Fabio Pasin’s studies focus on the family Potyviridae, a major group of plant viruses, and have highlighted cleavage of viral polyproteins as a key immune evasion mechanism that coordinates virus replication and host-virus interaction. His recent research seeks to combine synthetic biology principles and data-driven approaches for viral system design and tool development. His work outputs were published by peer-reviewed journals in the fields of plant science, virology, applied microbiology and biotechnology. He is the inventor of international patent applications.
Pasin F* (2022) Assembly of plant virus agroinfectious clones using biological material or DNA synthesis. STAR Protoc. 3(4):101716. doi:10.1016/j.xpro.2022.101716
Pasin F*, Daròs J-A, Tzanetakis IE (2022) Proteome expansion in the Potyviridae evolutionary radiation. FEMS Microbiol Rev. 46(4):fuac011. doi:10.1093/femsre/fuac011
Yue J, Wei Y, Sun Z, Chen Y, Wei X, Wang H, Pasin F*, Zhao M* (2022) AlkB RNA demethylase homologues and N6-methyladenosine are involved in Potyvirus infection. Mol Plant Pathol. 23(10):1555-1564. doi:10.1111/mpp.13239
Aragonés V, Aliaga F, Pasin F, Daròs J-A (2022) Simplifying plant gene silencing and genome editing logistics by a one-Agrobacterium system for simultaneous delivery of multipartite virus vectors. Biotechnol J. 17(7):e2100504. doi:10.1002/biot.202100504
Pasin F* (2021) Oligonucleotide abundance biases aid design of a type IIS synthetic genomics framework with plant virome capacity. Biotechnol J. 16:e2000354. doi:10.1002/biot.202000354
Pasin F*, Shan H, García B, Müller M, San León D, Ludman M, Fresno DH, Fátyol K, Munné-Bosch S, Rodrigo G, García JA (2020) Abscisic acid connects phytohormone signaling with RNA metabolic pathways and promotes an antiviral response that is evaded by a self-controlled RNA virus. Plant Commun. 1:100099. doi:10.1016/j.xplc.2020.100099
Pasin F*, Menzel W, Daròs J-A (2019) Harnessed viruses in the age of metagenomics and synthetic biology: an update on infectious clone assembly and biotechnologies of plant viruses. Plant Biotechnol J. 17:1010–1026. doi:10.1111/pbi.13084
Pasin F*, Bedoya LC, Bernabé-Orts JM, Gallo A, Simón-Mateo C, Orzaez D, García JA (2017) Multiple T-DNA delivery to plants using novel mini binary vectors with compatible replication origins. ACS Synth Biol. 6:1962–1968. doi:10.1021/acssynbio.6b00354
