Plant hormone quantification

The Hormonomic Service at the Institute of Plant Molecular and Cellular Biology (IBMCP) provides specialized analytical services for the identification and accurate quantification of plant hormones and related compounds in plant tissues, fertilizers, biofertilizers, and other plant-derived samples.

The service is equipped with a UHPLC–mass spectrometry system (Q-Exactive, Thermo Fisher Scientific) capable of quantifying plant hormones in a wide variety of plant samples and derivatives, using either fresh or lyophilized material.

With 15 years of experience, the Service combines advanced analytical technology with extensive scientific and technical expertise in plant hormone analysis. Our team provides tailored analytical solutions to support research and development projects in both academic and industrial settings.

The Service offers the analysis of more than 35 hormone forms and related compounds, providing a comprehensive profile of plant hormone composition and metabolism. These include gibberellins, auxins, jasmonates, salicylates, abscisic acid, and cytokinins, as well as their precursors and catabolic or conjugated forms.

Beyond analytical measurements, the Service provides scientific and technical consulting to help define appropriate analytical strategies according to the objectives, sample type, and characteristics of each project.

Our experience includes the analysis of plant material and the characterization of biofertilizers, as well as studies related to plant growth, development, stress responses, and plant hormone metabolism.

· Samples Preparation and Shipment

· Hormone List and Pricing

Requests from internal users (IBMCP):

1. Initial contact via email: hormonas@ibmcp.upv.es Indicate the experimental design, type of material, number of replicates/samples and hormones requested.

2. Formal request Process through GestCompras.

3. ID assignment The Service assigns an ID to each sample, which must be indicated on the tube cap.

4. Documentation Download, fill out and send via email (hormonas@ibmcp.upv.es).

· IBMCP request form

· Sample registration form

5. Sample delivery → Lab 2.06 (preferably in the morning)

Requests from external users (UPV, CSIC, Universities, AGE, OPIs and Companies):

1. Initial contact via email: hormonas@ibmcp.upv.es After reviewing the documents attached on this website (Rules, requests and pricing), indicate the experimental design, type of material, number of replicates/samples and hormones requested. If scientific advice is needed for the experimental design or the choice of hormones to be analyzed, please contact us by phone or email.

2. Documentation. Download, fill out and send via email the sample Sample registration form with the required information.

3. Administrative management. The user must provide the Service with the following information:

· Name of the Institution and PI responsible for the project

· Email of the responsible PI

· Full tax/billing address of the paying entity

· Indicate invoicing method:

o Invoice (indicate DIR3) o Inter-center transfer (CSIC) o Budget modification (UPV)

The user commits to accepting and paying for the analysis through the signed and stamped Order Form, a document that constitutes the formal request for the analysis and is also essential to begin sample processing. After results are delivered, a Delivery Note is sent, which the user must return signed and stamped so that the invoice can be generated, according to the corresponding administrative deadlines.

The hormonomics service has advanced equipment for the analysis and quantification of plant hormones:

 

1. Instrumentation for hormone extraction and purification:

a) Refrigerated centrifuge (Eppendorf)

b) Vxr Basic shaker motor (VIBRAX)

c) SPE filtration equipment (VacMaster)

d) SAVANT SPD1010 rotary evaporator (ThermoScientific)

 

2. Instrumentation for hormone analysis:

a) VANQUISH automatic sampler (ThermoFisher)

b) VANQUISH chromatographic column oven (ThermoFisher)

c) Q-Exactive mass spectrometer, with high-resolution, high-mass-accuracy Orbitrap analyzer (ThermoFisher)

d) VANQUISH UHPLC high-performance liquid chromatograph (ThermoFisher)

 

Method characteristics: Limit of quantification (LOQ): 0.01 ppb