Dra. Stefanie Rosa. Swedish University of Agricultural Sciences.
«DNA Repair and Transcription Explored by Single-Cell and Single-Molecule Imaging«
Abstract:
I have long been fascinated by the extraordinary degree of compaction of the DNA inside the cell nucleus and the yet dynamically orchestrated organization necessary to regulate and guide complex cellular processes. Single cell/single molecule techniques avoid ensemble averaging and allow capturing previously unobtainable detail or heterogeneous behaviour, rendering them particularly powerful in elucidating mechanisms of cell function at the molecular level. In my talk, I will present how these methods have enabled us to delve deeper into the molecular mechanisms associated with DNA damage repair and transcription in plants.
Website: https://stefanierosa.wixsite.com/rosalab
References:
- Kutashev K, Meschichi A, Reeck S, Fonseca A, Sartori K, White CI, Sicard A, Rosa S* (2024). Differences in RAD51 transcriptional response and cell cycle dynamics reveal varying sensitivity to DNA damage among Arabidopsis thaliana root cell types. New Phytologist243(3): 966-980. (link)
- Meschichi A, Rosa S* (2024) Plant chromatin on the move: an overview of chromatin mobility during transcription and DNA repair. The Plant Journal,118(4):953-962. (link)
- Antoniou-Kourounioti RL, Meschichi A, Reeck S, Berry S, Menon G, Zhao Y, Fozard J, Holmes T, Wang H, Hartley M, Dean C*, Rosa S*, Howard M* (2023) Integrating analog and digital modes of gene expression at Arabidopsis FLC. eLife 2023;12:e79743. (link)
- Zhao, L., Fonseca, A., Meschichi, A., Sicard A*, Rosa S* (2023) Whole-mount smFISH allows combining RNA and protein quantification at cellular and subcellular resolution. Nature Plants;doi: 10.1038/s41477-023-01442-9. (link)
- Meschichi A, Reeck S, Sicard A, Pontvianne F, Rosa S (2022) The plant-specific DDR factor SOG1 increases chromatin mobility in response to DNA damage. EMBO Reports23:e54736. (link)
If anyone is interested in meeting with the speaker, please contact Antonio Serrano-Mislata (antserra@upv.edu.es).
