Fabio Pasin’s studies focus on the family Potyviridae, a major group of plant viruses, and have highlighted cleavage of viral polyproteins as a key immune evasion mechanism that coordinates virus replication and host-virus interaction. His recent research seeks to combine synthetic biology principles and data-driven approaches for viral system design and tool development. His work outputs were published by peer-reviewed journals in the fields of plant science, virology, applied microbiology and biotechnology. He is the inventor of international patent applications.
MAIN PUBLICATIONS
Pasin F* (2022) Assembly of plant virus agroinfectious clones using biological material or DNA synthesis. STAR Protoc. 3(4):101716. doi:10.1016/j.xpro.2022.101716
Pasin F*, Daròs J-A, Tzanetakis IE (2022) Proteome expansion in the Potyviridae evolutionary radiation. FEMS Microbiol Rev. 46(4):fuac011. doi:10.1093/femsre/fuac011
Yue J, Wei Y, Sun Z, Chen Y, Wei X, Wang H, Pasin F*, Zhao M* (2022) AlkB RNA demethylase homologues and N6-methyladenosine are involved in Potyvirus infection. Mol Plant Pathol. 23(10):1555-1564. doi:10.1111/mpp.13239
Aragonés V, Aliaga F, Pasin F, Daròs J-A (2022) Simplifying plant gene silencing and genome editing logistics by a one-Agrobacterium system for simultaneous delivery of multipartite virus vectors. Biotechnol J. 17(7):e2100504. doi:10.1002/biot.202100504
Pasin F* (2021) Oligonucleotide abundance biases aid design of a type IIS synthetic genomics framework with plant virome capacity. Biotechnol J. 16:e2000354. doi:10.1002/biot.202000354
Pasin F*, Shan H, García B, Müller M, San León D, Ludman M, Fresno DH, Fátyol K, Munné-Bosch S, Rodrigo G, García JA (2020) Abscisic acid connects phytohormone signaling with RNA metabolic pathways and promotes an antiviral response that is evaded by a self-controlled RNA virus. Plant Commun. 1:100099. doi:10.1016/j.xplc.2020.100099
Pasin F*, Menzel W, Daròs J-A (2019) Harnessed viruses in the age of metagenomics and synthetic biology: an update on infectious clone assembly and biotechnologies of plant viruses. Plant Biotechnol J. 17:1010–1026. doi:10.1111/pbi.13084
Pasin F*, Bedoya LC, Bernabé-Orts JM, Gallo A, Simón-Mateo C, Orzaez D, García JA (2017) Multiple T-DNA delivery to plants using novel mini binary vectors with compatible replication origins. ACS Synth Biol. 6:1962–1968. doi:10.1021/acssynbio.6b00354
PATENTS
Pasin F, García Alvarez JA, Bedoya Rojas LC, Simón Mateo MdC, Orzáez Calatayud DV, Bernabé Orts JM. Binary vectors and uses of same. Pat. no. US 11,597,939 B2. Status: Granted on March 7, 2023
