GROUP: Plant Molecular Virology

PRINCIPALES LÍNEAS DE INVESTIGACIÓN

1. Caracterización de los genes y funciones esenciales en el ciclo infeccioso de virus pertenecientes a los grupos de los Ilar y Carmovirus que afectan de manera importante a árboles frutales y cultivos de interés agrícola.
2. Estudios sobre el movimiento intra- e intercelular de virus y viroides en sus huéspedes susceptibles.
3. Tráfico de proteínas y RNAs a través del floema.
4. Silenciamiento de RNA en el proceso de patogénesis de virus y viroides.
5. Desarrollo y mejora de nuevos métodos de diagnosis viral basados en el componente genómico de los virus.

COLABORACIONES CON EMPRESA

La profundización en el conocimiento de los mecanismos de expresión de virus que afectan a árboles frutales, a ornamentales y a especies hortícolas ha posibilitado su aplicación a resolver aspectos prácticos de ciertas virosis problemáticas para el Sector socioeconómico correspondiente. Se han establecido diversos convenios de investigación y transferencia de resultados con empresas y/o organismos públicos: Barberet y Blanc S. A., Primaflor S.A.,  FECOAM, Agromillora Catalana S.A., Laboratorio de Sanidad Vegetal-Tenerife, Laboratori de Sanitat Vegetal-Barcelona; Centro de Investigación y Tecnología Agroalimentaria de Aragón-Zaragoza, Centro Nacional de Semillas y Plantas de Vivero-Zaragoza.

• Leastro, M.O., Freitas-Astúa, J., Kitajima, E.W., Pallás, V., Sánchez-Navarro, J.A. (2021). Unravelling the involvement of cilevirus p32 protein in the viral transport. Scientific Reports 11 (1), 2943. DOI: 10.1038/s41598-021-82453-4.
• Di Serio, F., Owens, R.A., Li, S-H., Matoušek, J., Pallás, V., Randles, J.W., Sano, T., Verhoeven, J.Th.J., Vidalakis, G., Flores, R. and ICTV Report Consortium. (2021). ICTV Virus Taxonomy Profile: Pospiviroidae. Journal of General Virology 102:001543. DOI 10.1099/jgv.0.001543.
• Marquez-Molins, J., Gomez, G., Pallas, V. (2021). Hop stunt viroid: A polyphagous pathogenic RNA that has shed light on viroid–host interactions. Molecular Plant Pathology, 22 (2), 153-162. DOI: 10.1111/mpp.13022.
• Leastro, M.O., Freitas-Astúa, J., Kitajima, E.W., Pallás, V., Sánchez-Navarro, J.Á. (2021). Membrane association and topology of citrus leprosis virus c2 movement and capsid proteins. Microorganisms, 9 (2), 1-9. DOI: 10.3390/microorganisms9020418.
• Vázquez Prol, F., Márquez-Molins, J., Rodrigo, I., López-Gresa, M.P., Bellés, J.M., Gómez, G., Pallás, V. and Lisón, L. (2021). Symptom severity, infection progression and plant responses in solanum plants caused by three pospiviroids vary with the inoculation procedure. Int. J. Mol. Sci. 22, 6189. https://doi.org/10.3390/ijms22126189.
• Alvarado-Marchena, L., Marquez-Molins, J., Martinez-Perez, M., Aparicio, F. and Pallas, V. (2021). Mapping of functional subdomains in the atALKBH9B m 6 A- demethylase required for its binding to the viral RNA and to the coat protein of alfalfa mosaic virus. Front. Plant Sci. 12:701683. doi: 10.3389/fpls.2021.701683.
• Navarro, J.A., Saiz-Bonilla, M., Sanchez-Navarro, J.A. and Pallas, V. (2021). The mitochondrial and chloroplast dual targeting of a multifunctional plant viral protein modulates chloroplast-to-nucleus communication, RNA silencing suppressor activity, encapsidation, pathogenesis and tissue tropism. Plant J. 108, 197–218 doi: 10.1111/tpj.15435
• Marquez- Molins, J., Navarro, J.A., Seco, L.C., Pallas, V. and Gomez, G. (2021). Might exogenous circular RNAs act as protein-coding transcripts in plants?. RNA Biol. 18:sup1, 98-107, DOI: 10.1080/15476286.2021.1962670.
• Kuhn, J.H., Adkins, S., Agwanda, B. et al. (2021) Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Arch Virol (2021). https://doi.org/10.1007/s00705-021-05143-6.
• Martínez-Pérez M, Gómez-Mena C, Alvarado-Marchena L, Nadi R, Micol JL, Pallas V and Aparicio F (2021) The m6A RNA Demethylase ALKBH9B Plays a Critical Role for Vascular Movement of Alfalfa Mosaic Virus in Arabidopsis. Front. Microbiol. 12:745576. doi: 10.3389/fmicb.2021.745576
• Leastro, M.O., Villar-álvarez, D., Freitas-Astúa, J., Kitayima, E.W., Pallás, V., Sánchez-Navarro, J.Á. (2021). Spontaneous mutation in the movement protein of citrus leprosis virus c2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage. Viruses 13(12),2498.
• Navarro, J.A., Serra-Soriano, M., Corachán-Valencia, L., Pallás, V. (2020). A conserved motif in three viral movement proteins from different genera is required for host factor recruitment and cell-to-cell movement. Scientific Reports, 10 (1), art. no. 4758. https://doi.org/10.1038/s41598-020-61741-5.
• Salavert, F., Navarro, J.A., Owen, C.A., Khechmar, S., Pallás, V., Livieratos, I.C. (2020). Cucurbit chlorotic yellows virus p22 suppressor of RNA silencing binds single-, double-stranded long and short interfering RNA molecules in vitro. Virus Research, 279, art. no. 197887.
• Salgado-Ortíz, H., De La Torre-Almaraz, R., Sánchez-Navarro, J.Á., Pallás, V. (2020). Identification and genomic characterization of a novel tobamovirus from prickly pear cactus (2020) Archives of Virology, 165 (3), 781-784.
• López-González, S., Navarro, J.A., Pacios, L.F., Sardaru, P., Pallás, V., Sánchez, F. and Ponz, F. (2020). Association between flower stalk elongation, an Arabidopsis developmental trait, and the subcellular location and movement dynamics of the viral non-structural protein P3. Mol. Plant Pathol. 21, 1271–1286.
• Leastro MO, Castro DYO, Freitas-Astúa J, Kitajima EW, Pallás V and Sánchez-Navarro JÁ (2020). Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity. Front. Microbiol. 11, 1231. doi: 10.3389/fmicb.2020.01231.
• Leastro, M.O., Freitas-Astúa, J., Kitajima, E.W., Pallás, V., Sánchez-Navarro, J.A. (2020). Dichorhaviruses Movement Protein and Nucleoprotein Form a Protein Complex That May Be Required for Virus Spread and Interacts in vivo With Viral Movement-Related Cilevirus Proteins. Front. Microbiol. 11, e571807. DOI: 10.3389/fmicb.2020.571807.
• Kuhn, J.H., Pallas, V., et al. (2020) (196 authors). Taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Arch. Virol. 165 (12), 3023-3072.
• Martínez-Pérez, M., Navarro, J.A., Pallás, V. and Sánchez-Navarro, J.A (2019)
  A sensitive and rapid RNA silencing suppressor activity assay based on alfalfa mosaic virus expression vector.
  Virus Research 272, 1977332

• Marquez‑Molins, J., Navarro, J.A., Pallas V. and Gomez, G. (2019)
  Highly efficient construction of infectious viroid‑derived clones
  Plant Methods 15:87

  ---

• Bujarski, J., Gallitelli, D., García-Arenal, F., Pallás, V., Palukaitis, P., Reddy, M.K., Wang, A (2019)
  ICTV Virus Taxonomy Profile: Bromoviridae
  Journal of General Virology 100 (8): 1206-1207.

  ---
• Navarro, J.A., Sanchez-Navarro, J.A., Pallas, V (2019)
  Key checkpoints in the movement of plant viruses through the host.
  Advances in Virus Research, 104: 1-64.

  ---
• De La Torre-Almaráz, R., Pallás, V., Sánchez-Navarro, J.A (2019)
  Molecular characterization of a new trichovirus from peach in Mexico
  Arch. Virol. 164:2617–2620

  ---
• Sánchez-Navarro, J.Á., Corachán, L., Font, I., Alfaro-Fernández, A., Pallás, V (2019)
  Polyvalent detection of twelve viruses and four viroids affecting tomato by using a unique polyprobe
  Eur. J. Plant Pathol. 155:361–368.

  ---
• Di Serio, F., Li, S.-F., Matoušek, J., Owens, R.A., Pallás, V., Randles, J.W., Sano, T., Verhoeven, J.T.J., Vidalakis, G., Flores, R., Lefkowitz, E., Davison, A., Siddell, S., Simmonds, P., Sabanadzovic, S., Smith, D.B., Orton, R. and Zerbini, F.M. (ICTV Report Consortium). (2018)
  ICTV virus taxonomy profile: Avsunviroidae
  Journal of General Virology 99 (5): 611-612.

  ---
• Rubio, M., Martínez-Gómez, P., Pallás, V., Dicenta, F., Sánchez-Navarro, J.A. (2018)
  Molecular characterization of ilarvirus Prunus necrotic ring spot virus (PNRSV) and Prune dwarf virus (PDV) in Spanish autochthonous almond cultivars.
  Acta Horticulturae 1219: 353-356.

  ---
• Aparicio, F., Aramburu, J., Herranz, M.C., Pallás, V., López, C (2018)
  Parietaria mottle virus: A potential threat for tomato crops?.
  Acta Horticulturae 1207: 261-268.

  ---