“Small peptides, a game-changing strategy for synthetic biology”:
Peptide-based drugs have gained a wide range of applications in biotechnology, making necessary to explore new routes that go beyond traditional peptide identification strategies. Here we describe the construction of a combinatorial genetic-based library [8x(NNK)] able to encode millions of different cyclic peptides, at once. The library (CYCLIC) can be screened by using the GAL4-based yeast two-hybrid assay, which enables to detect protein-peptide interactions in vivo. Cyclic peptides show higher stability and permeability of biological membranes as well as higher affinity for the protein target, making them preferable to the linear counterparts. The effectiveness of the CYCLIC-based technology will be described together with some examples of its applications in plant biotechnology
Seminar Frank Takken, University of Amsterdam, The Netherlands. 17th June 12:30h
13 de June de 2022
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Identification of host targets of Fusarium effector proteins provides insight in disease resistance and susceptibility. Vascular wilt diseases are a major problem in agriculture, Fusarium
