Abscisic acid (ABA) plays a crucial role to integrate plant response to abiotic stress (particularly drought and salinity) into the regulation of plant growth and development. An increase in ABA levels and the subsequent plant response to the hormone are key components of the adaptive mechanism to resist/avoid those forms of abiotic stress. Accordingly, the characterization of ABA signaling offers a high biotechnological potential to improve plant tolerance to drought and salinity.
Because of its essential function in plant stress physiology, elucidating the abscisic acid (ABA) signaling pathway holds enormous promise for application in agriculture. A breakthrough in ABA signaling occurred in 2009, i.e. the discovery of the 14-member PYR/PYL/RCAR family of ABA receptors (Park et al., 2009; Ma et al., 2009; Santiago et al., 2009). Control of ABA signaling by PYR/PYL/RCAR ABA-receptors involves direct ABA-dependent inhibition of clade A phosphatases type-2C (PP2Cs), for instance, ABI1, HAB1, PP2CA, which are key negative regulators of the pathway (Saez et al., 2006; Rubio et al., 2009). Inhibition of PP2Cs leads to activation of sucrose non-fermenting 1-related subfamily 2 (SnRK2) kinases, which regulate stomatal aperture and transcriptional response to ABA. Thus, a core signaling network for ABA has emerged from these findings (Fujii et al., 2009; Cutler et al., 2010).
Agrochemicals: Crystal structures are available for ABA receptors and receptor-ABA-phosphatase complexes, which reveal key details on the mode of interaction between the receptor, the hormone, and the protein phosphatase as well as the mechanism of activation of ABA signaling. This information will be used in the structure-assisted identification of synthetic molecules able to act as agonists of ABA receptors and activate ABA signaling in plants. These molecules might have the potential to improve the yield of crop plants under drought stress or any other properties modulated by the ABA pathway in crop or ornamental plants. To this end, we will clone and produce recombinant ABA receptors in tomato, orange, grapevine, and monocots as targets for screening small molecules capable of acting as agrochemicals through the activation of ABA receptors. Additionally, through collaboration with organic chemists and protein crystallographers, direct synthesis of small molecules that fit into the ABA binding ligand pocket has been performed. Recently, we achieved the structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought, protected by the EP21382948 patent.
Molecular genetics: The ABA signaling group has played a key role in the discovery and characterization of the PYR/PYL/RCAR family of ABA receptors, and their connection with PP2Cs and SnRK2s (Rodriguez et al., 1998; Gonzalez-Guzman et al., 2002; Saez et al., 2004, 2006, 2008; Park et al., 2009; Santiago et al., 2009a, 2009b; Rubio et al., 2009; Vlad et al., 2009; Fujii et al., 2009; Cutler et al., 2010; Vlad et al., 2010; Dupeux et al., 2011a, 2011b; Antoni et al. 2012; Gonzalez-Guzman et al., 2012; Antoni et al., 2013; Merilo et al., 2013; Pizzio et al., 2013).
Some of these works are landmarks in ABA signaling and together with other results have brought about a breakthrough in our knowledge of the pathway. Later on, we provided insight into the subcellular location of ABA receptors and we discovered that C2-domain abscisic acid-related proteins mediate the interaction of PYR/PYL/RCAR receptors with the plasma membrane (Rodriguez et al., 2014; Diaz et al., 2016). Concerning ABA-induced transcriptional regulation, we have discovered a link between SWI/SNF chromatin remodeling complexes and core components of ABA signaling (Saez et al., 2008; Han et al., 2012; Peirats-Llobet et al., 2016). We have contributed several genetic strategies that enhance ABA signaling as a valuable tool for improving plant water use. Among them, the constitutive inactivation of PP2Cs (Saez et al., 2006), overexpression of monomeric ABA receptors (Santiago et al., 2009a; Gonzalez-Guzman et al., 2014), and the generation of mutated ABA receptors that enhance ABA-dependent inhibition of PP2Cs (Pizzio et al., 2013). As a result, three patents were filled to cover these findings.
We have played a pioneering role in studies that address the turnover of core ABA signaling components, particularly ABA receptors and PP2Cs (Bueso et al., 2014; Irigoyen et al., 2014; Wu et al., 2016; Belda-Palazon et al., 2016, 2018 and 2019; Fernandez et al., 2020; Coego et al., 2021). We uncovered the unique role of PYL8 in root ABA signaling, which involves a non-cell-autonomous mechanism like mobile transcription factors, ABA-induced stabilization, and predominant nuclear localization (Belda-Palazon et al., 2018). We have further studied the mechanisms that affect subcellular localization and half-life of ABA receptors. We have uncovered a novel route for endosomal degradation of ABA receptors through the ESCRT pathway (Belda-Palazón et al., 2016; Yu et al., 2016; Garcia-Leon et al., 2019) and we have identified two novel families of E3 ligases that mediate the turnover of PP2Cs (Wu et al., 2016; Belda-Palazon et al., 2019; Julian et al., 2019). Physiological studies and structural biology of crop ABA receptors are a long-lasting interest of our group (Gonzalez-Guzman et al., 2014), to elucidate the formation of receptor-ABA-phosphatase complexes (Moreno-Alvero et al., 2017) and the key role of PYL8-like receptors in crop response to abiotic stress (Garcia-Maquilon et al., 2021; Pizzio et al., 2022). International collaborations (4 publications in Nature Plants, 1 Science Advances, 1 Dev Cell) have provided key findings on the role of SnRK2s-PP2Cs-ABA receptors in root hydrotropism (Dietrich et al., 2017; Miao et al., 2021), regulation of plant growth via SnRK1 and TOR (Belda-Palazon et al., 2020), specific roles of ABA receptors in stomatal response to ABA and high CO2 (Dittrich et al., 2019) and moonlight roles of FYVE1/FREE1 in repression of ABA signaling (Li et al., 2019).
Eckardt,N.A., Avin-Wittenberg,T., Bassham,D.C., Chen,P., Chen,Q., Fang,J., Genschik,P., Ghifari,A.S., Guercio,A.M., Gibbs,D.J., Heese,M., Jarvis,R.P., Michaeli,S., Murcha,M.W., Mursalimov,S., Noir,S., Palayam,M., Peixoto,B., Rodriguez,P.L., Schaller,A., Schnittger,A., Serino,G., Shabek,N., Stintzi,A., Theodoulou,F.L., Üstün,S., van Wijk,K.J., Wei,N., Xie,Q., Yu,F. and Zhang,H. (2024). The lowdown on breakdown: Open questions in plant proteolysis.
Plant Cell 2024 Jul 9:koae193. doi: 10.1093/plcell/koae193
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Sanchez-Olvera, M., Martin-Vasquez, C., Mayordomo, C., Illescas-Miranda, J., Bono, M., Coego, A., Alonso, J., Hernández-González, M., Jiménez-Arias, D., Forment, J., Albert, A., Granell, A., Borges, A. A., Rodriguez, P. L* (2024). ABA-receptor agonist iSB09 decreases soil water consumption and increases tomato CO2 assimilation and water use efficiency under drought stress.
Environmental and Experimental Botany 225, 105847.https://doi.org/10.1016/j.envexpbot.2024.105847
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Morales-Sierra,S., Luis,J.C., Jimenez-Arias,D., Rancel-Rodriguez,N.M., Coego,A., Rodriguez,P.L., Cueto,M. and Borges,A.A. (2023). Biostimulant activity of Galaxaura rugosa seaweed extracts against water deficit stress in tomato seedlings involves activation of ABA signaling.
Front Plant Sci. 14, 1251442. doi: 10.3389/fpls.2023.1251442
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Jiménez-Arias D, Morales-Sierra S, Suárez E, Lozano-Juste J, Coego A, Estevez JC, Borges AA, Rodriguez PL* (2023). Abscisic acid mimic-fluorine derivative 4 (AMF4) alleviates water deficit stress by regulating ABA-responsive genes, proline accumulation, CO2 assimilation, water use efficiency and better nutrient uptake in tomato plants.
Front Plant Sci. 14:1191967. doi: 10.3389/fpls.2023.1191967
Yang C, Li X, Chen S, Liu C, Yang L, Li K, Liao J, Zheng X, Li H, Li Y, Zeng S, Zhuang X, Rodriguez PL, Luo M, Wang Y, Gao C. (2023).ABI5-FLZ13 Module Transcriptionally Represses Growth-related Genes to Delay Seed Germination in Response to ABA.
Plant Commun. 2023 Jun 9:100636. doi: 10.1016/j.xplc.2023.100636.
Lozano-Juste J, Infantes L, Garcia-Maquilon I, Ruiz-Partida R, Merilo E, Benavente JL, Velazquez-Campoy A, Coego A, Bono M, Forment J, Pampín B, Destito P, Monteiro A, Rodríguez R, Cruces J, Rodriguez PL*, Albert A* (2023). Structure-guided engineering of a receptor-agonist pair for inducible activation of the ABA adaptive response to drought.
Sci Adv. 2023 Mar 10;9(10):eade9948. doi: 10.1126/sciadv.ade9948.
Hirt H, Al-Babili S, Almeida-Trapp M, Martin A, Aranda M, Bartels D, Bennett M, Blilou I, Boer D, Boulouis A, Bowler C, Brunel-Muguet S, Chardon F, Colcombet J, Colot V, Daszkowska-Golec A, Dinneny JR, Field B, Froehlich K, Gardener CH, Gojon A, Gomès E, Gomez-Alvarez EM, Gutierrez C, Havaux M, Hayes S, Heard E, Hodges M, Alghamdi AK, Laplaze L, Lauersen KJ, Leonhardt N, Johnson X, Jones J, Kollist H, Kopriva S, Krapp A, Masson ML, McCabe MF, Merendino L, Molina A, Moreno Ramirez JL, Mueller-Roeber B, Nicolas M, Nir I, Orduna IO, Pardo JM, Reichheld JP, Rodriguez PL, Rouached H, Saad MM, Schlögelhofer P, Singh KA, De Smet I, Stanschewski C, Stra A, Tester M, Walsh C, Weber APM, Weigel D, Wigge P, Wrzaczek M, Wulff BBH, Young IM (2023). PlantACT! – how to tackle the climate crisis.
Trends Plant Sci. 2023 28(5):537-543. doi: 10.1016/j.tplants.2023.01.005.
Chen W, Zhou H, Xu F, Yu M, Coego A, Rodriguez L, Lu Y, Xie Q, Fu Q, Chen J, Xu G, Wu D, Li X, Li X, Jaillais Y, Rodriguez PL, Zhu S, Yu F. (2023). CAR modulates plasma membrane nano-organization and immune signaling downstream of RALF1-FERONIA signaling pathway
New Phytol. 2023 Mar;237(6):2148-2162. doi: 10.1111/nph.18687
Pizzio GA, Rodriguez PL* (2022). Dual regulation of SnRK2 signaling by Raf-like MAPKKKs
Mol Plant. 2022 Aug 1;15(8):1260-1262. doi: 10.1016/j.molp.2022.07.002.
Infantes L, Rivera-Moreno M, Daniel-Mozo M, Benavente JL, Ocaña-Cuesta J, Coego A, Lozano-Juste J, Rodriguez PL, Albert A (2022). Structure-Based Modulation of the Ligand Sensitivity of a Tomato Dimeric Abscisic Acid Receptor Through a Glu to Asp Mutation in the Latch Loop.
Front Plant Sci. 2022 Jun 6;13:884029. doi: 10.3389/fpls.2022.884029. eCollection 2022.
Franco-Aragón D, García-Maquilón I, Manicardi A, Rodríguez PL, Lozano-Juste J (2022). Evaluation of the Anti-transpirant Activity of ABA Receptor Agonists in Monocot and Eudicot Plants.
Methods Mol Biol. 2022;2494:229-238. doi: 10.1007/978-1-0716-2297-1_16.
Garcia-Maquilon I, Lozano-Juste J, Alrefaei AF, Rodriguez PL* (2022). Hydrotropism: Analysis of the Root Response to a Moisture Gradient.
Methods Mol Biol. 2022;2494:17-24. doi: 10.1007/978-1-0716-2297-1_2.
Pizzio GA, Mayordomo C, Lozano-Juste J, Garcia-Carpintero V, Vazquez-Vilar M, Nebauer SG, Kaminski KP, Ivanov NV, Estevez JC, Rivera-Moreno M, Albert A, Orzaez D, Rodriguez PL* (2022). PYL1- and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue.
Cells. 2022 Feb 24;11(5):795. doi: 10.3390/cells11050795.
Belda-Palazón B, Rodriguez PL (2022). Microscopic Imaging of Endosomal Trafficking of ABA Receptors.
Methods Mol Biol. 2022;2462:59-69. doi: 10.1007/978-1-0716-2156-1_5
Julian J, Coego A, Alrefaei AF, Rodriguez PL* (2022). Affinity Purification of Ubiquitinated Proteins Using p62-Agarose to Assess Ubiquitination of Clade A PP2Cs.
Methods Mol Biol. 2022;2462:45-57. doi: 10.1007/978-1-0716-2156-1_4.
Miao R, Russinova E, Rodriguez PL* (2022). Tripartite hormonal regulation of plasma membrane H+-ATPase activity.
Trends Plant Sci Jun;27(6):588-600 https://doi.org/10.1016/j.tplants.2021.12.011
Coego A, Julian J, Lozano-Juste J, Pizzio GA, Alrefaei AF, Rodriguez PL* (2021). Ubiquitylation of ABA receptors and protein phosphatase 2C coreceptors to modulate ABA signaling and stress response.
Int. J. Mol. Sci 22, 7103. https://doi.org/10.3390/ijms22137103. Special Issue “Ubiquitylation in Plant Developmental and Physiological Processes”.
Zeng Y, Verstraeten I, Trinh HK, Heugebaert T, Stevens CV, Garcia-Maquilon I, Rodriguez PL, Vanneste S, Geelen D. (2021). Arabidopsis hypocotyl adventitious root formation is suppressed by ABA signaling
Genes 12, 1141. https://doi.org/10.3390/genes12081141. Special Issue “Root Development and Architecture in Relation to Environmental Conditions”.
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García-Maquilón I, Rodriguez PL, Vaidya AS, Lozano-Juste J. (2021)
A luciferase reporter assay to identify chemical activators of ABA signaling
Methods Mol Biol. 2021;2213:113-121
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Lozano-Juste J, García-Maquilón I, Brea J, Piña R, Albert A, Rodriguez PL, Loza MI. (2021)
Identification of ABA receptor agonists using a multiplexed high-throughput chemical screening
Methods Mol Biol. 2021;2213:99-111
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Miao R, Yuan W, Wang Y, Garcia-Maquilon I, Dang X, Li Y, Zhang J, Zhu Y, Rodriguez PL, Xu W. (2021)
Low ABA concentration promotes root growth and hydrotropism through relief of ABA INSENSITIVE 1-mediated inhibition of plasma membrane H+-ATPase 2
Science Advances 7 (12): eabd4113.
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Garcia-Maquilon,I., Coego,A., Lozano-Juste,J., Messerer,M., de Ollas,C., Julian,J., Ruiz-Partida,R., Pizzio,G., Belda-Palazon,B., Gomez-Cadenas,A., Mayer,K.F.X., Geiger,D., Alquraishi,S.A., Alrefaei,A.F., Ache,P., Hedrich,R. and Rodriguez,P.L* (2021)
PYL8 ABA receptors of Phoenix dactylifera play a crucial role in response to abiotic stress and are stabilized by ABA.
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Belda-Palazon,B., Adamo,M., Valerio,C., Ferreira,L.J., Confraria,A., Reis-Barata,D., Rodrigues,A., Meyer,C., Rodriguez,P.L. and Baena-Gonzalez,E. (2020)
A dual function of SnRK2 kinases in the regulation of SnRK1 and plant growth.
Nature Plants 6(11):1345-1353
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Belda-Palazon, B., and Rodriguez, P. L.* (2020)
Degradation of abscisic acid receptors through the endosomal pathway
Methods Mol Biol. 2177:35-48
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Lozano-Juste,J., Garcia-Maquilon,I., Ruiz-Partida,R. and Rodriguez,P.L. (2020)
Drug Discovery for Thirsty Crops
Trends Plant Sci. Sep;25(9):844-846.
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Lozano-Juste,J., Alrefaei,A.F. and Rodriguez,P.L.* (2020)
Plant Osmotic Stress Signaling: MAPKKKs Meet SnRK2s
Trends Plant Sci. Dec;25(12):1179-1182
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Fernandez, M. A., Belda-Palazon, B., Julian, J., Coego, A., Lozano-Juste, J., Inigo, S., Rodriguez, L., Bueso, E., Goossens, A., and Rodriguez, P. L.* (2020)
RBR-type E3 ligases and the Ub-conjugating enzyme UBC26 regulate ABA receptor levels and signaling
Plant Physiol. 182(4):1723-1742
- Garcia-Andrade,J., Gonzalez,B., Gonzalez-Guzman,M., Rodriguez,P.L. and Vera,P. (2020)
The Role of ABA in Plant Immunity is Mediated through the PYR1 Receptor
Int. J. Mol. Sci. 21, E5852
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Belda-Palazon, B., Julian, J., Coego, A., Wu, Q., Zhang, X., Batistic, O., Alquraishi, S. A., Kudla, J., An, C., and Rodriguez, P. L (2019)
ABA inhibits myristoylation and induces shuttling of the RGLG1 E3 ligase to promote nuclear degradation of PP2CA
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Pedro L. Rodriguez, Jorge Lozano-Juste and Armando Albert (2019)
PYR/PYL/RCAR ABA receptors
Advances in Botanical Research, Abscisic Acid in Plants 92, pages 51-82. Edited by M. Seo and A. Marion-Poll. Elsevier. doi:10.1016/bs.abr.2019.05.003
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Fernandez, M. A., Belda-Palazon, B., Julian, J., Coego, A., Lozano-Juste, J., Inigo, S., Rodriguez, L., Bueso, E., Goossens, A., and Rodriguez, P. L (2019)
RBR-type E3 ligases and the Ub-conjugating enzyme UBC26 regulate ABA receptor levels and signaling
Plant Physiol. DOI:10.1104/pp.19.00898
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Garcia-Leon, M., Cuyas, L., El-Moneim, D., Rodriguez, L., Belda-Palazon, B., Fernandez, Y., Roux, B., Zamarreño, A.M., Garcia-Mina, J.M., Nussaume, L., Rodriguez, P.L., Paz-Ares, J., Leonhardt, N., and Rubio, V. (2019)
Stomatal aperture and turnover of ABA receptors are regulated by Arabidopsis ALIX
Plant Cell 31(10):2411-2429
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Lozano-Juste, J., Masi, M., Cimmino, A., Clement, S., Fernandez, M. A., Antoni, R., Meyer, S., Rodriguez, P. L., and Evidente, A (2019)
The fungal sesquiterpenoid pyrenophoric acid B uses the plant ABA biosynthetic pathway to inhibit seed germination
J.Exp.Bot. 70(19):5487-5494
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Julian, J., Coego, A., Lozano-Juste, J., Lechner, E., Wu, Q., Zhang, X., Merilo, E., Belda-Palazon, B., Park, S. Y., Cutler, S. R., An, C., Genschik, P., and Rodriguez, P. L (2019)
The MATH-BTB BPM3 and BPM5 subunits of Cullin3-RING E3 ubiquitin ligases target PP2CA and other clade A PP2Cs for degradation
Proc.Natl.Acad.Sci.U.S.A. 116(31):15725-15734
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Li, H., Li, Y., Zhao, Q., Li, T., Wei, J., Li, B., Shen, W., Yang, C., Zeng, Y., Rodriguez, P. L., Zhao, Y., Jiang, L., Wang, X., and Gao, C. (2019)
The plant ESCRT component FREE1 shuttles to the nucleus to attenuate abscisic acid signalling
Nature Plants. 5(5):512-524
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Dittrich, M., Mueller, H. M., Bauer, H., Peirats-Llobet, M., Rodriguez, P. L., Geilfus, C. M., Carpentier, S. C., Al Rasheid, K. A. S., Kollist, H., Merilo, E., Herrmann, J., Muller, T., Ache, P., Hetherington, A. M., and Hedrich, R (2019)
The role of Arabidopsis ABA receptors from the PYR/PYL/RCAR family in stomatal acclimation and closure signal integration
Nature Plants 5(9), 1002-1011.
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Forster, S., Schmidt, L. K., Kopic, E., Anschutz, U., Huang, S., Schlucking, K., Koster, P., Waadt, R., Larrieu, A., Batistic, O., Rodriguez, P. L., Grill, E., Kudla, J., and Becker, D (2019)
Wounding-induced stomatal closure requires jasmonate-mediated activation of GORK K+ channels by a Ca2+ sensor-kinase CBL1-CIPK5 complex
Developmental Cell 48(1):87-99.e6
- Belda-Palazon, B., Gonzalez-Garcia, M. P., Lozano-Juste, J., Coego, A., Antoni, R., Julian, J., Peirats-Llobet, M., Rodriguez, L., Berbel, A., Dietrich, D., Fernandez, M. A., Madueno, F., Bennett, M. J., and Rodriguez, P. L (2018)
PYL8 mediates ABA perception in the root through non-cell-autonomous and ligand-stabilization-based mechanisms
Proc.Natl.Acad.Sci. USA 115, E11857-E11863
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Orman-Ligeza B, Morris EC, Parizot B, Lavigne T, Babé A, Ligeza A, Klein S, Sturrock C, Xuan W, Novák O, Ljung K, Fernandez MA, Rodriguez PL, Dodd IC, De Smet I, Chaumont F, Batoko H, Périlleux C, Lynch JP, Bennett MJ, Beeckman T, Draye X. (2018)
Xerobranching Response Represses Lateral Root Formation When Roots Are Not in Contact With Water
Current Biology Oct 8;28(19):3165-3173.e5
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Arbona,V., Zandalinas,S.I., Manzi,M., Gonzalez-Guzman,M., Rodriguez,P.L. and Gomez-Cadenas,A (2017)
Depletion of abscisic acid levels in roots of flooded Carrizo citrange (Poncirus trifoliata L. Raf. x Citrus sinensis L. Osb.) plants is a stress-specific response associated to the differential expression of PYR/PYL/RCAR receptors
Plant Mol. Biol. 93, 623-640.
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Pizzio, G.A. and Rodriguez, P.L (2017)
In-gel-kinase assay
Bioprotocols, Vol 7, 1-12
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Ling,Y., Alshareef,S., Butt,H., Lozano-Juste,J., Li,L., Galal,A.A., Moustafa,A., Momin,A.A., Tashkandi,M., Richardson,D.N., Fujii,H., Arold,S., Rodriguez,P.L., Duque,P. and Mahfouz,M.M (2017)
Pre-mRNA splicing repression triggers abiotic stress signaling in plants
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Dietrich,D., Pang,L., Kobayashi,A., Fozard,J.A., Boudolf,V., Bhosale,R., Antoni,R., Nguyen,T., Hiratsuka,S., Fujii,N., Miyazawa,Y., Bae,T.W., Wells,D.M., Owen,M.R., Band,L.R., Dyson,R.J., Jensen,O.E., King,J.R., Tracy,S.R., Sturrock,C.J., Mooney,S.J., Roberts,J.A., Bhalerao,R.P., Dinneny,J.R., Rodriguez,P.L., Nagatani,A., Hosokawa,Y., Baskin,T.I., Pridmore,T.P., De Veylder,L., Takahashi,H. and Bennett,M.J (2017)
Root hydrotropism is controlled via a cortex-specific growth mechanism
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Moreno-Alvero,M., Yunta,C., Gonzalez-Guzman,M., Lozano-Juste,J., Benavente,J.L., Arbona,V., Menendez,M., Martinez-Ripoll,M., Infantes,L., Gomez-Cadenas,A., Rodriguez,P.L. and Albert,A (2017)
Structure of Ligand-Bound Intermediates of Crop ABA Receptors Highlights PP2C as Necessary ABA Co-receptor
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Vicente,J., Mendiondo,G.M., Movahedi,M., Peirats-Llobet,M., Juan,Y.T., Shen,Y.Y., Dambire,C., Smart,K., Rodriguez,P.L., Charng,Y.Y., Gray,J.E. and Holdsworth,M.J (2017)
The Cys-Arg/N-End Rule Pathway Is a General Sensor of Abiotic Stress in Flowering Plants
Current Biology 27, 3183-3190
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Peirats-Llobet,M; Han,SK; Gonzalez-Guzman,M; Jeong,CW; Rodriguez,L,; Belda-Palazon,B; Wagner,D; Rodriguez,PL (2016)
A direct link between abscisic acid sensing and the chromatin remodeling ATPase BRAHMA via core ABA signaling pathway components
Molecular Plant Jan 4;9(1):136-47
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Peirats-Llobet M, Han SK, Gonzalez-Guzman M, Jeong CW, Rodriguez L, Belda-Palazon B, Wagner D, Rodriguez PL (2016)
A direct link between abscisic acid sensing and the chromatin remodeling ATPase BRAHMA via core ABA signaling pathway components
Molecular Plant 9: 136-147
- Abscisic acid catabolism generates phaseic acid, a molecule able to activate a subset of ABA receptors
- Molecular Plant 9, 1448-1450.
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Pedro L. Rodriguez y Jose M. Pardo (2016)
Biotecnología agrícola para mejorar la tolerancia a sequía y salinidad
Boletín de la Sociedad Española de Bioquímica y Biología Molecular Junio, 188, 21-24
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Diaz,M; Sanchez-Barrena,MJ; Gonzalez-Rubio,JM; Rodriguez,L; Fernandez,D; Antoni,R; Yunta,C; Belda-Palazon,B; Gonzalez-Guzman,M; Peirats-Llobet,M; Menendez,M; Boskovic,J; Marquez,J.A; Rodriguez,PL; Albert,A (2016)
Calcium-dependent oligomerization of CAR proteins at cell membrane modulates ABA signaling
Proc. Natl. Acad. Sci. U. S. A Jan 19;113(3):E396-405
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Diaz M, Sanchez-Barrena MJ, Gonzalez-Rubio JM, Rodriguez L, Fernandez D, Antoni R, Yunta C, Belda-Palazon B, Gonzalez Guzman M, Peirats-Llobet M, Menendez M, Boskovic J, Marquez JA, Rodriguez Pl, Albert A (2016)
Calcium-dependent oligomerization of CAR proteins at cell membrane modulates ABA signaling
Procedings of the National Academy of Sciences of the United States of America 113: E396-E405
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Yu,F., Lou,L., Tian,M., Li,Q., Ding,Y., Cao,X., Wu,Y., Belda-Palazon,B., Rodriguez,P.L., Yang,S. and Xie,Q (2016)
ESCRT-I component VPS23A affects ABA signaling by recognizing ABA receptors for endosomal degradation
Molecular Plant 9, 1570-1582
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Chen J, Yu F, Liu Y, Du C, Li X, Zhu S, Wang X, Lan W, Rodriguez PL, Liu X, Li D, Chen L, Luan S (2016)
FERONIA interacts with ABI2-type phosphatases to facilitate signaling cross-talk between abscisic acid and RALF peptide in Arabidopsis
Proc. Natl. Acad. Sci. U. S. A pii: 201608449
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Chen,J., Yu,F., Liu,Y., Du,C., Li,X., Zhu,S., Wang,X., Lan,W., Rodriguez,P.L., Liu,X., Li,D., Chen,L. and Luan,S (2016)
FERONIA interacts with ABI2-type phosphatases to facilitate signaling cross-talk between abscisic acid and RALF peptide in Arabidopsis
Proc. Natl. Acad. Sci. U. S. A Sep 13;113(37):E5519-27
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Belda-Palazon B, Rodriguez L, Fernandez MA, Castillo MC, Anderson EA, Gao C, Gonzalez-Guzman M, Peirats-Llobet M, Zhao Q, De Winne N, Gevaert K, De Jaeger G, Jiang L, Leon J, Mullen RT, Rodriguez PL (2016)
FYVE1/FREE1 Interacts with the PYL4 ABA Receptor and Mediates its Delivery to the Vacuolar Degradation Pathway
Plant Cell pii: tpc.00178.2016
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Belda-Palazon,B., Rodriguez,L., Fernandez,M.A., Castillo,M.C., Anderson,E.A., Gao,C., Gonzalez-Guzman,M., Peirats-Llobet,M., Zhao,Q., De Winne,N., Gevaert,K., De Jaeger,G., Jiang,L., Leon,J., Mullen,R.T. and Rodriguez,P.L. (2016)
FYVE1/FREE1 Interacts with the PYL4 ABA Receptor and Mediates its Delivery to the Vacuolar Degradation Pathway
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Pedro L. Rodriguez (2016)
Grupo de Señalización y Biosíntesis del ABA
Boletín de la Sociedad Española de Fisiología Vegetal Julio, 63, 43-50
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Anton R, Dietrich D, Bennett MJ, Rodriguez PL (2016)
Hydrotropism: Analysis of the Root Response to a Moisture Gradient
Methods Mol. Biol 1398: 3-9
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Antoni,R., Dietrich,D., Bennett,M.J. and Rodriguez,P.L. (2016)
Hydrotropism: Analysis of the Root Response to a Moisture Gradient.
Methods Mol. Biol. 1398, 3-9.
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Krzywinska,E., Bucholc,M., Kulik,A., Ciesielski,A., Lichocka,M., Debski,J., Ludwikow,A., Dadlez,M., Rodriguez,P.L. and Dobrowolska,G (2016)
Phosphatase ABI1 and okadaic acid-sensitive phosphoprotein phosphatases inhibit salt stress-activated SnRK2.4 kinase
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Krzywińska E, Bucholc M, Kulik A, Ciesielski A, Lichocka M, Dębski J, Ludwików A, Dadlez M, Rodriguez PL, Dobrowolska G (2016)
Phosphatase ABI1 and okadaic acid-sensitive phosphoprotein phosphatases inhibit salt stress-activated SnRK2.4 kinase.
BMC Plant Biology 16: 136
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Ling Y, Alshareef S, Butt H, Lozano-Juste J, Li L, Galal A, Moustafa A, Ahmad Momin A, Tashkandi M, Richardson D, Fujii H, Arold S, Rodriguez PL, Duque P, Mahfouz M (2016)
Pre-mRNA splicing repression triggers abiotic stress signaling in plants
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Krzywinska,E., Kulik,A., Bucholc,M., Fernandez,M.A., Rodriguez,P.L. and Dobrowolska,G (2016)
Protein phosphatase type 2C PP2CA together with ABI1 inhibits SnRK2.4 activity and regulates plant responses to salinity
Plant Signal. Behav. 11, e1253647
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Wu Q, Zhang X, Peirats-Llobet M, Belda-Palazon B, Wang X, Cui S, Yu X, Rodriguez PL, An C (2016)
Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA
Plant Cell pii: tpc.00364.2016
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Wu,Q., Zhang,X., Peirats-Llobet,M., Belda-Palazon,B., Wang,X., Cui,S., Yu,X., Rodriguez,P.L and An,C (2016)
Ubiquitin Ligases RGLG1 and RGLG5 Regulate Abscisic Acid Signaling by Controlling the Turnover of Phosphatase PP2CA
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DiazM, Sanchez-Barrena MJ, Gonzalez-Rubio JM, Rodriguez L, Fernandez D, Antoni R, Yunta C, Belda-Palazon B, Gonzalez-Guzman M, Peirats-Llobet M, Menendez M, Boskovic J, Marquez JA, Rodriguez PL, Albert A (2015)
Calcium-dependent oligomerization of CAR proteins at cell membrane modulates ABA signaling
Proceedings of the National Academy of Sciences USA 113: E396-E405
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Antoni R, Dietrich D, Bennett MJ, Rodriguez PL (2015)
Hydrotropism: analysis of the root response to a moisture gradient
Methods in Molecular Biology 1398: 3-9
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Castillo MC, Lozano-Juste J, González-Guzmán M, Rodriguez L, Rodriguez PL, León J (2015)
Inactivation of PYR/PYL/RCAR ABA receptors by tyrosine nitration may enable rapid inhibition of ABA signaling by nitric oxide in plants
Science Signaling 8: ra89
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Amiguet-Vercher A, Santuari L, Gonzalez-Guzman M, Depuydt S, Rodriguez P, Hardtke CS (2015)
The IBO germination quantitative trait locus encodes a phosphatase 2C-related variant with a nonsynonymous amino acid change that interferes with abscisic acid signaling
New Phytologist 205: 1076-1082
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Rodriguez PL, Lozano-Juste J (2015)
Unnatural agrochemical ligands for engineered abscisic acid receptors
Trends in Plant Science 20: 330-332
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Bueso E, Ibañez C, Sayas E, Muñoz-Bertomeu J, Gonzalez-Guzman M, Rodriguez PL, Serrano R (2014)
A forward genetic approach in Arabidopsis thaliana identifies a RING-type ubiquitin ligase as a novel determinant of seed longevity
Plant Science 215–216: 110–116
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Rodriguez L, Gonzalez-Guzman M, Diaz M, Rodrigues A, Peirats-Llobet M, Izquierdo-Garcia AC, Antoni R, Marquez JA, Mulet JM, Albert A, Rodriguez PL (2014)
C2-domain abscisic acid-related proteins mediate the interaction of PYR/PYL/RCAR abscisic acid receptors with the plasma membrane
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Bao Y, Aggarwa ,P, Robbins NE, Sturrock CJ, Thompson MC, Tan HQ, Tham C, Dua L, Rodriguez PL, Vernoux T, Mooney SJ, Bennett MJ, Dinneny JR (2014)
Plant roots use a patterning mechanism to position lateral root branches toward available water
Proceedings of the National Academy of Sciences USA 111: 9319-9324
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Irigoyen ML, Iniesto E, Rodriguez L, Puga MI, Yanagawa Y, Pick E, Strickland E, Paz-Ares J, Wei N, De Jaeger G, Rodriguez PL, Deng XW, Rubio V (2014)
Targeted degradation of abscisic acid receptors is mediated by the ubiquitin ligase substrate adaptor DDA1 in Arabidopis
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Bueso E, Rodriguez L, Lorenzo-Orts L, Gonzalez-Guzman M, Sayas E, Muñoz-Bertomeu J, Ibañez C, Serrano R, Rodriguez PL (2014)
The single subunit RING-type E3 ubiquitin ligase RSL1 targets PYL4 and PYR1 ABA receptors in plasma membrane to modulate abscisic acid signaling
Plant Journal 80: 1057-1071
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Gonzalez-Guzman M, Rodriguez L, Lorenzo-Orts L, Pons C, Sarrion-Perdigones A, Fernandez MA, Peirats-Llobet M, Forment J, Moreno-Alvero M, Cutler SR, Albert A, Granell A, RodriguezPL (2014)
Tomato PYR/PYL/RCAR abscisic acid receptors show high expression in root, differential sensitivity to the abscisic acid agonist quinabactin, and the capability to enhance plant drought resistance
Journal of Experimental Botany 65: 4451-4464
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Rodrigues A, Adamo M, Crozet P, Margalha L, Confraria A, Martinho C, Elias A, Gonzalez-Guzman M, Antoni R, Rodriguez PL, Baena-González E (2013)
ABI1 and PP2CA phosphatases are negative regulators of Snf1-related protein kinase1 signaling in Arabidopsis
- Flexas J, Niinemets U, Galle A, Barbour M, Diaz-Espejo A, Galmés J, Ribas-Carbo M, Rodriguez PL, Rossello F, Soolanayakanahally R, Tomas M, Wright IJ, Farquhar GD, Medrano H (2013)
Diffusional conductances to CO2 as a target for increasing photosynthesis and photosynthetic water-use efficiency
Photosynthesis Research 117: 45-59
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Locascio A, Blazquez MA, Alabadi D (2013)
Genomic Analysis of DELLA Protein Activity
Plant And Cell Physiology 54: 1229-1237
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Merilo E, Laanemets K, Hu H, Xue S, Gonzalez-Guzman M, Rodriguez PL, Schroeder JI, Brosche M, Kollist H (2013)
PYR/RCAR receptors contribute to Ozone-, Reduced Air Humidity-, Darkness- and CO2-Induced Stomatal Regulation
Plant Physiology 162: 1652-1668
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Antoni R, Gonzalez-Guzman M, Rodriguez L, Peirats-Llobet M, Pizzio Gaston A, Fernandez MA, De Winne N, De Jaeger G, Dietrich D, Bennett MJ, Rodriguez PL (2013)
PYRABACTIN RESISTANCE1-LIKE8 Plays an Important Role for the Regulation of Abscisic Acid Signaling in Root
Plant Physiology 161: 931-941
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Pizzio GA, Rodriguez L, Antoni R, Gonzalez-Guzman M, Yunta C, Merilo E, Kollist H, Albert A, Rodriguez PL (2013)
The PYL4 A194T mutant uncovers a key role of PYL4-PP2CA interaction for ABA signaling and plant drought resistance
Plant Physiology 163: 441-455
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Gonzalez-Guzman M, Pizzio GA, Antoni R, Vera-Sirera F, Merilo E, Bassel GW, Fernandez MA, Holdsworth M, Perez-Amador MA, Kollist H, Rodriguez PL (2012)
Arabidopsis PYR/PYL/RCAR Receptors Play a Major Role in Quantitative Regulation of Stomatal Aperture and Transcriptional Response to Abscisic Acid
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Wu C, Feng J, Wang R, Liu H, Yang H, Rodriguez PL, Qin H, Liu X, Wang D (2012)
HRS1 acts as a negative regulator of abscisic acid signaling to promote timely germination of Arabidopsis seeds
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Antoni R, Gonzalez-Guzman M, Rodriguez L, Rodrigues A, Pizzio GA, Rodriguez P (2012)
Selective inhibition of clade A phosphatases type 2C by PYR/PYL/RCAR abscisic acid receptors
Plant Physiology 158: 970-980
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Santiago J, Dupeux F, Betz K, Antoni R, Gonzalez-Guzman M, Rodriguez L, Marquez JA, Rodriguez PL (2012)
Structural insights into PYR/PYL/RCAR ABA receptors and PP2Cs
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Han SK, Sang Y, Rodrigues A, Wu MF, Rodriguez PL, Wagner D (2012)
The SWI2/SNF2 chromatin remodeling ATPase BRAHMA represses Abscisic Acid Responses in the Absence of the Stress Stimulus in Arabidopsis
- Dupeux F, Santiago J, Betz K, Twycross J, Park S-Y, Rodriguez L, Gonzalez-Guzman M, Jensen MR, Krasnogor N, Blackledge M, Holdsworth M, Cutler SR, Rodriguez PL, Marquez JA (2011)
A thermodynamic switch modulates abscisic acid receptor sensitivity
EMBO Journal 30: 4171-4184
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Lackman P, Gonzalez-Guzman M, Tilleman S, Carqueijeiro I, Cuellar A, Moses T, Seo M, Kanno Y, Hakkinen ST, Van Montagu MCE, Thevelein JT, Maaheimo H, Oksman-Caldentey KM, Rodriguez PL, Rischer H, Goossens A (2011)
Jasmonate signaling involves the abscisic acid receptor PYL4 to regulate metabolic reprogramming in Arabidopsis and tobacco
Proceedings of the National Academy of Sciences USA 108: 5891-5896
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Dupeux F, Antoni R, Betz,K, Santiago J, Gonzalez-Guzman M, Rodriguez L, Rubio S, Park S-Y, Cutler SR, Rodriguez PL, Marquez JA (2011)
Modulation of ABA signaling in vivo by an engineered receptor-insensitive PP2C allele
Plant Physiology 156: 106-116
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Antoni R, Rodriguez L, Gonzalez-Guzman M, Pizzio GA, Rodriguez PL (2011)
News on ABA transport, protein degradation and ABFs/WRKYs in ABA signalling
Current Opinion in Plant Biology 14: 547-553
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Cutler SR, Rodriguez PL, Finkelstein RR, Abrams SR (2010)
Abscisic acid: emergence of a core signaling network
Annual Review of Plant Biology 61: 651-679
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Vlad F, Droillard MJ, Valot B, Khafif M, Rodrigues A, Rodriguez PL, Merlot S, Laurière C (2010)
Phosphosite mapping, genetic and in planta activation studies reveal key aspects of the different phosphorylation mechanisms involved in activation of SnRK2s
Plant Journal 63: 778-790
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Park S-Y, Fung P, Nishimura N, Jensen DR, Fujii H, Zhao Y, Lumba S, Santiago J, Rodrigues A, Chow T-S, Alfred SE, Bonetta D, Finkelstein R, Provart NJ, Desveaux D, Rodriguez PL, McCourt P, Zhu J-K, Schroeder JI, Volkman BF, Cutler SR (2009)
Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of ABA-binding START proteins
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Fujii H, Chinnusamy V, Rodrigues A, Rubio S, Antoni R, Park SY, Cutler SR, Sheen J, Rodriguez PL, Zhu JK (2009)
In vitro reconstitution of an ABA signalling pathway
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Antoni,R., Dietrich,D., Bennett,M.J. and Rodriguez,P.L. (2016)Santiago, J, Rodrigues, A, Saez, A, Rubio, S, Antoni, R, Park, S, Dupeux, F, Marquez, JA, Cutler, SR, Rodriguez, PL (2009)
Modulation of drought resistance by the abscisic acid-receptor PYL5 through inhibition of clade A PP2Cs
Plant Journal 60: 575-588
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Vlad F, Rubio S, Rodrigues A, Sirichandra C, Belin C, Robert N, Leung J, Rodriguez PL Laurire C, Merlot S (2009)
Protein Phosphatases 2C Regulate the Activation of the Snf1 Related Kinase OST1 by Abscisic Acid in Arabidopsis
- Santiago J, Dupeux F, Round A, Antoni R, Park S-Y, Jamin M, Cutler SR, Rodriguez P, Marquez JA (2009)
The abscisic acid receptor PYR1 in complex with abscisic acid
Nature 462: 660-664
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Rodrigues A, Santiago J, Rubio S, Saez A, Osmont KS, Gadea J, Hardtke CS, Rodriguez PL (2009)
The short-rooted phenotype of the brevis radix mutant partly reflects root ABA hypersensitivity
Plant Physiology 149: 1917-1928
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Rubio S, Rodrigues A, Saez A, Dizon MB, Galle A, Kim T, Santiago J, Flexas J, Schroeder JIS, Rodriguez PL (2009)
Triple loss-of-function of protein phosphatases type 2C leads to partial constitutive response to endogenous ABA
Plant Physiology 150: 1345-1355
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Saez A, Rodrigues A, Santiago J, Rubio S, Rodríguez PL (2008)
HAB1-SWI3B interaction reveals a link between abscisic acid signaling and putative SWI/SNF chromatin remodeling complexes
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Barrero JM, Rodriguez PL, Quesada V, Alabadi D, Blazquez MA, Boutin JP, Marion-Poll A, Ponce MR, Micol JL (2008)
The ABA1 gene and carotenoid biosynthesis are required for late skotomorphogenic growth in Arabidopsis thaliana
Plant Cell & Environment 31: 227-234
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Rubio S, Whitehead L, Larson TR, Graham IA, Rodriguez PL (2008)
The Coenzyme A biosynthetic enzyme phosphopantetheine adenylyltransferase plays a crucial role for plant growth, salt/osmotic-stress resistance and seed lipid storage
Plant Physiology 148: 546-556
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de Torres-Zabala M, Truman W, Bennett MH, Lafforgue G, Mansfield JW, Rodriguez PL, Bogre L, Grant M (2007)
Pseudomonas syringae pv. tomato hijacks the Arabidopsis abscisic acid signalling pathway to cause disease
EMBO Journal 26: 1434-1443
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Rubio S, Larson TR, Gonzalez-Guzman M, Alejandro S, Graham IA, Serrano R, Rodriguez PL (2006)
An Arabidopsis mutant impaired in CoA biosynthesis is sugar-dependent for seedling establishment
Plant Physiology 140: 830-843
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Barrero JM, Rodriguez PL, Piqueras P, Quesada V, Ponce MR, Micol JL (2006)
Both ABA-dependent and ABA-independent pathways govern the induction of NCED3, AAO3 and ABA1 in response to salt stress
Plant Cell & Environment 29: 2000-2008
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Saez A, Robert N, Maktabi M, Schroeder JI, Serrano R, Rodriguez PL (2006)
Enhancement of abscisic acid sensitivity and reduction of water consumption in Arabidopsis by combined inactivation of the protein phosphatases type 2C ABI1 and HAB1
Plant Physiology 141: 1389-1399
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Barrero JM, Piqueras P, Gonzalez-Guzman M, Serrano R, Rodriguez PL, Ponce MR, Micol JL (2005)
A mutational analysis of the ABA1 gene of Arabidopsis thaliana highlights the involvement of ABA in vegetative development
Journal of Experimental Botany 56: 2071-2083
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Forment J, Gadea J, Huerta L, Abizanda L, Agusti J, Alamar S, Alos E, Andres F, Arribas R, Beltran JP, Berbel A, Blazquez MA, Brumos J, Canas LA, Cercos M, Colmenero-Flores JM, Conesa M, Estables B, Gandia M, Garcia-Martinez JL, Gimeno J, Gisbert A, Gomez G, Gonzalez-Candelas L, Granell A, Guerri J, Lafuente MT, Madueno F, Marcos JF, Marques MC, Martinez F, Martinez-Godoy MA, Miralles S, Moreno P, Navarro L, Pallas V, Perez-Amador MA, Perez-Valle J, Pons C, Rodrigo I, Rodriguez PL, Royo C, Serrano R, Soler G, Tadeo F, Talon M, Terol J, Trenor M, Vaello L, Vicente O, Vidal Ch, Zacarias L, Conejero V (2005)
Development of a Citrus genome-wide EST collection and cDNA microarray as resources for genomic studies
Plant Molecular Biology 57: 375-391
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Saez A, Apostolova N, Gonzalez-Guzman M, Gonzalez-Garcia MP, Nicolas C,Lorenzo O, Rodriguez PL (2004)
Gain-of-function and loss-of-function phenotypes of the protein phosphatase 2C HAB1 reveal its role as a negative regulator of abscisic acid signalling
Plant Journal 37: 354-369
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Gonzalez-Guzman M, Abia D, Salinas J, Serrano R, Rodriguez PL (2004)
Two New Alleles of the abscisic aldehyde oxidase 3 Gene Reveal Its Role in Abscisic Acid Biosynthesis in Seeds
Plant Physiology 135: 325-33
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Gonzalez-Garcia MP, Rodriguez D, Nicolas C, Rodriguez PL, Nicolas G, Lorenzo O (2003)
Negative regulation of abscisic acid signaling by the Fagus sylvatica FsPP2C1 plays a role in seed dormancy regulation and promotion of seed germination
Plant Physiology 133: 135-44
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Meskiene I, Baudouin E, Schweighofer A, Liwosz A, Jonak C, Rodriguez PL, Jelinek H, Hirt H (2003)
Stress-induced protein phosphatase 2C is a negative regulator of a mitogen-activated protein kinase
Journal of Biological Chemistry 278: 18945-18952
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Serrano R, Rodriguez PL (2002)
Plants, genes and ions. Workshop on the molecular basis of ionic homeostasis and salt tolerance in plants
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Gonzalez-Guzman M, Apostolova N, Belles JM, Barrero JM, Piqueras P, Ponce MR, Micol JL, Serrano R Rodriguez PL (2002)
The short-chain alcohol dehydrogenase ABA2 catalyzes the conversion of xanthoxin to abscisic aldehyde
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Andrade MA, Gonzalez-Guzman M, Serrano R, Rodriguez PL (2001)
A combination of the F-box motif and kelch repeats defines a large Arabidopsis family of F-box proteins
Plant Molecular Biology 46: 603-614
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Lorenzo O, Rodriguez D, Nicolas G, Rodriguez PL, Nicolas C (2001)
A new protein phosphatase 2C (FsPP2C1) induced by abscisic acid is specifically expressed in dormant beechnut seeds
Plant Physiology 125: 1949-1956
- Lopez-Coronado JM, Belles JM, Lesage F, Serrano R, Rodríguez PL (1999)
A novel mammalian lithium-sensitive enzyme with a dual enzymatic activity, 3 ‘-phosphoadenosine 5 ‘-phosphate phosphatase and inositol-polyphosphate 1-phosphatase
Journal of Biological Chemistry 274: 16034-16039
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Gil-Mascarell R, Lopez-Coronado JM, Belles JM, Serrano R, Rodríguez PL (1999)
The arabidopsis hal2-like gene family includes a novel sodium-sensitive phosphatase
Plant Journal 17: 373-383
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Rodriguez Pl, Leube Mp, Grill E (1998)
Molecular cloning in arabidopsis thaliana of a new protein phosphatase 2C (PP2C) with homology to ABI1 and ABI2
Plant Molecular Biology 38: 879-
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Protein phosphatase 2C (PP2C) function in higher plants
Plant Molecular Biology 38: 919-927
