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Tissue culture and plant breeding

Tissue culture and plant breeding

Research line: Biotechnology and Plant Breeding of Cultivated Species

Tissue culture and plant breeding

Research line: Biotechnology and Plant Breeding of Cultivated Species

Tissue culture and plant breeding
  • Research
  • Staff
  • Publications
  • Projects
  • Patent

Research

The main objective is to develop and apply various biotechnological approaches for the genetic improvement of horticultural species (melon, watermelon, cucumber, and tomato) and ornamental plants. The techniques we use for this purpose include:

  • Genetic transformation of horticultural crops to enhance tolerance to salinity and water stress, resistance to diseases, genetic modification of developmental traits, and quality improvement.
  • Genetic transformation of ornamental species to delay senescence and genetically modify developmental traits.
  • Functional genomics through insertional mutagenesis, trapping, PTGS, etc.
  • Protoplast fusion to obtain new allopolyploids and asymmetric hybrids.
  • Production of haploids and double-haploids in horticultural species.
  • Somaclonal selection of ornamental plants.
  • Micropropagation of carnivorous and ornamental plant species.

Horticultural species: Tomato (Solanum lycopersicum), Melon (Cucumis melo L.), Watermelon (Citrullus lanatus Thunb. [Matsum. & Nakai]), Cucumber (Cucumis sativus L.).

Related wild species of the genera: Solanum sp., Cucumis sp., and Citrullus sp.

Ornamental plants: Ficus benjamina, F. lyrata, F. elastica, Codiaeum variegatum, Kalanchoe blossfeldiana, Begonia sp., Philodendron sp., Spathiphyllum sp., Syngonium sp., Columnea sp., Schlumbergera sp., Hatiora sp., Pelargonium × hortorum, Pelargonium peltatum, and Pelargonium domesticum.

Carnivorous plants: Dionaea muscipula, Drosera aliciae, D. binata, D. capensis typica, D. capensis alba, D. nidiformis, D. spatulata, D. regia, D. nidiformis, Drosophyllum lusitanicum, Byblis liniflora, Sarracenia × moorei, Darlingtonia californica, and others.

Staff

Publications

  • Pérez-Martín, F; Yuste-Lisbona, F; Pineda, B; García-Sogo, B; del Olmo, I ; Alché, JD; Egea, I; Flores, F; Pineiro, M; Jarillo, J; Angosto, T; Capel, J; Moreno, V; Lozano, R. (2018)
    Developmental role of the tomato mediator complex subunit MED18 in pollen ontogeny.

    Plant Journal 96: 300 – 315

  • Egea I, Pineda B, Ortíz-Atienza A, Plasencia F, Drevensek S, García-Sogo B, Yuste-Lisbona F, Barrero J, Atarés A, Flores F, Barneche F, Angosto T, Capel C, Salinas J, Vriezen W, Esch E, Bowler C, Bolarín MC, Moreno V, Lozano R (2018)
    Tomato SlCBL10 ensures plant growth under salt stress by regulating Na+ and Ca2+ homeostasis

    Plant Physiology, 176: 1676 – 1693

  • Pérez-Martín F, Yuste-Lisbona F, Pineda B, Angarita-Díaz M, García-Sogo B, Antón T, Sánchez S, Giménez-Caminero E, Atarés A, Fernández-Lozano A, Ortíz-Atienza A, García-Alcázar M, Castañeda L, Fonseca R, Capel C, Goergen G, Sánchez J, Quispe J, Capel J, Angosto T, Moreno V, Lozano R (2017)

    A collection of enhancer trap insertional mutants for functional genomics in tomato

    Plant Biotechnology Journal 15, 1439–1452

  • García-Alcázar M, Giménez E, Pineda B, Capel C, García-Sogo B, Sánchez S, Yuste-Lisbona FJ, Angosto T, Capel J, Moreno V, Lozano R (2017)
    Albino T-DNA tomato mutant reveals a key function of 1-deoxy-D-xylulose-5-phosphate synthase (DXS1) in plant development and survival

    Scientific Reports 7: 45333

  • Jaime-Pérez N, Pineda B, Garcia-Sogo B, Atares A, Athman A, Byrt CS, Olias R, Asins MJ, Gilliham M, Moreno V, Belver A. (2017)
    The Na+ transporter encoded by the HKT1;2 gene modulates Na+/K+ homeostasis in tomato shoots under salinity

    Plant, Cell & Environment 40: 658-671

  • Rojas-Gracia P, Roque E, Medina M, Rochina M, Hamza R, Angarita-Díaz MP; Moreno V, Pérez-Martín F; Lozano R, Cañas L, Beltrán JP, Gómez-Mena C (2017)
    The parthenocarpic hydra mutant reveals a new function for a SPOROCYTELESS-like gene in the control of fruit set in tomato

    New Phytologist 214 (3) 1198-1212

  • Yuste-Lisbona FJ, Quinet M, Fernández-Lozano A, Pineda B, Moreno V, Angosto T, Lozano R (2016)
    Characterization of vegetative inflorescence (mc-vin) mutant provides new insight into the role of MACROCALYX in regulating inflorescence development of tomato

    Scientific Reports 6: 18796

  • Yuste-Lisbona FJ, Quinet M, Fernández-Lozano A, Pineda B, Moreno V, Angosto T & Lozano R (2016)
    Characterization of vegetative inflorescence (mc-vin) mutant provides new insight into the role of MACROCALYX in regulating inflorescence development of tomato

    Scientific Reports 6: 18796

  • Campos JF, Cara B, Pérez-Martín F, Pineda B, Egea I, Flores FB, Fernandez-Garcia N, Capel J, Moreno V, Angosto T, Lozano R, Bolarin MC (2016)
    The tomato mutant ars1 (altered response to salt stress 1) identifies an R1-type MYB transcription factor involved in stomatal closure under salt acclimation

    Plant Biotechnology Journal 14: 1345-1356

  • Campos J, Cara B, Pérez-Martín F, Pineda B, Egea I, Flores F, Fernández Garcia N, Capel J, Moreno V, Angosto T, Lozano R, Bolarín MC (2016)
The tomato mutant ars1 (altered response to salt stress 1) identifies an R1-type MYB transcription factor involved in stomatal closure under salt acclimation

Plant Biotechnology Journal 14 (6): 1345-1356

  • Gimenez E, Castañeda L, Pineda B, Pan IL, Moreno V, Angosto T, Lozano R (2016)
    TOMATO AGAMOUS1 and ARLEQUIN/TOMATO AGAMOUS-LIKE1 MADS-box genes have redundant and divergent functions required for tomato reproductive development

    Plant Molecular Biology

  • Gimenez E, Castañeda L, Pineda B, Pan, IL, Moreno V, Angosto T, Lozano R (2016)
    TOMATO AGAMOUS1 and ARLEQUIN/TOMATO AGAMOUS-LIKE1 MADS-box genes have redundant and divergent functions required for tomato reproductive development.

    Plant Molecular Biology. 91: 513–531

  • Fernandez-Lozano A, Yuste-Lisbona FJ, Perez-Martin F, Pineda B, Moreno V, Lozano R, Angosto T (2015)
    Mutation at the tomato EXCESSIVE NUMBER OF FLORAL ORGANS (ENO) locus impairs floral meristem development, thus promoting an increased number of floral organs and fruit size

    Plant Science. 232: 41-48

  • Fernandez-Lozano A, Yuste-Lisbona FJ, Perez-Martin F, Pineda B, Moreno V, Lozano R, Angosto T (2015)
    Mutation at the tomato EXCESSIVE NUMBER of FLORAL ORGANS (ENO) locus impairs floral meristem development,thus promoting an increased number of floral organs and fruit size

    Plant Science 232: 41-48

  • Garcia-Abellan J, Fernandez-Garcia N, Lopez-Berenguer C, Egea I, Flores F, Angosto T, Capel J, Lozano R, Pineda B, Moreno V, Olmos E, Bolarin, MC (2015)
    The tomato res mutant which accumulates JA in roots in non-stressed conditions restores cell structure alterations under salinity

    Physiologia Plantarum 155: 296–314

  • Giménez E, Dominguez E, Pineda B, Heredia A, Moreno V, Lozano R, Angosto T (2015)
    Transcriptional Activity of the MADS Box ARLEQUIN/TOMATO AGAMOUS-LIKE Gene Is Required for Cuticle Development of Tomato Fruit

    Plant Physiology 168: 1036-1048

  • Giménez E, Domínguez E, Pineda B, Heredia A, Moreno V, Lozano R, Angosto T (2015)
    Transcriptional activity of the MADS Box ARLEQUIN/TOMATO AGAMOUS-LIKE1 gene is required for cuticle development of tomato fruit

    Plant Physiology 168 (3): 1036-1048

  • García-Abellan JO, Egea I, Pineda B, Sanchez-Bel P , Belver A, Garcia-Sogo B, Flores FB, Atares A, Moreno V, Bolarin MC (2014)
    Heterologous expression of the yeast HAL5 gene in tomato enhances salt tolerance by reducing shoot Na+accumulation in the long term

    Physiologia Plantarum

  • Molina MC, Lopez CG, Staltari S, Chorzempa SE, Moreno V (2013)
    Cryptic homoeology analysis in species and hybrids of genus Zea

    Biologia Plantarum 57: 449-456

  • Juarez P, Presa S, Espi J, Pineda B, Anton MT, Moreno V, Buesa J, Granell A, Orzaez D (2012)
    Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes

    Plant Biotechnology Journal 10: 341-352

  • Munoz-Mayor A, Pineda B, Garcia-Abellan JO, Anton T, Garcia-Sogo B, Sanchez-Bel P, Flores FB, Atares A, Angosto T, Pintor-Toro JA, Moreno V, Bolarin MC (2012)
    Overexpression. of dehydrin tas14 gene improves the osmotic stress imposed by drought and salinity in tomato

    Journal of Plant Physiology 169: 459-468

  • Pineda B, Giménez E, García-Sogo B, Antón T, Atarés A, Capel J, Lozano R, Angosto T, Moreno V (2010)
    Genetic and Physiological Characterization of the Arlequin Insertional Mutant Reveals a Key Regulator of Reproductive Development in Tomato

    Plant Cell Physiology 51: 435-447

  • Estornell LH, Orzaez D, Lopez-Pena L, Pineda B, Anton MT, Moreno V, Granell A (2009)
    A multisite gateway-based toolkit for targeted gene expression and hairpin RNA silencing in tomato fruits

    Plant Biotechnology Journal 7: 298-309

  • Muñoz-Mayor,A, Pineda B, Garcia-Abellan J, Garcia-Sogo B, Moyano E, Atares A, Vicente-Agull F, Serrano R, Moreno V, Bolarin MC (2008)
    The HAL1 function on Na+ homeostasis is maintained over time in salt-treated transgenic tomato plants, but the high reduction of Na+ in leaf is not associated with salt tolerance

    Physiologia Plantarum 133: 288-297

  • Cuartero J, Bolarín MC, Asíns MJ, Moreno V (2006)
    Increasing salt tolerance in the tomato

    Journal of Experimental Botany 57: 1045-1058

  • Ellul P, Angosto T, García-Sogo B, García-Hurtado N, Zurita S, Martín-Trillo M, Martínez Zapater JM, Moreno V, Lozano R (2004)
    Heterologous expression of Arabidopsis APETALA 1 gene in transgenic tomato influences plant architecture and flowering time

    Molecular Breeding 13: 155-163

  • Ellul P, Rios G, Atares A, Serrano R, Moreno V (2003)
    The expressión of the Saccharomyces cerevisiae HAL1 gene increases salt tolerance in transgenic watermelon (Citrullus lanatus [Thunb] Matsun & Nakai)

    Theoretical & Applied Genetics 107: 462-469

  • Ellul P, García-Sogo B, Pineda B, Ríos G, Roig L, Moreno V (2003)
    The ploidy level of transgenic plants in Agrobacterium-mediated transformation of tomato cotyledons (Lycopersicon esculentum Mill) is genotype and procedure dependent

    Theoretical & Applied Genetics 106: 231-238

  • Ellul P, Boscaiu M, Vicente O, Moreno V, Roselló JA (2002)
    Intra- and interspecific variation in DNA content in Cistus (Cistacae)

    Annals of Botany 90: 345-351

  • Arrillaga I, Gisbert C, Sales E, Roig L, Moreno V (2001)
In vitro plant regeneration and gene transfer in the wild tomato Lycopersicon cheesmanii

Journal of Horticultural Science & Biotechnology 76: 413-418

  • Gisbert C, Rus AM, Bolarin MC, Lopez-Coronado JM, Arrillaga I, Montesinos C, Caro M, Serrano R, Moreno V (2000)
    The yeast HAL1 gene improves salt tolerance of transgenic tomato

    Plant Physiology 123: 393-402

  • Gisbert C, Arrillaga I, Roig LA, Moreno V (1999)
    Acquisition of a collection of lycopersicon pennellii (Corr. D’arcy) transgenic plants with uidA and nptii marker genes

    Journal of Horticultural Science & Biotechnology 74: 105-109

  • Serrano R, Culianz-Macia FA, Moreno V (1999)
    Genetic engineering of salt and drought tolerance with yeast regulatory genes

    Scientia Horticulturae 78: 261-269

  • Cano EA, Perez-Alfocea F, Moreno V, Caro M, Bolarin MC (1998)
    Evaluation of salt tolerance in cultivated and wild tomato species through in vitro shoot apex culture

    Plant Cell Tissue and Organ Culture 53: 19-26

  • Arrillaga I, Gil-Mascarell R, Gisbert C, Sales E, Montesinos C, Serrano R, Moreno V (1998)
    Expression of the yeast HAL2 gene in tomato increases the in vitro salt tolerance of transgenic progenies

    Plant Science 136: 219-226

  • Dabauza M, Gonzalez-Candelas L, Bordas M, Roig LA, Ramon D, Moreno V (1998)
    Regeneration and characterisation of cucumis melo l. (+) cucumis anguria l. Var. Longipes (hook. Fil.) Meeuse somatic hybrids

    Plant Cell Tissue and Organ Culture 52: 123-131

  • Bordas M, Gonzalez-Candelas L, Dabauza M, Ramon D, Moreno V (1998)
    Somatic hybridization between an albino cucumis melo l. Mutant and cucumis myriocarpus naud.

    Plant Science 132: 179-190

  • Bordas, M, Montesinos, C, Dabauza, M, Salvador A, Roig LA, Serrano R, Moreno V (1997)
    Transfer of the yeast salt tolerance gene HAL1 to cucumis melo l cultivars and in vitro evaluation of salt tolerance

    Transgenic Research 6: 41-50

  • Dabauza M, Bordas M, Salvador A, Roig LA, Moreno V (1996)
    Plant regeneration and agrobacterium-mediated transformation of cotyledon explants of citrullus colocynthis (l.) Schrad.

    Plant Cell Reports 16: 888-892

  • Cano EA, PerezAlfocea F, Moreno V, Bolarin MC (1996)
    Responses to NaCl stress of cultivated and wild tomato species and their hybrids in callus cultures

    Plant Cell Reports 15: 791-794

Projects

Generación de líneas T-DNA de Solanum lycopersicum y S. pennellii con una trampa de promotores e identificación de genes relacionados con estrés abiótico y procesos del desarrollo (MCYT / FEDER AGL2006-15290-C03-01/AGR), 01-01-2010/31-12-2012.
Entidad financiadora:  Ministerio Ciencia y Tecnología
IP:  Vicente Moreno
Identification of genes and molecules associated to tomato fruit quality and participation in the sequencing of euchromatic regions of Chr9. A genomic approach, 15-09-2005/14-09-2008.
Entidad financiadora:  Genoma España/MCYT
IP:  A Granell
Generación de líneas T-DNA de tomate y Lycopersicon cheesmanii y análisis funcional de genes relacionados con tolerancia a la salinidad (MCYT / FEDER AGL2003-07427-C02-01), 01-12-2003/30-11-2006.
Entidad financiadora: Ministerio de Ciencia y Tecnología
IP:  V Moreno
Efecto de diversos genes sobre el nivel de tolerancia a la salinidad y el estrés hídrico en plantas transgénicas de tomate (CICYT AGL2000-1680-C02-01), 31-12-2000/31-12-2003.
Entidad financiadora:  CICYT

IP:  V Moreno

Patent

Título:
Método para la obtención de cultivares de tomate con frutos sin semillas y mayor calidad organoléptica
Fecha de solicitud:
Jueves, 23 Junio, 2005
Autores:
Ellul P, Angosto T, García-Sogo B, Martín Trillo M, Martinez-Zapater JM, Lozano R, y Moreno V
Título: 
Procedimiento para modificar la arquitectura de la inflorescencia de las plantas
Fecha de solicitud: 
Martes, 13 Febrero, 2007
Autores:
Cañas LA, Beltrán JP, Medina M, Roque E, García-Sogo B, Castelblanque L, Pineda B y Moreno V
Título: 
Promotor constitutivo de Solanum lycopersicum
Fecha de solicitud:
Jueves, 24 Julio, 2008
Autores: 
Orzaez D, Hueso L, Pineda P, Anton T, Moreno V, Granell A
Título:
Nuevo cultivar con frutos y sépalos convertidos en frutos de alto interés para su consumo fresco y procesado industrial
Fecha de solicitud: 
Jueves, 18 Diciembre, 2008
Autores: 
Pineda B, Giménez-Caminero E, García-Sogo B, Capel J, Antón T, Atarés A, Angosto T, Moreno V, Lozano R

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