsidation, pathogenesis and tissue tropism. Plant J. 108, 197–218 doi: 10.1111/tpj.15435
Marquez- Molins, J., Navarro, J.A., Seco, L.C., Pallas, V. and Gomez, G. (2021). Might exogenous circular RNAs act as protein-coding transcripts in plants?. RNA Biol. 18:sup1, 98-107, DOI: 10.1080/15476286.2021.1962670.
Kuhn, J.H., Adkins, S., Agwanda, B. et al. (2021) Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Arch Virol (2021). https://doi.org/10.1007/s00705-021-05143-6.
Martínez-Pérez M, Gómez-Mena C, Alvarado-Marchena L, Nadi R, Micol JL, Pallas V and Aparicio F (2021) The m6A RNA Demethylase ALKBH9B Plays a Critical Role for Vascular Movement of Alfalfa Mosaic Virus in Arabidopsis. Front. Microbiol. 12:745576. doi: 10.3389/fmicb.2021.745576
Leastro, M.O., Villar-álvarez, D., Freitas-Astúa, J., Kitayima, E.W., Pallás, V., Sánchez-Navarro, J.Á. (2021). Spontaneous mutation in the movement protein of citrus leprosis virus c2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage. Viruses 13(12),2498.
Navarro, J.A., Serra-Soriano, M., Corachán-Valencia, L., Pallás, V. (2020). A conserved motif in three viral movement proteins from different genera is required for host factor recruitment and cell-to-cell movement. Scientific Reports, 10 (1), art. no. 4758. https://doi.org/10.1038/s41598-020-61741-5.
Salavert, F., Navarro, J.A., Owen, C.A., Khechmar, S., Pallás, V., Livieratos, I.C. (2020). Cucurbit chlorotic yellows virus p22 suppressor of RNA silencing binds single-, double-stranded long and short interfering RNA molecules in vitro. Virus Research, 279, art. no. 197887.
Salgado-Ortíz, H., De La Torre-Almaraz, R., Sánchez-Navarro, J.Á., Pallás, V. (2020). Identification and genomic characterization of a novel tobamovirus from prickly pear cactus (2020) Archives of Virology, 165 (3), 781-784.
López-González, S., Navarro, J.A., Pacios, L.F., Sardaru, P., Pallás, V., Sánchez, F. and Ponz, F. (2020). Association between flower stalk elongation, an Arabidopsis developmental trait, and the subcellular location and movement dynamics of the viral non-structural protein P3. Mol. Plant Pathol. 21, 1271–1286.
Leastro MO, Castro DYO, Freitas-Astúa J, Kitajima EW, Pallás V and Sánchez-Navarro JÁ (2020). Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity. Front. Microbiol. 11, 1231. doi: 10.3389/fmicb.2020.01231.
Leastro, M.O., Freitas-Astúa, J., Kitajima, E.W., Pallás, V., Sánchez-Navarro, J.A. (2020). Dichorhaviruses Movement Protein and Nucleoprotein Form a Protein Complex That May Be Required for Virus Spread and Interacts in vivo With Viral Movement-Related Cilevirus Proteins. Front. Microbiol. 11, e571807. DOI: 10.3389/fmicb.2020.571807.
Kuhn, J.H., Pallas, V., et al. (2020) (196 authors). Taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Arch. Virol. 165 (12), 3023-3072.
Martínez-Pérez, M., Navarro, J.A., Pallás, V. and Sánchez-Navarro, J.A (2019)

A sensitive and rapid RNA silencing suppressor activity assay based on alfalfa mosaic virus expression vector.

Virus Research 272, 1977332

Marquez‑Molins, J., Navarro, J.A., Pallas V. and Gomez, G. (2019)

Highly efficient construction of infectious viroid‑derived clones

Plant Methods 15:87
Bujarski, J., Gallitelli, D., García-Arenal, F., Pallás, V., Palukaitis, P., Reddy, M.K., Wang, A (2019)

ICTV Virus Taxonomy Profile: Bromoviridae

Journal of General Virology 100 (8): 1206-1207.
Navarro, J.A., Sanchez-Navarro, J.A., Pallas, V (2019)

Key checkpoints in the movement of plant viruses through the host.

Advances in Virus Research, 104: 1-64.
De La Torre-Almaráz, R., Pallás, V., Sánchez-Navarro, J.A (2019)

Molecular characterization of a new trichovirus from peach in Mexico

Arch. Virol. 164:2617–2620
Sánchez-Navarro, J.Á., Corachán, L., Font, I., Alfaro-Fernández, A., Pallás, V (2019)

Polyvalent detection of twelve viruses and four viroids affecting tomato by using a unique polyprobe

Eur. J. Plant Pathol. 155:361–368.
Di Serio, F., Li, S.-F., Matoušek, J., Owens, R.A., Pallás, V., Randles, J.W., Sano, T., Verhoeven, J.T.J., Vidalakis, G., Flores, R., Lefkowitz, E., Davison, A., Siddell, S., Simmonds, P., Sabanadzovic, S., Smith, D.B., Orton, R. and Zerbini, F.M. (ICTV Report Consortium). (2018)

ICTV virus taxonomy profile: Avsunviroidae

Journal of General Virology 99 (5): 611-612.
Rubio, M., Martínez-Gómez, P., Pallás, V., Dicenta, F., Sánchez-Navarro, J.A. (2018)

Molecular characterization of ilarvirus Prunus necrotic ring spot virus (PNRSV) and Prune dwarf virus (PDV) in Spanish autochthonous almond cultivars.

Acta Horticulturae 1219: 353-356.
Aparicio, F., Aramburu, J., Herranz, M.C., Pallás, V., López, C (2018)

Parietaria mottle virus: A potential threat for tomato crops?.

Acta Horticulturae 1207: 261-268.

Sánchez-Navarro, J.A., Cooper, C.N., and Pallás, V (2018)

Polyvalent detection of members of the genus potyvirus by molecular hybridization using a genus-probe.

Phytopathology 108 (12): 1522-1529

Pallás V, Sánchez-Navarro JA and James D (2018)

Recent Advances on the Multiplex Molecular Detection of Plant Viruses and Viroids

Front. Microbiol. 9:2087
Navarro, J.A. and Pallás, V (2017)

An update on the intracellular trafficking of carmoviruses

Front. Plant Sci. 8, e1801
Martinez-Perez, M. Aparicio, F. Lopez-Gresa, M.P. Belles, J.M., Sanchez-Navarro, J.A. and Pallas, V (2017)

Arabidopsis m(6)A demethylase activity modulates viral infection of a plant virus and the m(6)A abundance in its genomic RNAs

Proc. Natl. Acad. Sci. USA 114: 10755-10760
Serra-Soriano, M., Navarro, J.A. and Pallas, V (2017)

Dissecting the multifunctional role of the N-terminal disordered domain of a plant virus coat protein in RNA packaging, viral movement and interference with antiviral plant defense

Molecular Plant Pathology 18(6): 837-849
Fernández-Crespo, E., Navarro, J.A., Serra-Soriano, M., Finiti, I., García-Agustín, P., Pallás, V. and González-Bosch, C (2017)

Hexanoic Acid Treatment Prevents Systemic MNSV Movement in Cucumis melo Plants by Priming Callose Deposition Correlating SA and OPDA Accumulation

Front. Plant Sci. 8, 1793
Garcia, ML, Dal Bo, E, da Graca, JV, Gago-Zachert, S, Hammond, J, Moreno, P, Natsuaki, T., Pallas, V., Navarro, JA., Reyes, CA., Luna, G.R., Sasaya, T., Tzanetakis, L.E., Vaira, A.M., Verbeek, M (2017)

ICTV Virus Taxonomy Profile: Ophioviridae

J. Gen. Virol. 98: 1161-1162
Sipahioğlu, H.M., Kaya, I., Usta, M., Ünal, M., Özcan, D., Özer, M., Güller, A. and Pallas, V (2017)

Pokeweed (Phytolacca americana) antiviral protein inhibits Zucchini yellow mosaic virus infection in a dose dependent manner in squash plants

Turkish Journal of Agriculture and Forestry 41 (4): 256-262
Rubio, M., Martinez-Gomez, P., Marais, A., Sanchez-Navarro, J.A., Pallas, V., Candresse, T (2017)

Recent advances and prospects in Prunus virology.

Ann. App. Biol. 171: 125-138
Bueso, E., Serrano, R., Pallás, V. and Sánchez-Navarro, J.A (2017)

Seed tolerance to deterioration in arabidopsis is affected by virus infection

Plant Physiology and Biochemistry 116: 1-8
Leastro, Mikhail O.; Pallas, V., Resende, R.O., Sanchez-Navarro, J.A (2017)

The functional analysis of distinct tospovirus movement proteins (NSM) reveals different capabilities in tubule formation, cell-to-cell and systemic virus movement among the tospovirus species

Virus Research 227: 57-68

Leastro, M.O., De Oliveira, A., Pallas, V., Sanchez-Navarro, J.A. Kormelink; R. and Resende, R (2017)

The NSm proteins of phylogenetically related tospoviruses trigger Sw-5b mediated resistance dissociated of their cell-to-cell movement function.

Virus Research 227: 57-68
James D, Cieslinska, M, Pallás V, Flores R, Candresse T, Jelkmann W (2017)

Viruses, viroids, phytoplasmas and genetic disorders of cherry. In Cherries: Genetics, Ecophysiology, Production, Protection and Uses

CABI Press, Oxfordshire. 386-419
Alfaro-Fernández A, Sánchez-Navarro JA, Landeira M, Font MI, Hernández-Llópis D, Pallás V (2016)

Evaluation of PCR and non-radioactive molecular hybridization techniques for the routine diagnosis of Tomato leaf curl New Delhi virus, Tomato yellow leaf curl virus and Tomato yellow leaf curl Sardinia virus

Journal of Plant Pathology 98
Colimba J, Falcon E, Castro ER, Davila-Aldas D, Pallas V, Sanchez-Navarro JA, Gomez G (2016)

First Report of Alfalfa mosaic virus in Red Pepper Plants in Ecuador.

Plant Disease 100: 1026-1026
De La Torre-Almaraz R, Pallas V, Sanchez-Navarro JA (2016)

First Report of Cucumber mosaic virus (CMV) and CARNA-5 in Carnation in Mexico.

Plant Disease 100: 1509-1509
Aparicio F, Pallás V (2016)

The coat protein of Alfalfa mosaic virus interacts and interferes with the transcriptional activity of the bHLH transcription factor ILR3 promoting salicylic acid-dependent defence signalling response

Molecular Plant Pathology 12: 358
Pallas V, Garcia JA (2016)

Viral pathogenesis: still many missing pieces in the puzzle

Current Opinion in Virology 17: V-VII
CastellanoM, Martinez G, Pallas V, Gómez G (2015)

Alterations in host DNA methylation in response to constitutive expression of Hop stunt viroid RNA in Nicotiana benthamiana plants

Plant Pathology 64: 1247-1257
Serra-Soriano M, Navarro JA, Genoves A, Pallas V (2015)

Comparative proteomic analysis of melon phloem exudates in response to viral infection

Journal of Proteomics 124: 11-24
Leastro MO, Pallas V, Resende RO, Sanchez-Navarro JA (2015)

The movement proteins (NSm) of distinct tospoviruses peripherally associate with cellular membranes and interact with homologous and heterologous NSm and nucleocapsid proteins

Virology 478: 39-49

Garcia JA, Pallas V (2015)

Viral factors involved in plant pathogenesis

Current Opinion in Virology 11: 21-30
Serra-Soriano M, Pallas V, Navarro JA (2014)

A model for transport of a viral membrane protein through the early secretory pathway: minimal sequence and endoplasmic reticulum lateral mobility requirements

Plant Journal 77: 863–879
Martinez G, Castellano M, Tortosa M, Pallas V, Gomez G (2014)

A pathogenic non-coding RNA induces changes in dynamic DNA methylation of ribosomal RNA genes in host plants

Nucleic Acids Research 42: 1553–1562
Di Serio F, Flores R, Verhoeven JTJ, Li SF, Pallas V, Randles JW, Sano T, Vidalakis G, Owens RA (2014)

Current status of viroid taxonomy

ARCHIVES OF VIROLOGY 159: 3467-3478
De La Torre-Almaraz R, Sanchez-Navarro J, Pallas V (2014)

DETECTION OF Prunus necrotic ringspot virus FROM PEACH (Prunus persica (L.) IN MEXICO AND MOLECULAR CHARACTERIZATION OF ITS RNA-3 COMPONENT

AGROCIENCIA 48: 583-598
Peiro A, Izquierdo-Garcia AC, Sanchez-Navarro JA, Pallas V Mulet JM, Aparicio F (2014)

Patellins 3 and 6, two members of the Plant Patellin familly, interact with the movement protein of Alfalfa mosaic virus and interfere with the viral movement

Molecular Plant Pathology 15: 881-891
Peiró A, Martínez-Gil L, Tamborero S, Pallás V, Sánchez-Navarro JA, Mingarro I (2014)

The Tobacco Mosaic Virus Movement Protein Associates with but Does Not Integrate into Biological Membranes

Journal of Virology 88: 3016
Martínez C, Coll-Bonfill N, Aramburu J, Pallás V, Aparicio FC, Galipienso L (2014)

Two basic (hydrophilic) regions in the movement protein of Parietaria mottle virus have RNA binding activity and are required for cell-to-cell transport

Virus Research 184: 54-61
Herranz MC, Niehl A, Rosales M, Fiore N, Zamorano A, Granell A, Pallas V (2013)

A remarkable synergistic effect at the transcriptomic level in peach fruits doubly infected by Prunus necrotic ringspot virus and Peach latent mosaic viroid

Virology Journal 10: 1-15
Pallas V, Gomez G (2013)

Phloem RNA-binding proteins as potential components of the long-distance RNA transport system.

Frontiers In Plant Science 4: 130

Fajardo TVM, Peiro A, Pallas V, Sanchez-Navarro JA (2013)

Systemic transport of Alfalfa mosaic virus can be mediated by the movement proteins of several viruses assigned to five genera of the 30K family

Journal of General Virology 94: 677-681
Pallas V, Aparicio F, Herranz MC, Sánchez-Navarro JA, Scott SW (2013)

The Molecular Biology of Ilarviruses

Advances in Virus Research 87: 139-181
Pallas V (2013)

Una breve historia del fenómeno del silenciamiento del RNA. Contribuciones de la Virología a su descubrimiento

Virología 16: 14-21
Gomez G, Pallas V (2013)

Viroids: A light in the darkness of the lncRNA-directed regulatory networks in plants

New Phytologist 198: 10-15
Gomez G, Pallas V (2012)

A pathogenic non coding RNA that replicates and accumulates in chloroplasts traffics to this organelle through a nuclear-dependent step

Plant Signaling & Behaviour 7: 882-884
Pallas V, Aparicio F, Herranz MC, Amari K, Sanchez-Pina A, Myrta A, Sanchez-Navarro JA (2012)

Ilarviruses of Prunus spp.: A Continued Concern for Fruit Trees

Phytopathology 102: 1108-1120
Herranz MC, Pallas V, Aparicio F (2012)

Multifunctional roles for the N-terminal basic motif of Alfalfa mosaic virus coat protein: nucleolar/cytoplasmic shuttling, modulation of RNA-binding activity and virion formation

Molecular Plant Microbe Interactions 25: 1093-1103
Peiro A, Pallas V, Sanchez-Navarro JA (2012)

Simultaneous detection of eight viruses and two viroids affecting stone fruit trees by using a unique polyprobe

European Journal of Plant Pathology 132: 469-475
Gomez G, Pallas V (2012)

Studies on Subcellular Compartmentalization of Plant Pathogenic Noncoding RNAs Give New Insights into the Intracellular RNA-Traffic Mechanisms

Plant Physiology 159: 558-564

Pallas V, Martinez G, Gomez G (2012)

The interaction between plant viroid-induced symptoms and RNA silencing.

Methods in molecular biology (Clifton N.J.) 894: 323-343

Aparicio F, Aparicio-Sanchis R, Gadea J, Sánchez-Navarro JA, Pallás V, Murguia JR (2011)

A plant virus movement protein activates the budding yeast Gcn2p kinase in a Tor1p dependent manner

PLoS ONE 6: e27409
Genovés A, Pallás V, Navarro JA (2011)

Contribution of topology determinants of a viral movement protein on its membrane association, intracellular traffic and viral cell-to-cell movement

Journal of Virology 85: 7797-7809
Martínez G, Forment J, Llave C, Pallás V, Gómez G (2011)

High-throughput sequencing, characterization and detection of new and conserved cucumber miRNAs

PLoS ONE 6: e19523
Pallás V, Garcia JA (2011)

How do plant viruses induce disease? Interactions and interference with host components

Journal of General Virology 92: 2691–2705
Gomez G, Pallas V (2010)

Can the import of mRNA into chloroplasts be mediated by a secondary structure of a small non-coding RNA?

Plant Signaling & Behaviour 5: 1-3
Sanchez-Navarro J, Fajardo T, Zicca S, Pallas, V, Stavolone, L (2010)

Caulimoviridae Tubule-Guided Transport Is Dictated by Movement Protein Properties

Journal of Virology 84: 4109-4112
Garcia-Ibarra A, Martinez-Gomez P, Rubio M, Dicenta F, Soler A, Pallas V, Sanchez-Navarro JA (2010)

First Report of Apricot latent virus and Plum bark necrosis stem pitting-associated virus in Apricot from Spain

Plant Disease 94: 275-275
Martinez G, Donaire L, Llave C, Pallas V, Gomez G (2010)

High-throughput sequencing of Hop stunt viroid-derived small RNAs from cucumber leaves and phloem

Molecular Plant Pathology 11: 347-359
Aparicio F, Pallas V, Sanchez-Navarro J (2010)

Implication of the terminus of the Prunus necrotic ringspot virus movement protein in cell-to-cell transport and in its interaction with the coat protein

Journal of General Virology 91: 1865-1870

Gomez G, Pallas V (2010)

Noncoding RNA Mediated Traffic of Foreign RNA into Chloroplasts Reveals a Novel Signalling Mechanism in Plants

PLoS ONE 5: e12269

Genoves A, Navarro JA, Pallas V (2010)

The Intra- and Intercellular movement of Melon necrotic spot virus (MNSV) depends on an active secretory pathway

Molecular Plant Microbe Interactions 23: 263-272
Genoves A, Navarro JA, Pallas V (2009)

A self-interacting carmovirus movement protein plays a role in binding of viral RNA during the cell-to-cell movement and shows an actin cytoskeleton dependent location in cell periphery

Virology 395: 133-142
De La Torre R, Teliz-Ortiz D, Pallas V, Sanchez-Navarro JA (2009)

First Report of Avocado sunblotch viroid in Avocado from Michoacan, Mexico

Plant Disease 93: 202-202
Aparicio F, Aramburu J, Soler S, Galipienso L, Nuez F, Pallas V, Lopez C (2009)

Immunodiagnosis of Parietaria mottle virus in Tomato Crops Using a Polyclonal Antiserum against its Coat Protein Expressed in a Bacterial System

Journal of Phytopathology 157: 511-513
Gomez G, Martinez G, Pallas V (2009)

Interplay between viroid-induced pathogenesis and RNA silencing pathways

Trends in Plant Sciences 14: 264-269
Martinez-Gil L, Sanchez-navarro JA, Cruz A, Pallas V, Perez-Gi, J, Mingarro I (2009)

Plant Virus Cell-to-Cell Movement Is Not Dependent on the Transmembrane Disposition of Its Movement Protein

Journal of Virology 83: 5535-5543
Hassan M, Gomez G, Pallas V, Myrta A, Rysanek P (2009)

Simultaneous detection and genetic variability of Stone fruit viroids in Czech Republic

European Journal of Plant Pathology 124: 363-368
Alfaro-Fernandez A, Sanchez-Navarro JA, Cebrian MD, Cordoba-Selles MD, Pallas V, Jorda C (2009)

Simultaneous detection and identification of Pepino mosaic virus (PepMV) isolates by multiplex one-step RT-PCR

European Journal of Plant Pathology 125: 143-158
Aparicio F, Soler S, Aramburu J, Galipienso L, Nuez F, Pallas V, Lopez C (2009)

Simultaneous detection of six RNA plant viruses affecting tomato crops using a single digoxigenin-labelled polyprobe

European Journal of Plant Pathology 123: 117-123

Amari K, Burgos L, Pallas V, Sanchez-Pina MA (2009)

Vertical transmission of Prunus necrotic ringspot virus: hitch-hiking from gametes to seedling

Journal of General Virology 90: 1767-1774

Matic S, Minafra A, Sanchez-Navarro JA, Pallas V, Myrta A, Martelli GP (2009)

‘Kwanzan Stunting’ syndrome: Detection and molecular characterization of an Italian isolate of Little cherry virus 1

Virus Research 143: 61-67
Martinez G, Pallas V, Gomez G (2008)

Analysis of symptoms developed in Nicotiana benthamiana plants expressing dimeric forms of Hop stunt viroid

Journal of Plant Pathology 90: 121-124
Gosalvez-bernal B, Genoves A, Navarro Ja, Pallas, V, Sanchez-pina, Ma (2008)

Distribution and pathway for phloem-dependent movement of Melon necrotic spot virus in melon plants

Molecular Plant Pathology 9: 447-461
Mandic B, Al Rwahnih M, Myrta A, Gomez C, Pallás V (2008)

Incidence and Genetic Diversity of Peach Latent Mosaic Viroid and Hop Stunt Viroid in stone fruits in Serbia

European Journal of Plant Pathology 120: 167-176
Belles JM, Lopez-Gresa MP, Fayos J, Pallas V , Rodrigo I, Conejero V (2008)

Induction of cinnamate 4-hydroxylase and phenylpropanoids in virus-infected cucumber and melon plants

Plant Science 174: 524-533
Galipienso L, Herranz MC, Lopez C, Pallas V, Aramburu J (2008)

Sequence analysis within the RNA 3 of seven Spanish tomato isolates of Parietaria mottle virus (PMoV-T) reveals important structural differences with the parietaria isolates (PMoV)

European Journal of Plant Pathology 120: 125-135
Matic,A, Sanchez-Navarro JA, Mandic B, Myrta A, Pallas V (2008)

Tracking three ilarviruses in stone fruit trees throughout the year by ELISA and tissue-printing hybridization

Journal of Plant Pathology 90: 137-141
Gomez G, Martinez G, Pallas V (2008)

Viroid-induced symptoms in Nicotiana benthamiana plants are dependent on RDR6 activity

Plant Physiology 148: 414-423
Gomez G, Pallás V (2007)

A Viroid-derived peptide can be used as a ¨in vivo¨ nucleolar marker

Journal of Virological Methods 144: 169-171

Amari K, Ruiz D, Gómez G, Sánchez-pina MA, Pallás V, Egea J (2007)

An important new apricot disease in Spain is associated with Hop stunt viroid infection

European Journal of Plant Pathology 118: 173-181

arrar S, Choueiri E, Sanchez-Navarro JA, Myrta A, El Zammar S, Savino V, Pallas V (2007)

First report of Apricot latent virus in Lebanon

Journal of Plant Pathology 89: 303-303
Gomez G, Pallás V (2007)

Mature monomeric forms of Hop stunt viroid resist RNA silencing in transgenic plants

Plant Journal 51: 1041-1049
Martinez-Gil L, Sauri A, Vilar M, Pallas VY, Mingarro I (2007)

Membrane insertion and topology of the p7B movement protein of Melon necrotic spot virus (MNSV)

Virology 367: 348-357
Amari K, Diaz-Vivancos P, Pallas V, Sanchez-Pina AY, Hernandez JA (2007)

Oxidative stress induction by Prunus necrotic ringspot virus infection in apricot seeds

Physiologia Plantarum 131: 302-310
Sanchez-Navarro JA, Cañizares MC, Cano E, Pallas V (2007)

Plant tissue distribution and chemical inactivation of six carnation viruses

Crop Protection 26: 1049-1054
Amari K, Burgos L, Pallas V, Sanchez-Pina MA (2007)

Prunus necrotic ringspot virus early invasion and its effects on apricot pollen grain performance

Phytopathology 97: 892-899
Gandia M, Conesa A, Ancillo G, Gadea J, Forment J, Pallas V, Flores R, Duran-vila N, Moreno P, Guerri (2007)

Transcriptional response of Citrus aurantifolia to infection by Citrus tristeza virus

Virology 367: 298-306
Belles Jm, Garro R, Pallas V, Fayos J, Rodrigo I, Conejero V (2006)

Accumulation of gentisic acid as associated with systemic infections but not with the hypersensitive response in plant-pathogen interactions

Planta 223: 500-511
Sanchez-navarro Ja, Herranz Mc, Pallas V (2006)

Cell-to-cell movement of Alfalfa mosaic virus can be mediated by the movement proteins of Ilar-, bromo-, cucumo-, tobamo-, and comoviruses and does not require virion formation

Virology 346: 66-73

Gosalves B, Garcia-castillo S, Pallas V, Sanchez-pina Ma (2006)

Distribution of carnation viruses in the shoot tip: exclusion from the shoot apical meristem

Physiological and Molecular Plant Pathology 69: 43-51

Choueiri E, Myrta A, Herranz Mc, Hobeika C, Digiaro M, Pallas V (2006)

First report of American plum line pattern virus in Lebanon

Journal of Plant Pathology 88: 227-227
El Maghraby I, Sanchez-Navarro JA, Matic S, Myrta A, Pallas V (2006)

First report of two viruses with filamentous particles from stone fruit trees in Egypt

Journal of Plant Pathology 88: S69
Genoves A, Navarro Ja, Pallas V (2006)

Functional analysis of the five melon necrotic spot virus genome-encoded proteins

Journal of General Virology 87: 2371-2380
Gomez G, Pallas V (2006)

Hop stunt viroid is processed and translocated in transgenic Nicotiana benthamiana plants

Molecular Plant Pathology 7: 511-517
Aparicio F, Sanchez-Navarro J, Pallas V (2006)

In vitro and in vivo mapping of the Prunus necrotic ringspot virus coat protein C-terminal dimerization domain by bimolecular fluorescence complementation

Journal of General Virology 87: 1745-1750
Navarro JA, Genoves A, Climent J, SauriA, Martinez-GilL, Mingarro I, Pallas V (2006)

RNA-binding properties and membrane insertion of Melon necrotic spot virus (MNSV) double gene block movement proteins

Virology 356: 57-67
James D, Varga A, Pallas V, Candresse T (2006)

Strategies for simultaneous detection of multiple plant viruses

Canadian Journal of Plant Pathology 28: 16-29
Galipienso L, Herranz MC, Pallas V, Aramburu J (2005)

Detection of a tomato strain of Parietaria mottle virus (PmoV-T) by molecular hybridization and RT-PCR in field samples from north-eastern Spain

Plant Pathology 54: 29-35
Forment J, Gadea J, Huerta L, Abizanda L, Agusti J, Alamar S, Alos E, Andres F, Arribas R, Beltran JP, Berbel A, Blazquez MA, Brumos J, Canas LA, Cercos M, Colmenero-Flores JM, Conesa M, Estables B, Gandia M, Garcia-Martinez JL, Gimeno J, Gisbert A, Gomez G, Gonzalez-Candelas L, Granell A, Guerri J, Lafuente MT, Madueno F, Marcos JF, Marques MC, Martinez F, Martinez-Godoy MA, Miralles S, Moreno P, Navarro L, Pallas V, Perez-Amador MA, Perez-Valle J, Pons C, Rodrigo I, Rodriguez PL, Royo C, Serrano R, Soler G, Tadeo F, Talon M, Terol J, Trenor M, Vaello L, Vicente O, Vidal Ch, Zacarias L, Conejero V (2005)

Development of a Citrus genome-wide EST collection and cDNA microarray as resources for genomic studies

Plant Molecular Biology 57: 375-391

Navarro Ja, Torok Va, Vetten Hj, Pallas V (2005)

Genetic variability in the coat protein genes of geographically distinct isolates of two viruses associated with lettuce big-vein disease: Lettuce big-vein virus and Mirafiori lettuce virus

Archives of Virology 150: 681-694

Navarro JA, Botella F, Marhuenda A, Sastre P, Sanchez-Pina A, Pallas V (2005)

Identification and partial characterization of Lettuce big-vein associated virus and Mirafiori lettuce big-vein virus in common weeds found amongst Spanish lettuce crops and their role in lettuce big-vei disease transmission

European Journal of Plant Pathology 113: 25-34
Gomez G, Torres HY, Pallas V (2005)

Identification of translocatable RNA-binding phloem proteins fron melon, potential components of the long distance RNA transport system

Plant Journal 41: 107-116
Codoñer FM, Cuevas JM, Sanchez-Navarro JA, Pallas V, Elena SF (2005)

Molecular evolution of plant virus family Bromoviridae based on RNA3-encoded proteins

Journal of Molecular Evolution 61: 1-9
Herranz MC, Sanchez-Navarro JA, Sauri A, Mingarro I, Pallas V (2005)

Mutational analysis of the RNA-binding domain of the Prunus necrotic ringspot virus (PNRSV) movement protein reveals its requirement for cell-to-cell movement

Virology 339: 31-41
Sanchez-Navarro JA, Aparicio F, Herranz MC, Minafra A, Myrta A, Pallas V (2005)

Simultaneous detection and identification of eight stone fruit viruses by one step RT-PCR

European Journal of Plant Pathology 111: 77-84
Herranz MC, Sanchez-Navarro JA, Aparicio F, Pallas V (2005)

Simultaneous detection of six stone fruit viruses by non-isotopic molecular hybridization using a unique riboprobe or ‘polyprobe’

Journal of Virological Methods 124: 49-55
Aparicio F, Pallas V (2002)

The molecular variability analysis of the RNA 3 of fifteen isolates of Prunus necrotic ringspot virus sheds light on the minimal requirements for the synthesis of its subgenomic RNA

Virus Genes 25: 75-84
Gomez G, Pallas V (2001)

Identification of an in vitro ribonucleoprotein complex between a viroid RNA and a phloem protein from cucumber plants

Molecular Plant-Microbe Interactions 14: 910-913
Aparicio F, Sanchez-Navarro JA, Olsthoorn RCL, Pallas V, Bol JF (2001)

Recognition of cis-acting sequences in RNA 3 of Prunus necrotic ringspot virus by the replicase of Alfalfa mosaic virus

Journal of General Virology 82: 947-951

Vilar M, Esteve V, Pallas V, Marcos JF, Perez-Paya E (2001)

Structural properties of carnation mottle virus p7 movement protein and its RNA-binding domain

Journal of Biological Chemistry 276: 18122-18129

Amari K, Gomez G, Myrta A, Di Terlizzi B, Pallas V (2001)

The molecular characterization of 16 new sequence variants of Hop stunt viroid reveals the existence of invariable regions and a conserved hammerhead-like structure on the viroid molecule

Journal of General Virology 82: 953-962
Saade M, Aparicio F, Sanchez-Navarro JA, Herranz MC, Myrta A, Di Terlizzi B, Pallas V (2000)

Simultaneous detection of the three ilarviruses affecting stone fruit trees by nonisotopic molecular hybridization and multiplex reverse-transcription polymerase chain reaction

Phytopathology 90: 1330-1336

Projects

PRACTICAS AGRICOLAS RESPETUOSAS CON EL AMBIENTE PARA EL MONITOREO Y OBTENCION DE RESISTENCIAS A ENFERMEDADES VIRALES EN CUCURBITACEAS
Entidad financiadora: Ministerio de Ciencia e innovación-AEI, Plan de Recuperación, Transformación y Resiliencia. TED2021-131949B-I00.
Entidades participantes: Instituto de Biología Molecular y Celular de Plantas. (UPV-CSIC).
Duración: desde: 01- 2023 hasta: 12-2024
Investigador principal: Dr. V. Pallás y Jesus A. Sánchez-Navarro
Número de investigadores participantes: 5
Total Concedido: 200.000 Euros

Threatening emerging diseases for Valencian agriculture: Citrus Huanglongbing (HLB) and tobamoviruses affecting solanaceous and cucurbit crops.
Entidad financiadora: Conselleria D’Eduacçio-GVA (PROMETEO 2023/ CIPROM/2022/31 ).
Entidades participantes: Instituto de Biología Molecular y Celular de Plantas. (UPV-CSIC).
Duración desde: 1/01/2023 hasta: 31/12/2026
Investigador principal: Dr. V. Pallás
Número de investigadores participantes: 7
Total Concedido: 598.000 Euros

‘La modificación n6-metiladenosina (m6a) del RNA y el direccionamiento dual a cloroplastos y mitocondrias como mecanismos reguladores en la biología de los virus RNA de plantas’
Entidad financiadora: Ministerio de Ciencia, Innovación y Universidades – Area Biociencias y Biotecnología (PID2023-149245NB-I00).
Entidades participantes: Instituto de Biología Molecular y Celular de Plantas. (UPV-CSIC).
Duración, desde: 2024 hasta: 2027
Investigador principal: Dr. V. Pallás y F. Aparicio
Número de investigadores participantes: 4
Total Concedido: 262.500 Euros

Identificación y caracterización molecular de nuevos virus y/o aislados que afectanal cultivo de melocotonero en Méjico., 17-01-2010/17-01-2011.

Entidad financiadora: Secretaría de Estado de Cooperación Internacional

IP: Jesús A. Sánchez Navarro

Herramientas genómicas y de transformación genética aplicadas a la mejora de la calidad sanitaria de los frutales de hueso., 01-01-2010/31-12-2011.

Entidad financiadora: Proyecto Colaboración bilateral CSIC-INIA Chile.

IP: Dr. V. Pallás IP parte chilena: Dra. Marlene Rosales

Localización ultraestructural de proteínas de movimiento de dos virus que afectan a cultivos de interés agronómico., 15-12-2009/15-12-2010.

Entidad financiadora: Universidad Politécnica de Valencia

IP: Vicente Pallas Benet.

Interacciones patógeno-huésped en el transporte celular y vascular de virus de interés agronómico. (BIO 2008-03528), 02-01-2009/02-01-2012.

IP: Vicente Pallas Benet

__________________________________________________________________

Ayuda Complementaria al Proyecto ‘Interacciones patogeno-huesped en el transporte celular y vascular de virus de interés agronómico., 01-01-2009/31-12-2011.

Entidad financiadora: Conselleria D’Eduacçio-GVA

IP: Vicente Pallas Benet

Aplicación industrial de la detección simultánea de doce virus que afectan al cultivo del clavel y de gerbera mediante el uso de polisondas (PET2007_0311), 01-09-2008/30-09-2010.

IP: J.A. Sanchez-Navarro

Bases moleculares de la traslocación intercelular y vascular de virus y macromoléculas endógenas en plantas de interés agronómico. (BIO 2005-07331), 31-12-2005/31-12-2008.

IP: Vicente Pallas Benet

Development of diagnostic tools nad host plant resitance to control the rapid spread of lettuce big vein and ring necrosis diseases in leafy vegetables. (Contract QLK5-CT-1999-01471), 30-09-2004/01-10-2004.

IP: Vicente Pallas Benet, (coordinador: H. Vand Heuvel, The Netherlands).

Estudios sobre las rutas y mecanismos de traslocación intercelular y vascular de patógenos virales y subvirales que afectan a frutales de hueso y ornamentales en España. (BIO 2002-04099), 01-10-2002/01-10-2005.

IP: Vicente Pallas Benet

Detección simultánea de los cinco virus y dos viroides que afectan a los frutales de hueso mediante hibridación molecular no radioactiva y multiplex RT-PCR. (PTR1995-0546-OP), 01-10-2001/01-04-2003.

IP: Vicente Pallas Benet

Detection and characterization of Mediterranean of stone fruit viruses viroids and delivery of reliable methods for their routine diagnosis. 01-02-2001/01-01-2006.

Entidad financiadora: Convenio CSIC-CNR

IP: Vicente Pallas Benet

___________________________________________________________________

Puesta a punto de un método de detección basado en la hibridacion molecular para su implementación en el Departamento de Agronomía de la Universidad de Chile 21-05–470/22-05–470.

Entidad financiadora: CSIC

IP: Dr.. V. Pallás (CSIC) y Dr. Jaime Montealegre (U. Chile).

Desarrollo de métodos moleculares para el diagnóstico de las principales virosis del clavel y obtención de plantas transgénicas resistentes al virus del moteado. 15-05–470/16-05–470.

Entidad financiadora: UE- DGICYT

IP: Vicente Pallas Benet

La ‘Viruela’ del albaricoquero en la Región de Murcia., 15-05–470/16-05–470.

Entidad financiadora: Fundación Séneca- Consejería de Agricultura Región de Murcia

IP: Vicente Pallas Benet

Estudios sobre el diagnóstico, variabilidad molecular, transmisibilidad y estrategias de control de la infección de ilarvirus que afectan a frutales de hueso presentes en España. 15-05–470/17-05–470

Entidad financiadora: DGICYT

IP: Vicente Pallas Benet

Desarrollo de nuevas estrategias para el diagnóstico de virosis y para la mejora genética de cultivos frutales y hortícolas de interés regional. 12-05–470/15-05–470.

Entidad financiadora: Consejería de Medio Ambiente, Agricultura y Agua. C.A. Región de Murcia.

IP: Vicente Pallas Benet

Caracterización de genes implicados en el movimiento, replicación y encapsidación en Carmo e Ilarvirus: aplicación a la obtención de plantas transgénicas resistentes a la infección viral. 12-05–470/15-05–470.

Entidad financiadora: DGICYT

IP: Vicente Pallas Benet

Métodos de diagnóstico no radioactivos basados en la hibridación molecular y su aplicación a las virosis del clavel. 11-05–470/12-05–470.

Entidad financiadora: CICYT.

IP: Vicente Pallas Benet

Estudio de las enfermedades de degeneración de árboles frutales inducidas por los viroides: Diagnostico, Caracterización, Epidemiologia y Eliminación. 10-05–470/12-05–470.

Entidad financiadora: CEE.

IP: Vicente Pallas Benet

Estudios a nivel celular y molecular de los mecanismos y efectos de la infección viral en la célula vegetal. 08-05–470/11-05–470.

Entidad financiadora: DGICYT.

IP: Vicente Pallas Benet

Convocatoria de Ayudas para contratos de Personal Técnico de Apoyo. ( PTA2013-8392-I) Mª Angeles Pinto Sánchez.

Entidad financiadora: MINISTERIO DE ECONOMIA Y COMPETITIVIDAD

IP: Vicente Pallas Benet

Patent

Título:

PROCEDIMIENTO PARA LA DETECCIÓN SIMULTÁNEA DE VIRUS DE PLANTAS MEDIANTE EL USO DE POLISONDAS

Referencia ID:

24-05–470

Autores:

PALLAS, V., APARICIO, F., SANCHEZ-NAVARRO, JA. Y HERRANZ, MC.

Título:

Simultaneous detection and identification of specific pepino mosaic virus (PepMV) isolates in a plant sample comprises extraction of total RNA, RNA analysis, identification of amplification products, and digestion with restriction enzymes

Referencia ID:

25-05–470

Autores:

ALFARO FERNANDEZ A, CEBRIAN MICO M D C, CORDOBA SELLES C, GARCIA RANDEZ A, JORDA GUTIERREZ M C, PALLAS BENET V, SANCHEZ NAVARRO J A, CEBRIAN MICO M C

Thesis

Título: Involvement of the host RNA N6-adenosine methylation (m6A) pathway in the infection cycle of Alfalfa mosaic virus.

Doctorando: Mireya Martinez Perez. Universidad: Politécnica de Valencia. Facultad / Escuela: Agrónomos

Año: 2020. Lectura 23 octubre 2020. Calificación: Sobresaliente cum laude. Mención Europea.

Co-director: F. Aparicio. Publicaciones SCI derivadas de la Tesis: 3.

Título: Estudio de las alteraciones en el patrón de metilación del DNA del huésped inducidas por un viroide nuclear.

Doctorando: Mayte Castellano Perez

Universidad: Politécnica de Valencia. Facultad / Escuela: Agrónomos

Año: 2017. Lectura 13 Enero 2017

Calificación: Sobresaliente cum laude. Publicaciones SCI derivadas de la Tesis: 4.

Co-director: Gustavo Gomez

Título: Relación estructura-función de las proteínas virales implicadas en el movimiento de los carmovirus y su interacción con factores celulares.

Doctorando: Marta Serra Soriano

Universidad: Politécnica de Valencia. Facultad / Escuela: Agrónomos

Año: 2016. Lectura 18 Enero 2016

Calificación: Sobresaliente cum laude. Publicaciones SCI derivadas de la Tesis: 4.

Co-director: Jose A. Navarro Bohigues

Título: Proteínas de movimiento de la familia 30K: interacción con membranas biológicas y factores proteicos y su implicación en el transporte viral.

Doctorando: Ana Peiro Morell

Universidad: Politécnica de Valencia. Facultad / Escuela: Agrónomos

Año: 2014. Lectura 4 Diciembre 2014

Calificación: Sobresaliente cum laude. Publicaciones SCI derivadas de la Tesis: 3.

Co-director: Jesus A. Sanchez Navarro

Título: Relación entre el silenciamiento de RNA y la patogénesis inducida por un viroide con replicación nuclear.

Doctorando: Germán E. Martinez Arias

Universidad: Politécnica de Valencia. Facultad / Escuela: Agrónomos

Año: 2011. Lectura mayo 2011

Calificación: Sobresaliente cum laude. Publicaciones SCI derivadas de la Tesis: 5.

Co-director: Gustavo Gómez

Análisis funcional y localización subcelular de las proteínas implicadas en el movimiento intra e intercelular del virus de las manchas necróticas del melon (MNSV)

Año: 2008

Nombre: Genovés Martínez, Ainhoa

Universidad: Universidad Politécnica de Valencia, Escuela de Ingenieros Agrónomos

Dirigida por: Pallás, Vicente; Navarro Bohigues, Jose A.

Tipo: Tesis Doctorales

Mecanismos de transmisión por gametos y semilla en albaricoquero (Prunus armeniaca L.) de un aislado español del virus de los anillos necróticos de los Prunus (PNRSV) y su caracterizaciónm molecular

Año: 2005

Nombre: Amari, Khalid

Universidad: Universidad Politécnica de Cartagena

Dirigida por: Pallas, Vicente; Sanchez-Pina, Amelia.

Tipo: Tesis Doctorales

Estudios sobre el movimiento intercelular del virus de los anillos necróticos de los Prunus y desarrrollo de nuevos métodos de diagnóstico para la detección simultánea de los principales virus que afectan a los frutales de hueso

Año: 2005

Nombre: Herranz Gordo, M Carmen

Universidad: Universidad de Valencia, Facultad de Ciencias Biológicas

Dirigida por: Pallas, Vicente

Tipo: Tesis Doctorales

Distribución de los principales virus de clavel y del virus de las manchas necróticas del mellón (MNSV) en tejidos meristemáticos

Año: 2004

Nombre: Gosálvez Bernal, Blanca

Universidad: Universidad de Murcia, Facultad de Ciencias Biológicas

Dirigida por: Pallas, Vicente; Sanchez-Pina, Amelia.

Tipo: Tesis Doctorales

Estudios sobre la transmisión, variabilidad molecular e interacción proteína de cubierta-RNAs genómicos en el Virus de los anillos necróticos de los Prunus (PNRSV)

Año: 2001

Nombre: Aparicio Herrero, Frederic

Universidad: Universidad de Murcia, Facultad de Ciencias Biológicas

Dirigida por: Pallás, Vicente

Tipo: Tesis Doctorales

Estudios sobre el diagnóstico y la variabilidad molecular del Virus del moteado del clavel

Año: 2001

Nombre: Cañizares Nolasco, Maria Carmen

Universidad: Universidad de Murcia, Facultad de Ciencias Biológicas

Dirigida por: Pallás, Vicente

Tipo: Tesis Doctorales

Análisis molecular y celular de las rutas de invasión del Virus del moteado del clavel en su huésped natural

Año: 2001

Nombre: García Castillo, Silvia

Universidad: Universidad de Murcia, Facultad de Ciencias Biológicas,

Dirigida por: Pallás, Vicente

Tipo: Tesis Doctorales

Caracterización molecular del virus de los anillos necróticos de los Prunus (PNRSV). Desarrollo de métodos moleculares para el diagnóstico del PNRSV y su aplicación a las principales virosis del clavel (Dianthus caryophyllus L.).

Año: 1998

Nombre: Sánchez-Navarro, Jesús A

Universidad: Universidad de Murcia, Facultad de Ciencias Biológicas

Dirigida por: Pallás, Vicente

Tipo: Tesis Doctorales

TRABAJOS FIN DE CARRERA

Subcellular localization of the movement proteins of Carnation mottle virus (CarMV)

Año: 2008

Nombre: Lobbens, Inki

Universidad: Universidad Politénica de Valencia y University College “Karel de Grote-Hogeschool” en Antwerp (Bélgica)

Dirigida por: Pallás, Vicente; Navarro Bohigues, Jose Antonio; Jaeken, Laurent.