Javier Gallego Bartolomé

Me licencié como Ingeniera Agrónoma en la Universitat Politècnica de València (UPV) en 2004. Después me incorporé al laboratorio de los doctores Blázquez y Alabadí en el IBMCP (Valencia, España), donde estudié distintos mecanismos moleculares mediante los cuales la hormona vegetal giberelina (GA) interactúa con otras hormonas para controlar la expresión génica. Una vez defendida mi tesis doctoral en 2011, realicé una breve estancia posdoctoral en el laboratorio de la Dra. Chory (The Salk Institute, La Jolla, EE.UU.), y en 2013 me trasladé al laboratorio del Dr. Jacobsen (UCLA, Los Ángeles, EE.UU.), donde trabajé en la manipulación dirigida de la metilación del ADN en plantas y estudié aspectos mecanísticos de la maquinaria de silenciamiento de novo. Actualmente, en el IBMCP, mi equipo está interesado en la regulación epigenética de la expresión génica en plantas.

Selected publications:

(* first author, # corresponding author)

1. The TRIPLE PHD FINGERS proteins are required for SWI/SNF complex-mediated +1 nucleosome positioning and transcription start site determination in Arabidopsis. Diego-Martin B*,  Pérez-Alemany J*,  Candela-Ferre J,  Corbalán-Acedo A,  Pereyra J,  Alabadí D,  Jami-Alahmadi Y,  Wohlschlegel J, Gallego-Bartolomé, J#. Nucleic Acids Research, Volume 50, Issue 18, 14 October 2022, Pages 10399–10417, doi.org/10.1093/nar/gkac826
2. Comprehensive identification of SWI/SNF complex subunits underpins deep eukaryotic ancestry and reveals new plant components. Hernández-García J, Diego-Martin B, Kuo PH, Jami-Alahmadi Y, Vashisht AA, Wohlschlegel J, Jacobsen SE, Blázquez MA, Gallego-Bartolomé J#. Commun Biol. 2022 Jun 6;5(1):549. doi: 10.1038/s42003-022-03490-x.PMID: 35668117
3. Liu W*, Gallego-Bartolomé J*, Zhou Y, Zhong Z, Wang M, Wongpalee SP, Gardiner J, Feng S, Kuo PH, Jacobsen SE (2021). Ectopic targeting of CG DNA methylation in Arabidopsis with the bacterial SssI methyltransferase. Nat Commun. 2021 May 25;12(1):3130.
4. Zhong Z, Feng S, Duttke SH, Potok ME, Zhang Y, Gallego-Bartolomé J, Liu W, Jacobsen SE. (2021). DNA methylation-linked chromatin accessibility affects genomic architecture in Proc Natl Acad Sci U S A. 2021 Feb 2;118(5):e2023347118
5. Gallego-Bartolomé J# (2020). DNA methylation in plants: mechanisms and tools for targeted    manipulation. New Pythologist. doi.org/10.1111/nph.16529
6. Wongpalee SP*, Liu S*, Gallego-Bartolomé J*, Leitner A, Aebersold R, Liu W, Yen L, Nohales MA, Kuo PH, Vashisht AA, Wohlschlegel JA, Feng S, Kay SA, Zhou ZH, Jacobsen SE. (2019). CryoEM structures of Arabidopsis DDR complexes involved in RNA-directed DNA methylation. Nat Commun. 2019 Sep 2;10(1):3916. (*equal contribution)
7. Papikian A, Liu W, Gallego-Bartolomé J, Jacobsen SE. (2019). Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Nat Commun. 2019 Feb 13;10(1):729.
8. Gallego-Bartolomé J, Liu W, Kuo PH, Feng S, Ghoshal B, Gardiner J, Zhao JM, Park SY, Chory J, Jacobsen SE. (2018). Co-targeting RNA Polymerases IV and V promotes efficient de novo DNA methylation in Arabidopsis. Cell. 2019 Feb 21;176(5):1068-1082.e19.
9. Harris CJ, Scheibe M, Wongpalee SP, Liu W, Cornett EM, Vaughan RM, Li X, Chen W, Xue Y, Zhong Z, Yen L, Barshop WD, Rayatpisheh S, Gallego-Bartolome J, Groth M, Wang Z, Wohlschlegel JA, Du J, Rothbart SB, Butter F, Jacobsen SE. (2018). A DNA methylation reader complex that enhances gene transcription. Science. 2018 Dec 7;362(6419):1182-1186.
10. Gallego-Bartolomé J*, Gardiner J*, Liu W*, Papikian A*, Ghoshal B, Kuo HY, Zhao JM, Segal DJ, Jacobsen SE (2017). Targeted DNA demethylation of the Arabidopsis genome using the Human TET1 catalytic domain. Proc Natl Acad Sci U S A. Feb 27;115(9):E2125-E2134. (*equal contribution).
11. Zhong X, Du J, Hale CJ, Feng S, Gallego-Bartolome J, Vashisht A, Chory J, Wohlschlegel J, Patel D and Jacobsen SE (2014). Molecular mechanism of action of plant DRM de novo DNA methyltransferases. Cell. 2014 May 22;157(5):1050-60
12. Gallego-Bartolomé J, Minguet EG, Grau-Enguix F, Abbas M, Locascio A, Thomas SG, Alabadí D, Blázquez MA. (2012) Molecular mechanism for the interaction between gibberellin and brassinosteroid signaling pathways in Arabidopsis. Proc Natl Acad Sci U S A. Aug 14;109(33):13446-51. doi: 10.1073/pnas.1119992109. Epub 2012 Jul 30.
13. Gallego-Bartolomé J, Alabadí D, Blázquez MA. (2011) DELLA-Induced Early Transcriptional Changes during Etiolated Development in Arabidopsis thaliana. PLoS One 6(8):e23918
14. Gallego-Bartolomé J, Minguet EG, Marín JA, Prat S, Blázquez MA, Alabadí D. (2010)Transcriptional diversification and functional conservation between DELLA proteins in Arabidopsis. Mol Biol Evol. 27: 1247-1256.