QUANTIFICATION OF PLANT HORMONES

The Plant Hormone Quantification Service quantifies hormones using UHPLC-mass spectrometry equipment (Q-Exactive, ThermoFisher Scientific) installed at the IBMCP, capable of quantifying plant hormones in samples of only 50 mg of fresh matter and concentrations below 0.1 ng/g. To date, this service has carried out analyzes of gibberellins (GAs), auxins (IAA), jasmonic acid (JA), salicylic acid (SA), abscisic acid (ABA) and cytokinins (CKs) for a large number of research groups. of the Spanish scientific community and has established collaborations with groups from other countries.

Hormones analyzed in the service:

• Acid hormones (ionization in negative mode):
  • Active gibberellins: GA1 and GA4.
  • Other acid hormones. Includes indole-3-acetic acid (IAA), abscisic acid (ABA), jasmonic acid (JA), and salicylic acid (SA).
  • Other gibberellins (including precursors and inactive GAs) up to a total of 14 GAs from the GA1 (GA1, GA20, GA19, GA44, GA53, GA29 and GA8) and GA4 (GA4, GA9, GA24, GA15) biosynthetic pathways , GA12, GA51 and GA34).

• Basic hormones (positive mode ionization):

• • Cytokinins (CKs). Includes: dihydrozeatin (DHZ), isopentenyladenine (iP), and t-zeatin (tZ).

Conditions of Service:

To request hormone tests, the petitioner must request a quote from the IBMCP Hormone Quantification Service by email (hormonas@ibmcp.upv.es) specifying the type of material, number of samples and hormones to be analysed. inform you of the next steps to follow. Our team only performs hormone quantification, as a service, for Spanish public institutions. In the case of institutions outside this area, we only consider analyzing hormones within the framework of a scientific collaboration.

IBMCP applicants must contact the 2.06 laboratory and fill in the Application Sheet and the Bulletin of Material to Analyze, that They must be presented together with the samples.